Entrapment of Cultured Pancreas Islets in Three-Dimensional Collagen Matrices
In vitro culture of islets of Langerhans decreases their immunogenecity, presumably by eliminating passenger leukocytes and other Ia+ presenting cells within the islets. Islets cultivated in petri dishes either at 37°C or at 25°C gradually disintegrate during culture in a time-dependent manner which...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
1992-01-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.1177/096368979200100109 |
| _version_ | 1818303841238515712 |
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| author | Seh-Huang Chao Madhusudan V. Peshwa David E.R. Sutherland M.D., Ph.D. Wei-Shou Hu |
| author_facet | Seh-Huang Chao Madhusudan V. Peshwa David E.R. Sutherland M.D., Ph.D. Wei-Shou Hu |
| author_sort | Seh-Huang Chao |
| collection | DOAJ |
| description | In vitro culture of islets of Langerhans decreases their immunogenecity, presumably by eliminating passenger leukocytes and other Ia+ presenting cells within the islets. Islets cultivated in petri dishes either at 37°C or at 25°C gradually disintegrate during culture in a time-dependent manner which is related to the free-floating condition of the islets. Also, a fraction of the islets disperse as single cells and beta-cell aggregates or adhere to the bottom of the culture dishes. Thus, the retrieval rate of transplantable islets is dampened due to their disintegration and spontaneous dispersion in conventional petri dish cultures. Entrapment of freshly harvested islets of Langerhans in a three-dimensional collagen matrix was studied as an alternative method for islet cultivation. The contraction of collagen fibrils during in vitro culture counteracts the dispersion of islets and helps in maintaining their integrity while in culture. It was observed that the entrapped islets maintain satisfactory morphology, viability, and capability of glucose-dependent insulin secretion for over 2 wk. The oxygen consumption rate and glucose metabolism of these islets was not deranged when entrapped in collagen. Also, the retrieval of islets is easier and more efficient than that observed in conventional culture systems. Our results indicate that culture of islets in three-dimensional collagen gels can potentially develop into an ideal system applicable to clinical transplantation of cultured islets or beta-cell aggregates. |
| first_indexed | 2024-12-13T06:01:12Z |
| format | Article |
| id | doaj.art-bbcfefc4625a4830925cb0501ef946d6 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-13T06:01:12Z |
| publishDate | 1992-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-bbcfefc4625a4830925cb0501ef946d62022-12-21T23:57:19ZengSAGE PublishingCell Transplantation0963-68971555-38921992-01-01110.1177/096368979200100109Entrapment of Cultured Pancreas Islets in Three-Dimensional Collagen MatricesSeh-Huang Chao0Madhusudan V. Peshwa1David E.R. Sutherland M.D., Ph.D.2Wei-Shou Hu3Department of Surgery, School of Medicine, National Taiwan University, Taiwan, ROCDepartment of Chemical Engineering and Materials ScienceDepartment of Surgery, University of Minnesota, Minneapolis, MN, USADepartment of Chemical Engineering and Materials ScienceIn vitro culture of islets of Langerhans decreases their immunogenecity, presumably by eliminating passenger leukocytes and other Ia+ presenting cells within the islets. Islets cultivated in petri dishes either at 37°C or at 25°C gradually disintegrate during culture in a time-dependent manner which is related to the free-floating condition of the islets. Also, a fraction of the islets disperse as single cells and beta-cell aggregates or adhere to the bottom of the culture dishes. Thus, the retrieval rate of transplantable islets is dampened due to their disintegration and spontaneous dispersion in conventional petri dish cultures. Entrapment of freshly harvested islets of Langerhans in a three-dimensional collagen matrix was studied as an alternative method for islet cultivation. The contraction of collagen fibrils during in vitro culture counteracts the dispersion of islets and helps in maintaining their integrity while in culture. It was observed that the entrapped islets maintain satisfactory morphology, viability, and capability of glucose-dependent insulin secretion for over 2 wk. The oxygen consumption rate and glucose metabolism of these islets was not deranged when entrapped in collagen. Also, the retrieval of islets is easier and more efficient than that observed in conventional culture systems. Our results indicate that culture of islets in three-dimensional collagen gels can potentially develop into an ideal system applicable to clinical transplantation of cultured islets or beta-cell aggregates.https://doi.org/10.1177/096368979200100109 |
| spellingShingle | Seh-Huang Chao Madhusudan V. Peshwa David E.R. Sutherland M.D., Ph.D. Wei-Shou Hu Entrapment of Cultured Pancreas Islets in Three-Dimensional Collagen Matrices Cell Transplantation |
| title | Entrapment of Cultured Pancreas Islets in Three-Dimensional Collagen Matrices |
| title_full | Entrapment of Cultured Pancreas Islets in Three-Dimensional Collagen Matrices |
| title_fullStr | Entrapment of Cultured Pancreas Islets in Three-Dimensional Collagen Matrices |
| title_full_unstemmed | Entrapment of Cultured Pancreas Islets in Three-Dimensional Collagen Matrices |
| title_short | Entrapment of Cultured Pancreas Islets in Three-Dimensional Collagen Matrices |
| title_sort | entrapment of cultured pancreas islets in three dimensional collagen matrices |
| url | https://doi.org/10.1177/096368979200100109 |
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