Mixed infection of an emaravirus, a crinivirus, and a begomovirus in Pueraria lobata (Willd) Ohwi
Pueraria lobata (Willd) (Pueraria montana var. lobata (Willd.) Maesen & S. M. Almeida ex Sanjappa & Predeep) is an important herbal medicine used in many countries. In P. lobata plants showing symptoms of mosaic, yellow spots, and mottling, mixed infection of new viruses provisionall...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-09-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.926724/full |
| _version_ | 1811208152373264384 |
|---|---|
| author | Xiaofei Liang Shiqiang Mei Haodong Yu Song Zhang Jiaxing Wu Mengji Cao |
| author_facet | Xiaofei Liang Shiqiang Mei Haodong Yu Song Zhang Jiaxing Wu Mengji Cao |
| author_sort | Xiaofei Liang |
| collection | DOAJ |
| description | Pueraria lobata (Willd) (Pueraria montana var. lobata (Willd.) Maesen & S. M. Almeida ex Sanjappa & Predeep) is an important herbal medicine used in many countries. In P. lobata plants showing symptoms of mosaic, yellow spots, and mottling, mixed infection of new viruses provisionally named Pueraria lobata-associated emaravirus (PloAEV, genus Emaravirus), Pueraria lobata-associated crinivirus (PloACV, genus Crinivirus), and isolate CQ of the previously reported kudzu mosaic virus (KuMV-CQ, genus Begomovirus) was confirmed through high-throughput sequencing. PloAEV has five RNA segments, encoding a putative RNA-dependent RNA polymerase, glycoprotein precursor, nucleocapsid protein, movement protein, and P5, respectively. PloACV has two RNA segments, encoding 11 putative proteins. Only PloAEV could be mechanically transmitted from mixed infected symptomatic kudzu to Nicotiana benthamiana plants. All three viruses were detected in 35 symptomatic samples collected from five different growing areas, whereas no viruses were detected in 21 non-symptomatic plants, suggesting a high association between these three viruses. Thus, this study provides new knowledge on the diversity and molecular characteristics of viruses in P. lobata plants affected by the viral disease. |
| first_indexed | 2024-04-12T04:16:59Z |
| format | Article |
| id | doaj.art-bbd5088e97644133a955cfbe92578f91 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T04:16:59Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-bbd5088e97644133a955cfbe92578f912022-12-22T03:48:23ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-09-011310.3389/fmicb.2022.926724926724Mixed infection of an emaravirus, a crinivirus, and a begomovirus in Pueraria lobata (Willd) OhwiXiaofei LiangShiqiang MeiHaodong YuSong ZhangJiaxing WuMengji CaoPueraria lobata (Willd) (Pueraria montana var. lobata (Willd.) Maesen & S. M. Almeida ex Sanjappa & Predeep) is an important herbal medicine used in many countries. In P. lobata plants showing symptoms of mosaic, yellow spots, and mottling, mixed infection of new viruses provisionally named Pueraria lobata-associated emaravirus (PloAEV, genus Emaravirus), Pueraria lobata-associated crinivirus (PloACV, genus Crinivirus), and isolate CQ of the previously reported kudzu mosaic virus (KuMV-CQ, genus Begomovirus) was confirmed through high-throughput sequencing. PloAEV has five RNA segments, encoding a putative RNA-dependent RNA polymerase, glycoprotein precursor, nucleocapsid protein, movement protein, and P5, respectively. PloACV has two RNA segments, encoding 11 putative proteins. Only PloAEV could be mechanically transmitted from mixed infected symptomatic kudzu to Nicotiana benthamiana plants. All three viruses were detected in 35 symptomatic samples collected from five different growing areas, whereas no viruses were detected in 21 non-symptomatic plants, suggesting a high association between these three viruses. Thus, this study provides new knowledge on the diversity and molecular characteristics of viruses in P. lobata plants affected by the viral disease.https://www.frontiersin.org/articles/10.3389/fmicb.2022.926724/fullHTSmechanical inoculationPueraria lobataEmaravirusmixed infection |
| spellingShingle | Xiaofei Liang Shiqiang Mei Haodong Yu Song Zhang Jiaxing Wu Mengji Cao Mixed infection of an emaravirus, a crinivirus, and a begomovirus in Pueraria lobata (Willd) Ohwi Frontiers in Microbiology HTS mechanical inoculation Pueraria lobata Emaravirus mixed infection |
| title | Mixed infection of an emaravirus, a crinivirus, and a begomovirus in Pueraria lobata (Willd) Ohwi |
| title_full | Mixed infection of an emaravirus, a crinivirus, and a begomovirus in Pueraria lobata (Willd) Ohwi |
| title_fullStr | Mixed infection of an emaravirus, a crinivirus, and a begomovirus in Pueraria lobata (Willd) Ohwi |
| title_full_unstemmed | Mixed infection of an emaravirus, a crinivirus, and a begomovirus in Pueraria lobata (Willd) Ohwi |
| title_short | Mixed infection of an emaravirus, a crinivirus, and a begomovirus in Pueraria lobata (Willd) Ohwi |
| title_sort | mixed infection of an emaravirus a crinivirus and a begomovirus in pueraria lobata willd ohwi |
| topic | HTS mechanical inoculation Pueraria lobata Emaravirus mixed infection |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.926724/full |
| work_keys_str_mv | AT xiaofeiliang mixedinfectionofanemaravirusacrinivirusandabegomovirusinpuerarialobatawilldohwi AT shiqiangmei mixedinfectionofanemaravirusacrinivirusandabegomovirusinpuerarialobatawilldohwi AT haodongyu mixedinfectionofanemaravirusacrinivirusandabegomovirusinpuerarialobatawilldohwi AT songzhang mixedinfectionofanemaravirusacrinivirusandabegomovirusinpuerarialobatawilldohwi AT jiaxingwu mixedinfectionofanemaravirusacrinivirusandabegomovirusinpuerarialobatawilldohwi AT mengjicao mixedinfectionofanemaravirusacrinivirusandabegomovirusinpuerarialobatawilldohwi |