Macrophages are necessary for epimorphic regeneration in African spiny mice
How the immune system affects tissue regeneration is not well understood. In this study, we used an emerging mammalian model of epimorphic regeneration, the African spiny mouse, to examine cell-based inflammation and tested the hypothesis that macrophages are necessary for regeneration. By directly...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2017-05-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/24623 |
| _version_ | 1811236982986113024 |
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| author | Jennifer Simkin Thomas R Gawriluk John C Gensel Ashley W Seifert |
| author_facet | Jennifer Simkin Thomas R Gawriluk John C Gensel Ashley W Seifert |
| author_sort | Jennifer Simkin |
| collection | DOAJ |
| description | How the immune system affects tissue regeneration is not well understood. In this study, we used an emerging mammalian model of epimorphic regeneration, the African spiny mouse, to examine cell-based inflammation and tested the hypothesis that macrophages are necessary for regeneration. By directly comparing inflammatory cell activation in a 4 mm ear injury during regeneration (Acomys cahirinus) and scarring (Mus musculus), we found that both species exhibited an acute inflammatory response, with scarring characterized by stronger myeloperoxidase activity. In contrast, ROS production was stronger and more persistent during regeneration. By depleting macrophages during injury, we demonstrate a functional requirement for these cells to stimulate regeneration. Importantly, the spatial distribution of activated macrophage subtypes was unique during regeneration with pro-inflammatory macrophages failing to infiltrate the regeneration blastema. Together, our results demonstrate an essential role for inflammatory cells to regulate a regenerative response. |
| first_indexed | 2024-04-12T12:18:07Z |
| format | Article |
| id | doaj.art-bbd57cb6248d4347bc890fabd4d41f09 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T12:18:07Z |
| publishDate | 2017-05-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-bbd57cb6248d4347bc890fabd4d41f092022-12-22T03:33:22ZengeLife Sciences Publications LtdeLife2050-084X2017-05-01610.7554/eLife.24623Macrophages are necessary for epimorphic regeneration in African spiny miceJennifer Simkin0https://orcid.org/0000-0002-6122-4222Thomas R Gawriluk1https://orcid.org/0000-0001-9868-9704John C Gensel2https://orcid.org/0000-0001-8980-108XAshley W Seifert3https://orcid.org/0000-0001-6576-3664Department of Biology, University of Kentucky, Lexington, United States; Department of Physiology, University of Kentucky, Lexington, United States; Spinal Cord and Brain Injury Research Center, University of Kentucky, Lexington, United StatesDepartment of Biology, University of Kentucky, Lexington, United StatesDepartment of Physiology, University of Kentucky, Lexington, United States; Spinal Cord and Brain Injury Research Center, University of Kentucky, Lexington, United StatesDepartment of Biology, University of Kentucky, Lexington, United StatesHow the immune system affects tissue regeneration is not well understood. In this study, we used an emerging mammalian model of epimorphic regeneration, the African spiny mouse, to examine cell-based inflammation and tested the hypothesis that macrophages are necessary for regeneration. By directly comparing inflammatory cell activation in a 4 mm ear injury during regeneration (Acomys cahirinus) and scarring (Mus musculus), we found that both species exhibited an acute inflammatory response, with scarring characterized by stronger myeloperoxidase activity. In contrast, ROS production was stronger and more persistent during regeneration. By depleting macrophages during injury, we demonstrate a functional requirement for these cells to stimulate regeneration. Importantly, the spatial distribution of activated macrophage subtypes was unique during regeneration with pro-inflammatory macrophages failing to infiltrate the regeneration blastema. Together, our results demonstrate an essential role for inflammatory cells to regulate a regenerative response.https://elifesciences.org/articles/24623Acomysepimorphic regenerationmacrophagesinflammation |
| spellingShingle | Jennifer Simkin Thomas R Gawriluk John C Gensel Ashley W Seifert Macrophages are necessary for epimorphic regeneration in African spiny mice eLife Acomys epimorphic regeneration macrophages inflammation |
| title | Macrophages are necessary for epimorphic regeneration in African spiny mice |
| title_full | Macrophages are necessary for epimorphic regeneration in African spiny mice |
| title_fullStr | Macrophages are necessary for epimorphic regeneration in African spiny mice |
| title_full_unstemmed | Macrophages are necessary for epimorphic regeneration in African spiny mice |
| title_short | Macrophages are necessary for epimorphic regeneration in African spiny mice |
| title_sort | macrophages are necessary for epimorphic regeneration in african spiny mice |
| topic | Acomys epimorphic regeneration macrophages inflammation |
| url | https://elifesciences.org/articles/24623 |
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