Gut Digestive Function and Microbiome after Correction of Experimental Dysbiosis in Rats by Indigenous Bifidobacteria
In recent years, great interest has arisen in the use of autoprobiotics (indigenous bacteria isolated from the organism and introduced into the same organism after growing). This study aimed to evaluate the effects of indigenous bifidobacteria on intestinal microbiota and digestive enzymes in a rat...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-03-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/9/3/522 |
| _version_ | 1827603355689549824 |
|---|---|
| author | Lyudmila V. Gromova Elena I. Ermolenko Anastasiya L. Sepp Yulia V. Dmitrieva Anna S. Alekseeva Nadezhda S. Lavrenova Mariya P. Kotyleva Tatyana A. Kramskaya Alena B. Karaseva Alexandr N. Suvorov Andrey A. Gruzdkov |
| author_facet | Lyudmila V. Gromova Elena I. Ermolenko Anastasiya L. Sepp Yulia V. Dmitrieva Anna S. Alekseeva Nadezhda S. Lavrenova Mariya P. Kotyleva Tatyana A. Kramskaya Alena B. Karaseva Alexandr N. Suvorov Andrey A. Gruzdkov |
| author_sort | Lyudmila V. Gromova |
| collection | DOAJ |
| description | In recent years, great interest has arisen in the use of autoprobiotics (indigenous bacteria isolated from the organism and introduced into the same organism after growing). This study aimed to evaluate the effects of indigenous bifidobacteria on intestinal microbiota and digestive enzymes in a rat model of antibiotic-associated dysbiosis. Our results showed that indigenous bifidobacteria (the Bf group) accelerate the disappearance of dyspeptic symptoms in rats and prevent an increase in chyme mass in the upper intestine compared to the group without autoprobiotics (the C1 group), but significantly increase the mass of chyme in the colon compared to the C1 group and the control group (healthy animals). In the Bf group in the gut microbiota, the content of opportunistic bacteria (<i>Proteus</i> spp., enteropathogenic <i>Escherichia coli</i>) decreased, and the content of some beneficial bacteria (<i>Bifidobacterium</i> spp., <i>Dorea</i> spp., <i>Blautia</i> spp., the genus <i>Ruminococcus</i>, <i>Prevotella</i>, <i>Oscillospira</i>) changed compared to the control group. Unlike the C1 group, in the Bf group there was no decrease in the specific activities of maltase and alkaline phosphatase in the mucosa of the upper intestine, but the specific activity of maltase was decreased in the colon chyme compared to the control and C1 groups. In the Bf group, the specific activity of aminopeptidase N was reduced in the duodenum mucosa and the colon chyme compared to the control group. We concluded that indigenous bifidobacteria can protect the microbiota and intestinal digestive enzymes in the intestine from disorders caused by dysbiosis; however, there may be impaired motor function of the colon. |
| first_indexed | 2024-03-09T05:37:06Z |
| format | Article |
| id | doaj.art-bbd78abd1e264d5f88afacede8d8e4c6 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T05:37:06Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-bbd78abd1e264d5f88afacede8d8e4c62023-12-03T12:27:49ZengMDPI AGMicroorganisms2076-26072021-03-019352210.3390/microorganisms9030522Gut Digestive Function and Microbiome after Correction of Experimental Dysbiosis in Rats by Indigenous BifidobacteriaLyudmila V. Gromova0Elena I. Ermolenko1Anastasiya L. Sepp2Yulia V. Dmitrieva3Anna S. Alekseeva4Nadezhda S. Lavrenova5Mariya P. Kotyleva6Tatyana A. Kramskaya7Alena B. Karaseva8Alexandr N. Suvorov9Andrey A. Gruzdkov10Pavlov Institute of Physiology, Russian Academy of Sciences, 199034 Saint-Petersburg, RussiaDepartment of Molecular Microbiology, Institute of Experimental Medicine, 197376 Saint-Petersburg, RussiaPavlov Institute of Physiology, Russian Academy of Sciences, 199034 Saint-Petersburg, RussiaPavlov Institute of Physiology, Russian Academy of Sciences, 199034 Saint-Petersburg, RussiaPavlov Institute of Physiology, Russian Academy of Sciences, 199034 Saint-Petersburg, RussiaDepartment of Molecular Microbiology, Institute of Experimental Medicine, 197376 Saint-Petersburg, RussiaDepartment of Molecular Microbiology, Institute of Experimental Medicine, 197376 Saint-Petersburg, RussiaDepartment of Molecular Microbiology, Institute of Experimental Medicine, 197376 Saint-Petersburg, RussiaDepartment of Molecular Microbiology, Institute of Experimental Medicine, 197376 Saint-Petersburg, RussiaDepartment of Molecular Microbiology, Institute of Experimental Medicine, 197376 Saint-Petersburg, RussiaPavlov Institute of Physiology, Russian Academy of Sciences, 199034 Saint-Petersburg, RussiaIn recent years, great interest has arisen in the use of autoprobiotics (indigenous bacteria isolated from the organism and introduced into the same organism after growing). This study aimed to evaluate the effects of indigenous bifidobacteria on intestinal microbiota and digestive enzymes in a rat model of antibiotic-associated dysbiosis. Our results showed that indigenous bifidobacteria (the Bf group) accelerate the disappearance of dyspeptic symptoms in rats and prevent an increase in chyme mass in the upper intestine compared to the group without autoprobiotics (the C1 group), but significantly increase the mass of chyme in the colon compared to the C1 group and the control group (healthy animals). In the Bf group in the gut microbiota, the content of opportunistic bacteria (<i>Proteus</i> spp., enteropathogenic <i>Escherichia coli</i>) decreased, and the content of some beneficial bacteria (<i>Bifidobacterium</i> spp., <i>Dorea</i> spp., <i>Blautia</i> spp., the genus <i>Ruminococcus</i>, <i>Prevotella</i>, <i>Oscillospira</i>) changed compared to the control group. Unlike the C1 group, in the Bf group there was no decrease in the specific activities of maltase and alkaline phosphatase in the mucosa of the upper intestine, but the specific activity of maltase was decreased in the colon chyme compared to the control and C1 groups. In the Bf group, the specific activity of aminopeptidase N was reduced in the duodenum mucosa and the colon chyme compared to the control group. We concluded that indigenous bifidobacteria can protect the microbiota and intestinal digestive enzymes in the intestine from disorders caused by dysbiosis; however, there may be impaired motor function of the colon.https://www.mdpi.com/2076-2607/9/3/522autoprobioticsintestinal digestive enzymesmicrobiome |
| spellingShingle | Lyudmila V. Gromova Elena I. Ermolenko Anastasiya L. Sepp Yulia V. Dmitrieva Anna S. Alekseeva Nadezhda S. Lavrenova Mariya P. Kotyleva Tatyana A. Kramskaya Alena B. Karaseva Alexandr N. Suvorov Andrey A. Gruzdkov Gut Digestive Function and Microbiome after Correction of Experimental Dysbiosis in Rats by Indigenous Bifidobacteria Microorganisms autoprobiotics intestinal digestive enzymes microbiome |
| title | Gut Digestive Function and Microbiome after Correction of Experimental Dysbiosis in Rats by Indigenous Bifidobacteria |
| title_full | Gut Digestive Function and Microbiome after Correction of Experimental Dysbiosis in Rats by Indigenous Bifidobacteria |
| title_fullStr | Gut Digestive Function and Microbiome after Correction of Experimental Dysbiosis in Rats by Indigenous Bifidobacteria |
| title_full_unstemmed | Gut Digestive Function and Microbiome after Correction of Experimental Dysbiosis in Rats by Indigenous Bifidobacteria |
| title_short | Gut Digestive Function and Microbiome after Correction of Experimental Dysbiosis in Rats by Indigenous Bifidobacteria |
| title_sort | gut digestive function and microbiome after correction of experimental dysbiosis in rats by indigenous bifidobacteria |
| topic | autoprobiotics intestinal digestive enzymes microbiome |
| url | https://www.mdpi.com/2076-2607/9/3/522 |
| work_keys_str_mv | AT lyudmilavgromova gutdigestivefunctionandmicrobiomeaftercorrectionofexperimentaldysbiosisinratsbyindigenousbifidobacteria AT elenaiermolenko gutdigestivefunctionandmicrobiomeaftercorrectionofexperimentaldysbiosisinratsbyindigenousbifidobacteria AT anastasiyalsepp gutdigestivefunctionandmicrobiomeaftercorrectionofexperimentaldysbiosisinratsbyindigenousbifidobacteria AT yuliavdmitrieva gutdigestivefunctionandmicrobiomeaftercorrectionofexperimentaldysbiosisinratsbyindigenousbifidobacteria AT annasalekseeva gutdigestivefunctionandmicrobiomeaftercorrectionofexperimentaldysbiosisinratsbyindigenousbifidobacteria AT nadezhdaslavrenova gutdigestivefunctionandmicrobiomeaftercorrectionofexperimentaldysbiosisinratsbyindigenousbifidobacteria AT mariyapkotyleva gutdigestivefunctionandmicrobiomeaftercorrectionofexperimentaldysbiosisinratsbyindigenousbifidobacteria AT tatyanaakramskaya gutdigestivefunctionandmicrobiomeaftercorrectionofexperimentaldysbiosisinratsbyindigenousbifidobacteria AT alenabkaraseva gutdigestivefunctionandmicrobiomeaftercorrectionofexperimentaldysbiosisinratsbyindigenousbifidobacteria AT alexandrnsuvorov gutdigestivefunctionandmicrobiomeaftercorrectionofexperimentaldysbiosisinratsbyindigenousbifidobacteria AT andreyagruzdkov gutdigestivefunctionandmicrobiomeaftercorrectionofexperimentaldysbiosisinratsbyindigenousbifidobacteria |