Qualitative Analysis and Componential Differences of Chemical Constituents in Taxilli Herba from Different Hosts by UFLC-Triple TOF-MS/MS
Taxilli Herba (TH) is a well-known traditional Chinese medicine (TCM) with a wide range of clinical application. However, there is a lack of comprehensive research on its chemical composition in recent years. At the same time, <i>Taxillus chinensis</i> (DC) Danser is a semi parasitic pla...
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MDPI AG
2021-10-01
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| Series: | Molecules |
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| author | Jiahuan Yuan Li Li Zhichen Cai Nan Wu Cuihua Chen Shengxin Yin Shengjin Liu Wenxin Wang Yuqi Mei Lifang Wei Xunhong Liu Lisi Zou Haijie Chen |
| author_facet | Jiahuan Yuan Li Li Zhichen Cai Nan Wu Cuihua Chen Shengxin Yin Shengjin Liu Wenxin Wang Yuqi Mei Lifang Wei Xunhong Liu Lisi Zou Haijie Chen |
| author_sort | Jiahuan Yuan |
| collection | DOAJ |
| description | Taxilli Herba (TH) is a well-known traditional Chinese medicine (TCM) with a wide range of clinical application. However, there is a lack of comprehensive research on its chemical composition in recent years. At the same time, <i>Taxillus chinensis</i> (DC) Danser is a semi parasitic plant with abundant hosts, and its chemical constituents varies due to hosts. In this study, the characterization of chemical constituents in TH was analyzed by ultra-fast liquid chromatography coupled with triple quadrupole-time of flight tandem mass spectrometry (UFLC-Triple TOF-MS/MS). Moreover, partial least squares discriminant analysis (PLS-DA) was applied to reveal the differential constituents in TH from different hosts based on the qualitative information of the chemical constituents. Results showed that 73 constituents in TH were identified or tentatively presumed, including flavonoids, phenolic acids and glycosides, and others; meanwhile, the fragmentation pathways of different types of compounds were preliminarily deduced by the fragmentation behavior of the major constituents. In addition, 23 differential characteristic constituents were screened based on variable importance in projection (VIP) and <i>p</i>-value. Among them, quercetin 3-<i>O</i>-<i>β</i>-D-glucuronide, quercitrin and hyperoside were common differential constituents. Our research will contribute to comprehensive evaluation and intrinsic quality control of TH, and provide a scientific basis for the variety identification of medicinal materials from different hosts. |
| first_indexed | 2024-03-10T05:56:08Z |
| format | Article |
| id | doaj.art-bbda8604449a48f8ae0806a2c584bba1 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T05:56:08Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-bbda8604449a48f8ae0806a2c584bba12023-11-22T21:20:20ZengMDPI AGMolecules1420-30492021-10-012621637310.3390/molecules26216373Qualitative Analysis and Componential Differences of Chemical Constituents in Taxilli Herba from Different Hosts by UFLC-Triple TOF-MS/MSJiahuan Yuan0Li Li1Zhichen Cai2Nan Wu3Cuihua Chen4Shengxin Yin5Shengjin Liu6Wenxin Wang7Yuqi Mei8Lifang Wei9Xunhong Liu10Lisi Zou11Haijie Chen12College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaCollege of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530220, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaTaxilli Herba (TH) is a well-known traditional Chinese medicine (TCM) with a wide range of clinical application. However, there is a lack of comprehensive research on its chemical composition in recent years. At the same time, <i>Taxillus chinensis</i> (DC) Danser is a semi parasitic plant with abundant hosts, and its chemical constituents varies due to hosts. In this study, the characterization of chemical constituents in TH was analyzed by ultra-fast liquid chromatography coupled with triple quadrupole-time of flight tandem mass spectrometry (UFLC-Triple TOF-MS/MS). Moreover, partial least squares discriminant analysis (PLS-DA) was applied to reveal the differential constituents in TH from different hosts based on the qualitative information of the chemical constituents. Results showed that 73 constituents in TH were identified or tentatively presumed, including flavonoids, phenolic acids and glycosides, and others; meanwhile, the fragmentation pathways of different types of compounds were preliminarily deduced by the fragmentation behavior of the major constituents. In addition, 23 differential characteristic constituents were screened based on variable importance in projection (VIP) and <i>p</i>-value. Among them, quercetin 3-<i>O</i>-<i>β</i>-D-glucuronide, quercitrin and hyperoside were common differential constituents. Our research will contribute to comprehensive evaluation and intrinsic quality control of TH, and provide a scientific basis for the variety identification of medicinal materials from different hosts.https://www.mdpi.com/1420-3049/26/21/6373Taxilli Herbahostschemical constituentsUFLC-Triple TOF-MS/MS |
| spellingShingle | Jiahuan Yuan Li Li Zhichen Cai Nan Wu Cuihua Chen Shengxin Yin Shengjin Liu Wenxin Wang Yuqi Mei Lifang Wei Xunhong Liu Lisi Zou Haijie Chen Qualitative Analysis and Componential Differences of Chemical Constituents in Taxilli Herba from Different Hosts by UFLC-Triple TOF-MS/MS Molecules Taxilli Herba hosts chemical constituents UFLC-Triple TOF-MS/MS |
| title | Qualitative Analysis and Componential Differences of Chemical Constituents in Taxilli Herba from Different Hosts by UFLC-Triple TOF-MS/MS |
| title_full | Qualitative Analysis and Componential Differences of Chemical Constituents in Taxilli Herba from Different Hosts by UFLC-Triple TOF-MS/MS |
| title_fullStr | Qualitative Analysis and Componential Differences of Chemical Constituents in Taxilli Herba from Different Hosts by UFLC-Triple TOF-MS/MS |
| title_full_unstemmed | Qualitative Analysis and Componential Differences of Chemical Constituents in Taxilli Herba from Different Hosts by UFLC-Triple TOF-MS/MS |
| title_short | Qualitative Analysis and Componential Differences of Chemical Constituents in Taxilli Herba from Different Hosts by UFLC-Triple TOF-MS/MS |
| title_sort | qualitative analysis and componential differences of chemical constituents in taxilli herba from different hosts by uflc triple tof ms ms |
| topic | Taxilli Herba hosts chemical constituents UFLC-Triple TOF-MS/MS |
| url | https://www.mdpi.com/1420-3049/26/21/6373 |
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