Sophora flavescens Aiton Decreases MPP+-Induced Mitochondrial Dysfunction in SH-SY5Y Cells
Sophora flavescens Aiton (SF) has been used to treat various diseases including fever and inflammation in China, South Korea and Japan. Several recent reports have shown that SF has anti-inflammatory and anti-apoptotic effects, indicating that it is a promising candidate for treatment of Parkinson’s...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-04-01
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| Series: | Frontiers in Aging Neuroscience |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fnagi.2018.00119/full |
| _version_ | 1811191908694753280 |
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| author | Hee-Young Kim Hyongjun Jeon Hyungwoo Kim Sungtae Koo Sungtae Koo Seungtae Kim Seungtae Kim |
| author_facet | Hee-Young Kim Hyongjun Jeon Hyungwoo Kim Sungtae Koo Sungtae Koo Seungtae Kim Seungtae Kim |
| author_sort | Hee-Young Kim |
| collection | DOAJ |
| description | Sophora flavescens Aiton (SF) has been used to treat various diseases including fever and inflammation in China, South Korea and Japan. Several recent reports have shown that SF has anti-inflammatory and anti-apoptotic effects, indicating that it is a promising candidate for treatment of Parkinson’s disease (PD). We evaluated the protective effect of SF against neurotoxin 1-methyl-4-phenylpyridinium ion (MPP+)-induced mitochondrial dysfunction in SH-SY5Y human neuroblastoma cells, an in vitro PD model. SH-SY5Y cells were incubated with SF for 24 h, after which they were treated with MPP+. MPP+-induced cytotoxicity and apoptosis were confirmed by 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling assay. MitoSOX red mitochondrial superoxide indicator, tetramethylrhodamine methyl ester perchlorate and Parkin, PTEN-induced putative kinase 1 (PINK1), and DJ-1 immunofluorescent staining were conducted to confirm the mitochondrial function. In addition, western blot was performed to evaluate apoptosis factors (Bcl-2, Bax, caspase-3 and cytochrome c) and mitochondrial function-related factors (Parkin, PINK1 and DJ-1). SF suppressed MPP+-induced cytotoxicity, apoptosis and collapse of mitochondrial membrane potential by inhibiting the increase of reactive oxidative species (ROS) and DNA fragmentation, and controlling Bcl-2, Bax, caspase-3 and cytochrome c expression. Moreover, it attenuated Parkin, PINK1 and DJ-1 expression from MPP+-induced decrease. SF effectively suppressed MPP+-induced cytotoxicity, apoptosis and mitochondrial dysfunction by regulating generation of ROS, disruption of mitochondrial membrane potential, mitochondria-dependent apoptosis and loss or mutation of mitochondria-related PD markers including Parkin, PINK1 and DJ-1. |
| first_indexed | 2024-04-11T23:44:44Z |
| format | Article |
| id | doaj.art-bbdea7f5ebdd4ba78b64f38d94decf10 |
| institution | Directory Open Access Journal |
| issn | 1663-4365 |
| language | English |
| last_indexed | 2024-04-11T23:44:44Z |
| publishDate | 2018-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Aging Neuroscience |
| spelling | doaj.art-bbdea7f5ebdd4ba78b64f38d94decf102022-12-22T03:56:42ZengFrontiers Media S.A.Frontiers in Aging Neuroscience1663-43652018-04-011010.3389/fnagi.2018.00119348486Sophora flavescens Aiton Decreases MPP+-Induced Mitochondrial Dysfunction in SH-SY5Y CellsHee-Young Kim0Hyongjun Jeon1Hyungwoo Kim2Sungtae Koo3Sungtae Koo4Seungtae Kim5Seungtae Kim6Korean Medicine Research Center for Healthy Aging, Pusan National University, Yangsan, South KoreaDepartment of Korean Medical Science, School of Korean Medicine, Pusan National University, Yangsan, South KoreaDivision of Pharmacology, School of Korean Medicine, Pusan National University, Yangsan, South KoreaKorean Medicine Research Center for Healthy Aging, Pusan National University, Yangsan, South KoreaDepartment of Korean Medical Science, School of Korean Medicine, Pusan National University, Yangsan, South KoreaKorean Medicine Research Center for Healthy Aging, Pusan National University, Yangsan, South KoreaDepartment of Korean Medical Science, School of Korean Medicine, Pusan National University, Yangsan, South KoreaSophora flavescens Aiton (SF) has been used to treat various diseases including fever and inflammation in China, South Korea and Japan. Several recent reports have shown that SF has anti-inflammatory and anti-apoptotic effects, indicating that it is a promising candidate for treatment of Parkinson’s disease (PD). We evaluated the protective effect of SF against neurotoxin 1-methyl-4-phenylpyridinium ion (MPP+)-induced mitochondrial dysfunction in SH-SY5Y human neuroblastoma cells, an in vitro PD model. SH-SY5Y cells were incubated with SF for 24 h, after which they were treated with MPP+. MPP+-induced cytotoxicity and apoptosis were confirmed by 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay and terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling assay. MitoSOX red mitochondrial superoxide indicator, tetramethylrhodamine methyl ester perchlorate and Parkin, PTEN-induced putative kinase 1 (PINK1), and DJ-1 immunofluorescent staining were conducted to confirm the mitochondrial function. In addition, western blot was performed to evaluate apoptosis factors (Bcl-2, Bax, caspase-3 and cytochrome c) and mitochondrial function-related factors (Parkin, PINK1 and DJ-1). SF suppressed MPP+-induced cytotoxicity, apoptosis and collapse of mitochondrial membrane potential by inhibiting the increase of reactive oxidative species (ROS) and DNA fragmentation, and controlling Bcl-2, Bax, caspase-3 and cytochrome c expression. Moreover, it attenuated Parkin, PINK1 and DJ-1 expression from MPP+-induced decrease. SF effectively suppressed MPP+-induced cytotoxicity, apoptosis and mitochondrial dysfunction by regulating generation of ROS, disruption of mitochondrial membrane potential, mitochondria-dependent apoptosis and loss or mutation of mitochondria-related PD markers including Parkin, PINK1 and DJ-1.http://journal.frontiersin.org/article/10.3389/fnagi.2018.00119/fullParkinson’s diseaseSophora flavescens AitonSH-SY5YMPP+mitochondrial dysfunction |
| spellingShingle | Hee-Young Kim Hyongjun Jeon Hyungwoo Kim Sungtae Koo Sungtae Koo Seungtae Kim Seungtae Kim Sophora flavescens Aiton Decreases MPP+-Induced Mitochondrial Dysfunction in SH-SY5Y Cells Frontiers in Aging Neuroscience Parkinson’s disease Sophora flavescens Aiton SH-SY5Y MPP+ mitochondrial dysfunction |
| title | Sophora flavescens Aiton Decreases MPP+-Induced Mitochondrial Dysfunction in SH-SY5Y Cells |
| title_full | Sophora flavescens Aiton Decreases MPP+-Induced Mitochondrial Dysfunction in SH-SY5Y Cells |
| title_fullStr | Sophora flavescens Aiton Decreases MPP+-Induced Mitochondrial Dysfunction in SH-SY5Y Cells |
| title_full_unstemmed | Sophora flavescens Aiton Decreases MPP+-Induced Mitochondrial Dysfunction in SH-SY5Y Cells |
| title_short | Sophora flavescens Aiton Decreases MPP+-Induced Mitochondrial Dysfunction in SH-SY5Y Cells |
| title_sort | sophora flavescens aiton decreases mpp induced mitochondrial dysfunction in sh sy5y cells |
| topic | Parkinson’s disease Sophora flavescens Aiton SH-SY5Y MPP+ mitochondrial dysfunction |
| url | http://journal.frontiersin.org/article/10.3389/fnagi.2018.00119/full |
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