Transcriptomic Analysis of Differentially Expressed Genes in <i>Arabidopsis thaliana</i> Overexpressing <i>BnMYB2</i> from <i>Boehmeria nivea</i> under Cadmium Stress
<i>Boehmeria nivea</i> (ramie) is an important fiber crop with strong tolerance to cadmium (Cd). In our previous study, a novel MYB transcription factor gene from ramie, <i>BnMYB2</i>, was found to positively regulate Cd tolerance and accumulation in the transgenic <i>A...
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MDPI AG
2023-03-01
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| author | Shoujing Zhu Wenyang Li Suhui Yan Wenjuan Shi |
| author_facet | Shoujing Zhu Wenyang Li Suhui Yan Wenjuan Shi |
| author_sort | Shoujing Zhu |
| collection | DOAJ |
| description | <i>Boehmeria nivea</i> (ramie) is an important fiber crop with strong tolerance to cadmium (Cd). In our previous study, a novel MYB transcription factor gene from ramie, <i>BnMYB2</i>, was found to positively regulate Cd tolerance and accumulation in the transgenic <i>Arabidopsis thaliana</i> lines. Herein, transcriptome sequencing was performed to identify the differentially expressed genes involved in cadmium response between the wild-type (WT) and <i>BnMYB2</i> overexpressed lines; 1598 differentially expressed genes (DEGs) were detected in the shoot. GO and KEGG analysis indicated that the majority of DEGs belonged to the categories of transcription factors, plant hormone signal transduction and nitrogen metabolism. The expression level of the Ib subgroup <i>bHLH</i> genes (<i>AtbHLH38</i>, <i>AtbHLH39</i>, <i>AtbHLH100</i> and <i>AtbHLH101</i>) and nitrogen assimilation-related genes (<i>AtNIA1</i>, <i>AtNIA2</i>, <i>AtNIR1</i> and <i>AtASN2</i>) were significantly higher than that of WT, accompanied with the positive changes in iron (Fe) and total nitrogen content in the shoot of <i>BnMYB2</i> overexpression lines. Several DEGs belonging to the bZIP transcription factor family or SAUR family were also found up-regulated in the transgenic plants. These results provide important clues for elucidating how the molecular mechanisms of <i>BnMYB2</i> regulate plant response to Cd stress. |
| first_indexed | 2024-03-11T05:09:25Z |
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| id | doaj.art-bbdec11f38dc46dab49e5fefd1e33dc9 |
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| issn | 2073-4344 |
| language | English |
| last_indexed | 2024-03-11T05:09:25Z |
| publishDate | 2023-03-01 |
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| spelling | doaj.art-bbdec11f38dc46dab49e5fefd1e33dc92023-11-17T18:40:48ZengMDPI AGCatalysts2073-43442023-03-0113466210.3390/catal13040662Transcriptomic Analysis of Differentially Expressed Genes in <i>Arabidopsis thaliana</i> Overexpressing <i>BnMYB2</i> from <i>Boehmeria nivea</i> under Cadmium StressShoujing Zhu0Wenyang Li1Suhui Yan2Wenjuan Shi3College of Agriculture, Anhui Science and Technology University, Fengyang 233100, ChinaCollege of Agriculture, Anhui Science and Technology University, Fengyang 233100, ChinaCollege of Agriculture, Anhui Science and Technology University, Fengyang 233100, ChinaCollege of Agriculture, Anhui Science and Technology University, Fengyang 233100, China<i>Boehmeria nivea</i> (ramie) is an important fiber crop with strong tolerance to cadmium (Cd). In our previous study, a novel MYB transcription factor gene from ramie, <i>BnMYB2</i>, was found to positively regulate Cd tolerance and accumulation in the transgenic <i>Arabidopsis thaliana</i> lines. Herein, transcriptome sequencing was performed to identify the differentially expressed genes involved in cadmium response between the wild-type (WT) and <i>BnMYB2</i> overexpressed lines; 1598 differentially expressed genes (DEGs) were detected in the shoot. GO and KEGG analysis indicated that the majority of DEGs belonged to the categories of transcription factors, plant hormone signal transduction and nitrogen metabolism. The expression level of the Ib subgroup <i>bHLH</i> genes (<i>AtbHLH38</i>, <i>AtbHLH39</i>, <i>AtbHLH100</i> and <i>AtbHLH101</i>) and nitrogen assimilation-related genes (<i>AtNIA1</i>, <i>AtNIA2</i>, <i>AtNIR1</i> and <i>AtASN2</i>) were significantly higher than that of WT, accompanied with the positive changes in iron (Fe) and total nitrogen content in the shoot of <i>BnMYB2</i> overexpression lines. Several DEGs belonging to the bZIP transcription factor family or SAUR family were also found up-regulated in the transgenic plants. These results provide important clues for elucidating how the molecular mechanisms of <i>BnMYB2</i> regulate plant response to Cd stress.https://www.mdpi.com/2073-4344/13/4/662<i>Boehmeria nivea</i>transcriptome<i>BnMYB2</i>cadmium stresscatalysis of iron absorptioncatalysis of nitrogen assimilation |
| spellingShingle | Shoujing Zhu Wenyang Li Suhui Yan Wenjuan Shi Transcriptomic Analysis of Differentially Expressed Genes in <i>Arabidopsis thaliana</i> Overexpressing <i>BnMYB2</i> from <i>Boehmeria nivea</i> under Cadmium Stress Catalysts <i>Boehmeria nivea</i> transcriptome <i>BnMYB2</i> cadmium stress catalysis of iron absorption catalysis of nitrogen assimilation |
| title | Transcriptomic Analysis of Differentially Expressed Genes in <i>Arabidopsis thaliana</i> Overexpressing <i>BnMYB2</i> from <i>Boehmeria nivea</i> under Cadmium Stress |
| title_full | Transcriptomic Analysis of Differentially Expressed Genes in <i>Arabidopsis thaliana</i> Overexpressing <i>BnMYB2</i> from <i>Boehmeria nivea</i> under Cadmium Stress |
| title_fullStr | Transcriptomic Analysis of Differentially Expressed Genes in <i>Arabidopsis thaliana</i> Overexpressing <i>BnMYB2</i> from <i>Boehmeria nivea</i> under Cadmium Stress |
| title_full_unstemmed | Transcriptomic Analysis of Differentially Expressed Genes in <i>Arabidopsis thaliana</i> Overexpressing <i>BnMYB2</i> from <i>Boehmeria nivea</i> under Cadmium Stress |
| title_short | Transcriptomic Analysis of Differentially Expressed Genes in <i>Arabidopsis thaliana</i> Overexpressing <i>BnMYB2</i> from <i>Boehmeria nivea</i> under Cadmium Stress |
| title_sort | transcriptomic analysis of differentially expressed genes in i arabidopsis thaliana i overexpressing i bnmyb2 i from i boehmeria nivea i under cadmium stress |
| topic | <i>Boehmeria nivea</i> transcriptome <i>BnMYB2</i> cadmium stress catalysis of iron absorption catalysis of nitrogen assimilation |
| url | https://www.mdpi.com/2073-4344/13/4/662 |
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