Antioxidant Responses of Ragweed Leaf Beetle Ophraella communa (Coleoptera: Chrysomelidae) Exposed to Thermal Stress
Ophraellacommuna LeSage is an effective biological control agent of common ragweed, Ambrosia artemisiifolia L., which competes with crops and causes allergic rhinitis and asthma. However, thermal stress negatively affects the developmental fitness and body size of this beetle. High temperatures caus...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-07-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2018.00808/full |
| _version_ | 1818971811110453248 |
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| author | Hongsong Chen Hongsong Chen Ghulam Sarwar Solangi Jianying Guo Fanghao Wan Zhongshi Zhou |
| author_facet | Hongsong Chen Hongsong Chen Ghulam Sarwar Solangi Jianying Guo Fanghao Wan Zhongshi Zhou |
| author_sort | Hongsong Chen |
| collection | DOAJ |
| description | Ophraellacommuna LeSage is an effective biological control agent of common ragweed, Ambrosia artemisiifolia L., which competes with crops and causes allergic rhinitis and asthma. However, thermal stress negatively affects the developmental fitness and body size of this beetle. High temperatures cause a variety of physiological stress responses in insects, which can cause oxidative damage. We investigated the total protein content and activity of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), and peroxidases (PODs) in O. communa adults when its different developmental stages were exposed to high temperatures (40, 42, and 44°C) for 3 h each day for 3, 5, 5, and 5 days, respectively (by stage), and a whole generation to high temperatures (40, 42, and 44°C) for 3 h each day. A control group was reared at 28 ± 2°C. Under short-term daily phasic high-temperature stress, total protein contents were close to the control as a whole; overall, SOD activities increased significantly, CAT activities were closer to or even higher than the control, POD activities increased at 40°C, decreased at 42 or 44°C; stage-specific response was also observed. Under long-term daily phasic high-temperature stress, total protein content increased significantly at 44°C, SOD activities increased at higher temperatures, decreased at 44°C; CAT activities of females increased at ≤42°C, and decreased at 44°C, CAT activities of males decreased significantly; POD activities of females increased at 40°C, decreased at ≥42°C, POD activities of males decreased at 44°C; and antioxidant enzymes activities in females were significantly higher than those in males. Antioxidative enzymes protect O. communa from oxidative damage caused by thermal stress. |
| first_indexed | 2024-12-20T14:58:18Z |
| format | Article |
| id | doaj.art-bbdf243d2511465d9a1d11db0fede299 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-20T14:58:18Z |
| publishDate | 2018-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-bbdf243d2511465d9a1d11db0fede2992022-12-21T19:36:44ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2018-07-01910.3389/fphys.2018.00808362979Antioxidant Responses of Ragweed Leaf Beetle Ophraella communa (Coleoptera: Chrysomelidae) Exposed to Thermal StressHongsong Chen0Hongsong Chen1Ghulam Sarwar Solangi2Jianying Guo3Fanghao Wan4Zhongshi Zhou5State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaGuangxi Key Laboratory for Biology of Crop Diseases and Insect Pests, Institute of Plant Protection, Guangxi Academy of Agricultural Sciences, Nanning, ChinaDepartment of Entomology, Sindh Agriculture University Subcampus, Umerkot, PakistanState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaOphraellacommuna LeSage is an effective biological control agent of common ragweed, Ambrosia artemisiifolia L., which competes with crops and causes allergic rhinitis and asthma. However, thermal stress negatively affects the developmental fitness and body size of this beetle. High temperatures cause a variety of physiological stress responses in insects, which can cause oxidative damage. We investigated the total protein content and activity of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), and peroxidases (PODs) in O. communa adults when its different developmental stages were exposed to high temperatures (40, 42, and 44°C) for 3 h each day for 3, 5, 5, and 5 days, respectively (by stage), and a whole generation to high temperatures (40, 42, and 44°C) for 3 h each day. A control group was reared at 28 ± 2°C. Under short-term daily phasic high-temperature stress, total protein contents were close to the control as a whole; overall, SOD activities increased significantly, CAT activities were closer to or even higher than the control, POD activities increased at 40°C, decreased at 42 or 44°C; stage-specific response was also observed. Under long-term daily phasic high-temperature stress, total protein content increased significantly at 44°C, SOD activities increased at higher temperatures, decreased at 44°C; CAT activities of females increased at ≤42°C, and decreased at 44°C, CAT activities of males decreased significantly; POD activities of females increased at 40°C, decreased at ≥42°C, POD activities of males decreased at 44°C; and antioxidant enzymes activities in females were significantly higher than those in males. Antioxidative enzymes protect O. communa from oxidative damage caused by thermal stress.https://www.frontiersin.org/article/10.3389/fphys.2018.00808/fullOphraella communathermal stresstotal proteinantioxidant enzymes activitybiological control |
| spellingShingle | Hongsong Chen Hongsong Chen Ghulam Sarwar Solangi Jianying Guo Fanghao Wan Zhongshi Zhou Antioxidant Responses of Ragweed Leaf Beetle Ophraella communa (Coleoptera: Chrysomelidae) Exposed to Thermal Stress Frontiers in Physiology Ophraella communa thermal stress total protein antioxidant enzymes activity biological control |
| title | Antioxidant Responses of Ragweed Leaf Beetle Ophraella communa (Coleoptera: Chrysomelidae) Exposed to Thermal Stress |
| title_full | Antioxidant Responses of Ragweed Leaf Beetle Ophraella communa (Coleoptera: Chrysomelidae) Exposed to Thermal Stress |
| title_fullStr | Antioxidant Responses of Ragweed Leaf Beetle Ophraella communa (Coleoptera: Chrysomelidae) Exposed to Thermal Stress |
| title_full_unstemmed | Antioxidant Responses of Ragweed Leaf Beetle Ophraella communa (Coleoptera: Chrysomelidae) Exposed to Thermal Stress |
| title_short | Antioxidant Responses of Ragweed Leaf Beetle Ophraella communa (Coleoptera: Chrysomelidae) Exposed to Thermal Stress |
| title_sort | antioxidant responses of ragweed leaf beetle ophraella communa coleoptera chrysomelidae exposed to thermal stress |
| topic | Ophraella communa thermal stress total protein antioxidant enzymes activity biological control |
| url | https://www.frontiersin.org/article/10.3389/fphys.2018.00808/full |
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