Hydrolysis of Ibuprofen Nitrile and Ibuprofen Amide and Deracemisation of Ibuprofen Using Nocardia corallina B-276
A novel application of whole cells of Nocardia corallina B-276 for the deracemisation of ibuprofen is reported. This microorganism successfully hydrolysed ibuprofen nitrile to ibuprofen amide, and ibuprofen amide to ibuprofen, using a suspension of cells in a potassium phosphate buffer solution (0.1...
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| Format: | Article |
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MDPI AG
2012-03-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/17/3/3148/ |
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| author | Myrna Solís-Oba Norberto Manjarrez Aida Solís Ricardo Lievano Herminia Inés Pérez |
| author_facet | Myrna Solís-Oba Norberto Manjarrez Aida Solís Ricardo Lievano Herminia Inés Pérez |
| author_sort | Myrna Solís-Oba |
| collection | DOAJ |
| description | A novel application of whole cells of Nocardia corallina B-276 for the deracemisation of ibuprofen is reported. This microorganism successfully hydrolysed ibuprofen nitrile to ibuprofen amide, and ibuprofen amide to ibuprofen, using a suspension of cells in a potassium phosphate buffer solution (0.1 M, pH = 7.0). These results can be explained by the presence of NHase and amidase enzymes, but the reactions are not enantioselective and low ee values were obtained. However, (R)-ibuprofen was isolated with >99% ee by a deracemisation process catalysed by N. corallina B-276. This is the first report of this kind of catalysis with this microorganism. |
| first_indexed | 2024-12-13T06:56:41Z |
| format | Article |
| id | doaj.art-bbe14da6513543aaa07cb015d061f78b |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-13T06:56:41Z |
| publishDate | 2012-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-bbe14da6513543aaa07cb015d061f78b2022-12-21T23:56:01ZengMDPI AGMolecules1420-30492012-03-011733148315410.3390/molecules17033148Hydrolysis of Ibuprofen Nitrile and Ibuprofen Amide and Deracemisation of Ibuprofen Using Nocardia corallina B-276Myrna Solís-ObaNorberto ManjarrezAida SolísRicardo LievanoHerminia Inés PérezA novel application of whole cells of Nocardia corallina B-276 for the deracemisation of ibuprofen is reported. This microorganism successfully hydrolysed ibuprofen nitrile to ibuprofen amide, and ibuprofen amide to ibuprofen, using a suspension of cells in a potassium phosphate buffer solution (0.1 M, pH = 7.0). These results can be explained by the presence of NHase and amidase enzymes, but the reactions are not enantioselective and low ee values were obtained. However, (R)-ibuprofen was isolated with >99% ee by a deracemisation process catalysed by N. corallina B-276. This is the first report of this kind of catalysis with this microorganism.http://www.mdpi.com/1420-3049/17/3/3148/Nocardia corallinaderacemisationibuprofennitrile hydrataseamidase |
| spellingShingle | Myrna Solís-Oba Norberto Manjarrez Aida Solís Ricardo Lievano Herminia Inés Pérez Hydrolysis of Ibuprofen Nitrile and Ibuprofen Amide and Deracemisation of Ibuprofen Using Nocardia corallina B-276 Molecules Nocardia corallina deracemisation ibuprofen nitrile hydratase amidase |
| title | Hydrolysis of Ibuprofen Nitrile and Ibuprofen Amide and Deracemisation of Ibuprofen Using Nocardia corallina B-276 |
| title_full | Hydrolysis of Ibuprofen Nitrile and Ibuprofen Amide and Deracemisation of Ibuprofen Using Nocardia corallina B-276 |
| title_fullStr | Hydrolysis of Ibuprofen Nitrile and Ibuprofen Amide and Deracemisation of Ibuprofen Using Nocardia corallina B-276 |
| title_full_unstemmed | Hydrolysis of Ibuprofen Nitrile and Ibuprofen Amide and Deracemisation of Ibuprofen Using Nocardia corallina B-276 |
| title_short | Hydrolysis of Ibuprofen Nitrile and Ibuprofen Amide and Deracemisation of Ibuprofen Using Nocardia corallina B-276 |
| title_sort | hydrolysis of ibuprofen nitrile and ibuprofen amide and deracemisation of ibuprofen using nocardia corallina b 276 |
| topic | Nocardia corallina deracemisation ibuprofen nitrile hydratase amidase |
| url | http://www.mdpi.com/1420-3049/17/3/3148/ |
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