Toxic detection in mine water based on proteomic analysis of lysosomal enzymes in
Objectives Lysosome is the cell-organelle which is commonly used as biomonitoring tool in environmental pollution. In this study, the lysosomal proteomic of the yeast Saccharomyces cerevisiae was analyzed for utilization in the detection of toxic substances in mine water samples. Methods This work i...
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| Format: | Article |
| Language: | English |
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Korean Society of Environmental Health and Toxicology
2014-11-01
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| Series: | Environmental Health and Toxicology |
| Subjects: | |
| Online Access: | http://www.e-eht.org/upload/pdf/eht-29-e2014019.pdf |
| _version_ | 1819094880337526784 |
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| author | Ngoc-Tu Nguyen Yang-Hoon Kim Seung Hyuck Bang Ji Hye Hong Soon Dong Kwon Jiho Min |
| author_facet | Ngoc-Tu Nguyen Yang-Hoon Kim Seung Hyuck Bang Ji Hye Hong Soon Dong Kwon Jiho Min |
| author_sort | Ngoc-Tu Nguyen |
| collection | DOAJ |
| description | Objectives Lysosome is the cell-organelle which is commonly used as biomonitoring tool in environmental pollution. In this study, the lysosomal proteomic of the yeast Saccharomyces cerevisiae was analyzed for utilization in the detection of toxic substances in mine water samples. Methods This work informs the expression of lysosomal proteomic in yeast in response with toxic chemicals, such as sodium meta-arsenite and tetracycline, for screening specific biomarkers. After that, a recombinant yeast contained this biomarker were constructed for toxic detection in pure toxic chemicals and mine water samples. Results Each chemical had an optimal dose at which the fluorescent protein intensity reached the peak. In the case of water samples, the yeast showed the response with sample 1, 3, 4, and 5; whereas there is no response with sample 2, 6, and 7. Conclusions The recombinant yeast showed a high ability of toxic detection in response with several chemicals such as heavy metals and pharmaceuticals. In the case of mine water samples, the response varied depending on the sample content. |
| first_indexed | 2024-12-21T23:34:26Z |
| format | Article |
| id | doaj.art-bbe3304ae98b466aa20f5761d5e80597 |
| institution | Directory Open Access Journal |
| issn | 2233-6567 |
| language | English |
| last_indexed | 2024-12-21T23:34:26Z |
| publishDate | 2014-11-01 |
| publisher | Korean Society of Environmental Health and Toxicology |
| record_format | Article |
| series | Environmental Health and Toxicology |
| spelling | doaj.art-bbe3304ae98b466aa20f5761d5e805972022-12-21T18:46:24ZengKorean Society of Environmental Health and ToxicologyEnvironmental Health and Toxicology2233-65672014-11-012910.5620/eht.e2014019705Toxic detection in mine water based on proteomic analysis of lysosomal enzymes inNgoc-Tu Nguyen0Yang-Hoon Kim1Seung Hyuck Bang2Ji Hye Hong3Soon Dong Kwon4Jiho Min5 Department of Bioprocess Engineering, Chonbuk National University, Jeonju, Korea Department of Microbiology, Chungbuk National University, Cheongju, Korea Department of Bioprocess Engineering, Chonbuk National University, Jeonju, Korea Mine Reclamation Corporation, Coal Center, Seoul, Korea Mine Reclamation Corporation, Coal Center, Seoul, Korea Department of Bioprocess Engineering, Chonbuk National University, Jeonju, KoreaObjectives Lysosome is the cell-organelle which is commonly used as biomonitoring tool in environmental pollution. In this study, the lysosomal proteomic of the yeast Saccharomyces cerevisiae was analyzed for utilization in the detection of toxic substances in mine water samples. Methods This work informs the expression of lysosomal proteomic in yeast in response with toxic chemicals, such as sodium meta-arsenite and tetracycline, for screening specific biomarkers. After that, a recombinant yeast contained this biomarker were constructed for toxic detection in pure toxic chemicals and mine water samples. Results Each chemical had an optimal dose at which the fluorescent protein intensity reached the peak. In the case of water samples, the yeast showed the response with sample 1, 3, 4, and 5; whereas there is no response with sample 2, 6, and 7. Conclusions The recombinant yeast showed a high ability of toxic detection in response with several chemicals such as heavy metals and pharmaceuticals. In the case of mine water samples, the response varied depending on the sample content.http://www.e-eht.org/upload/pdf/eht-29-e2014019.pdfHeavy metalsLysosomal proteomicLysosomal responseMine waterPharmaceuticals |
| spellingShingle | Ngoc-Tu Nguyen Yang-Hoon Kim Seung Hyuck Bang Ji Hye Hong Soon Dong Kwon Jiho Min Toxic detection in mine water based on proteomic analysis of lysosomal enzymes in Environmental Health and Toxicology Heavy metals Lysosomal proteomic Lysosomal response Mine water Pharmaceuticals |
| title | Toxic detection in mine water based on proteomic analysis of lysosomal enzymes in |
| title_full | Toxic detection in mine water based on proteomic analysis of lysosomal enzymes in |
| title_fullStr | Toxic detection in mine water based on proteomic analysis of lysosomal enzymes in |
| title_full_unstemmed | Toxic detection in mine water based on proteomic analysis of lysosomal enzymes in |
| title_short | Toxic detection in mine water based on proteomic analysis of lysosomal enzymes in |
| title_sort | toxic detection in mine water based on proteomic analysis of lysosomal enzymes in |
| topic | Heavy metals Lysosomal proteomic Lysosomal response Mine water Pharmaceuticals |
| url | http://www.e-eht.org/upload/pdf/eht-29-e2014019.pdf |
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