Novel Graphene Biosensor Based on the Functionalization of Multifunctional Nano-bovine Serum Albumin for the Highly Sensitive Detection of Cancer Biomarkers
Abstract A simple, convenient, and highly sensitive bio-interface for graphene field-effect transistors (GFETs) based on multifunctional nano-denatured bovine serum albumin (nano-dBSA) functionalization was developed to target cancer biomarkers. The novel graphene–protein bioelectronic interface was...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2019-03-01
|
| Series: | Nano-Micro Letters |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1007/s40820-019-0250-8 |
| _version_ | 1818173873645944832 |
|---|---|
| author | Lin Zhou Kun Wang Hao Sun Simin Zhao Xianfeng Chen Dahong Qian Hongju Mao Jianlong Zhao |
| author_facet | Lin Zhou Kun Wang Hao Sun Simin Zhao Xianfeng Chen Dahong Qian Hongju Mao Jianlong Zhao |
| author_sort | Lin Zhou |
| collection | DOAJ |
| description | Abstract A simple, convenient, and highly sensitive bio-interface for graphene field-effect transistors (GFETs) based on multifunctional nano-denatured bovine serum albumin (nano-dBSA) functionalization was developed to target cancer biomarkers. The novel graphene–protein bioelectronic interface was constructed by heating to denature native BSA on the graphene substrate surface. The formed nano-dBSA film served as the cross-linker to immobilize monoclonal antibody against carcinoembryonic antigen (anti-CEA mAb) on the graphene channel activated by EDC and Sulfo-NHS. The nano-dBSA film worked as a self-protecting layer of graphene to prevent surface contamination by lithographic processing. The improved GFET biosensor exhibited good specificity and high sensitivity toward the target at an ultralow concentration of 337.58 fg mL−1. The electrical detection of the binding of CEA followed the Hill model for ligand–receptor interaction, indicating the negative binding cooperativity between CEA and anti-CEA mAb with a dissociation constant of 6.82 × 10−10 M. The multifunctional nano-dBSA functionalization can confer a new function to graphene-like 2D nanomaterials and provide a promising bio-functionalization method for clinical application in biosensing, nanomedicine, and drug delivery. |
| first_indexed | 2024-12-11T19:35:26Z |
| format | Article |
| id | doaj.art-bbe43b8d8b1746f7a6eb07c1b25a26e9 |
| institution | Directory Open Access Journal |
| issn | 2311-6706 2150-5551 |
| language | English |
| last_indexed | 2024-12-11T19:35:26Z |
| publishDate | 2019-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Nano-Micro Letters |
| spelling | doaj.art-bbe43b8d8b1746f7a6eb07c1b25a26e92022-12-22T00:53:10ZengSpringerOpenNano-Micro Letters2311-67062150-55512019-03-0111111310.1007/s40820-019-0250-8Novel Graphene Biosensor Based on the Functionalization of Multifunctional Nano-bovine Serum Albumin for the Highly Sensitive Detection of Cancer BiomarkersLin Zhou0Kun Wang1Hao Sun2Simin Zhao3Xianfeng Chen4Dahong Qian5Hongju Mao6Jianlong Zhao7State Key Laboratory of Transducer Technology; Key Laboratory of Terahertz Solid-State Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of SciencesState Key Laboratory of Transducer Technology; Key Laboratory of Terahertz Solid-State Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of SciencesState Key Laboratory of Transducer Technology; Key Laboratory of Terahertz Solid-State Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of SciencesSchool of Biomedical Engineering, Shanghai Jiao Tong UniversitySchool of Electronic Engineering, Bangor UniversitySchool of Biomedical Engineering, Shanghai Jiao Tong UniversityState Key Laboratory of Transducer Technology; Key Laboratory of Terahertz Solid-State Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of SciencesState Key Laboratory of Transducer Technology; Key Laboratory of Terahertz Solid-State Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of SciencesAbstract A simple, convenient, and highly sensitive bio-interface for graphene field-effect transistors (GFETs) based on multifunctional nano-denatured bovine serum albumin (nano-dBSA) functionalization was developed to target cancer biomarkers. The novel graphene–protein bioelectronic interface was constructed by heating to denature native BSA on the graphene substrate surface. The formed nano-dBSA film served as the cross-linker to immobilize monoclonal antibody against carcinoembryonic antigen (anti-CEA mAb) on the graphene channel activated by EDC and Sulfo-NHS. The nano-dBSA film worked as a self-protecting layer of graphene to prevent surface contamination by lithographic processing. The improved GFET biosensor exhibited good specificity and high sensitivity toward the target at an ultralow concentration of 337.58 fg mL−1. The electrical detection of the binding of CEA followed the Hill model for ligand–receptor interaction, indicating the negative binding cooperativity between CEA and anti-CEA mAb with a dissociation constant of 6.82 × 10−10 M. The multifunctional nano-dBSA functionalization can confer a new function to graphene-like 2D nanomaterials and provide a promising bio-functionalization method for clinical application in biosensing, nanomedicine, and drug delivery.http://link.springer.com/article/10.1007/s40820-019-0250-8Bio-interfaceMultifunctional denatured BSAGFET biosensorCancer biomarker |
| spellingShingle | Lin Zhou Kun Wang Hao Sun Simin Zhao Xianfeng Chen Dahong Qian Hongju Mao Jianlong Zhao Novel Graphene Biosensor Based on the Functionalization of Multifunctional Nano-bovine Serum Albumin for the Highly Sensitive Detection of Cancer Biomarkers Nano-Micro Letters Bio-interface Multifunctional denatured BSA GFET biosensor Cancer biomarker |
| title | Novel Graphene Biosensor Based on the Functionalization of Multifunctional Nano-bovine Serum Albumin for the Highly Sensitive Detection of Cancer Biomarkers |
| title_full | Novel Graphene Biosensor Based on the Functionalization of Multifunctional Nano-bovine Serum Albumin for the Highly Sensitive Detection of Cancer Biomarkers |
| title_fullStr | Novel Graphene Biosensor Based on the Functionalization of Multifunctional Nano-bovine Serum Albumin for the Highly Sensitive Detection of Cancer Biomarkers |
| title_full_unstemmed | Novel Graphene Biosensor Based on the Functionalization of Multifunctional Nano-bovine Serum Albumin for the Highly Sensitive Detection of Cancer Biomarkers |
| title_short | Novel Graphene Biosensor Based on the Functionalization of Multifunctional Nano-bovine Serum Albumin for the Highly Sensitive Detection of Cancer Biomarkers |
| title_sort | novel graphene biosensor based on the functionalization of multifunctional nano bovine serum albumin for the highly sensitive detection of cancer biomarkers |
| topic | Bio-interface Multifunctional denatured BSA GFET biosensor Cancer biomarker |
| url | http://link.springer.com/article/10.1007/s40820-019-0250-8 |
| work_keys_str_mv | AT linzhou novelgraphenebiosensorbasedonthefunctionalizationofmultifunctionalnanobovineserumalbuminforthehighlysensitivedetectionofcancerbiomarkers AT kunwang novelgraphenebiosensorbasedonthefunctionalizationofmultifunctionalnanobovineserumalbuminforthehighlysensitivedetectionofcancerbiomarkers AT haosun novelgraphenebiosensorbasedonthefunctionalizationofmultifunctionalnanobovineserumalbuminforthehighlysensitivedetectionofcancerbiomarkers AT siminzhao novelgraphenebiosensorbasedonthefunctionalizationofmultifunctionalnanobovineserumalbuminforthehighlysensitivedetectionofcancerbiomarkers AT xianfengchen novelgraphenebiosensorbasedonthefunctionalizationofmultifunctionalnanobovineserumalbuminforthehighlysensitivedetectionofcancerbiomarkers AT dahongqian novelgraphenebiosensorbasedonthefunctionalizationofmultifunctionalnanobovineserumalbuminforthehighlysensitivedetectionofcancerbiomarkers AT hongjumao novelgraphenebiosensorbasedonthefunctionalizationofmultifunctionalnanobovineserumalbuminforthehighlysensitivedetectionofcancerbiomarkers AT jianlongzhao novelgraphenebiosensorbasedonthefunctionalizationofmultifunctionalnanobovineserumalbuminforthehighlysensitivedetectionofcancerbiomarkers |