Emergence of <i>mcr-3 carrying Escherichia coli</i> in Diseased Pigs in South Korea
We examined the prevalence and molecular characteristics of <i>mcr-3</i> carrying colistin-resistant <i>Escherichia coli</i> among cattle, pig, and chicken isolates in South Korea. Among a total of 185 colistin-resistant <i>E. coli</i> isolates determined in this...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/10/1538 |
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| author | Abraham Fikru Mechesso Dong Chan Moon Hee Young Kang Hyun-Ju Song Su-Jeong Kim Ji-Hyun Choi Mi Hyun Kim Seok Hyeon Na Ha-Young Kim Byeong Yeal Jung Soon-Seek Yoon Suk-Kyung Lim |
| author_facet | Abraham Fikru Mechesso Dong Chan Moon Hee Young Kang Hyun-Ju Song Su-Jeong Kim Ji-Hyun Choi Mi Hyun Kim Seok Hyeon Na Ha-Young Kim Byeong Yeal Jung Soon-Seek Yoon Suk-Kyung Lim |
| author_sort | Abraham Fikru Mechesso |
| collection | DOAJ |
| description | We examined the prevalence and molecular characteristics of <i>mcr-3</i> carrying colistin-resistant <i>Escherichia coli</i> among cattle, pig, and chicken isolates in South Korea. Among a total of 185 colistin-resistant <i>E. coli</i> isolates determined in this study (47 from cattle, 90 from pigs, and 48 from chicken), PCR amplification detected <i>mcr</i>-<i>3</i> genes in 17 isolates predominantly from diseased pigs. The <i>mcr</i>-<i>3</i> genes were characterized as <i>mcr-3.1</i> in 15 isolates and <i>mcr</i>-<i>3.5</i> in 2 isolates. The <i>mcr-3</i> gene was transferred to the <i>E. coli</i> J53 recipient strain from more than 50% of the <i>mcr</i>-<i>3</i>-carrying isolates. The <i>mcr</i>-<i>3.1</i> and <i>mcr</i>-<i>3.5</i> genes were identified predominantly in IncHI2 and IncP plasmids, respectively. Multi-locus sequence typing analysis revealed eight previously reported sequence types (ST), including ST1, ST10, and ST42. We identified isolates with similar pulsed-field gel electrophoresis patterns from diseased pigs in three farms. Besides, the isolates carried various virulence factors and demonstrated resistance to multiple antimicrobials, including β-lactams and quinolones. Further, the <i>mcr</i>-<i>3.5</i> encodes three amino acid substitutions compared with <i>mcr</i>-<i>3.1</i>. To the best of our knowledge, this is the first report of pathogenic <i>E. coli</i> carrying <i>mcr</i>-<i>3.5</i> in South Korea, which implies that <i>mcr</i>-<i>3</i> variants may have already been widely spread in the pig industry. |
| first_indexed | 2024-03-10T15:49:07Z |
| format | Article |
| id | doaj.art-bbe44bb9c58446cc9d1a775036959818 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T15:49:07Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-bbe44bb9c58446cc9d1a7750369598182023-11-20T16:12:06ZengMDPI AGMicroorganisms2076-26072020-10-01810153810.3390/microorganisms8101538Emergence of <i>mcr-3 carrying Escherichia coli</i> in Diseased Pigs in South KoreaAbraham Fikru Mechesso0Dong Chan Moon1Hee Young Kang2Hyun-Ju Song3Su-Jeong Kim4Ji-Hyun Choi5Mi Hyun Kim6Seok Hyeon Na7Ha-Young Kim8Byeong Yeal Jung9Soon-Seek Yoon10Suk-Kyung Lim11Bacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeksin 8-ro, Gimcheon-si, Gyeongsangbuk-do 39660, KoreaBacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeksin 8-ro, Gimcheon-si, Gyeongsangbuk-do 39660, KoreaBacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeksin 8-ro, Gimcheon-si, Gyeongsangbuk-do 39660, KoreaBacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeksin 8-ro, Gimcheon-si, Gyeongsangbuk-do 39660, KoreaBacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeksin 8-ro, Gimcheon-si, Gyeongsangbuk-do 39660, KoreaBacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeksin 8-ro, Gimcheon-si, Gyeongsangbuk-do 39660, KoreaBacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeksin 8-ro, Gimcheon-si, Gyeongsangbuk-do 39660, KoreaBacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeksin 8-ro, Gimcheon-si, Gyeongsangbuk-do 39660, KoreaBacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeksin 8-ro, Gimcheon-si, Gyeongsangbuk-do 39660, KoreaBacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeksin 8-ro, Gimcheon-si, Gyeongsangbuk-do 39660, KoreaBacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeksin 8-ro, Gimcheon-si, Gyeongsangbuk-do 39660, KoreaBacterial Disease Division, Animal and Plant Quarantine Agency, 177 Hyeksin 8-ro, Gimcheon-si, Gyeongsangbuk-do 39660, KoreaWe examined the prevalence and molecular characteristics of <i>mcr-3</i> carrying colistin-resistant <i>Escherichia coli</i> among cattle, pig, and chicken isolates in South Korea. Among a total of 185 colistin-resistant <i>E. coli</i> isolates determined in this study (47 from cattle, 90 from pigs, and 48 from chicken), PCR amplification detected <i>mcr</i>-<i>3</i> genes in 17 isolates predominantly from diseased pigs. The <i>mcr</i>-<i>3</i> genes were characterized as <i>mcr-3.1</i> in 15 isolates and <i>mcr</i>-<i>3.5</i> in 2 isolates. The <i>mcr-3</i> gene was transferred to the <i>E. coli</i> J53 recipient strain from more than 50% of the <i>mcr</i>-<i>3</i>-carrying isolates. The <i>mcr</i>-<i>3.1</i> and <i>mcr</i>-<i>3.5</i> genes were identified predominantly in IncHI2 and IncP plasmids, respectively. Multi-locus sequence typing analysis revealed eight previously reported sequence types (ST), including ST1, ST10, and ST42. We identified isolates with similar pulsed-field gel electrophoresis patterns from diseased pigs in three farms. Besides, the isolates carried various virulence factors and demonstrated resistance to multiple antimicrobials, including β-lactams and quinolones. Further, the <i>mcr</i>-<i>3.5</i> encodes three amino acid substitutions compared with <i>mcr</i>-<i>3.1</i>. To the best of our knowledge, this is the first report of pathogenic <i>E. coli</i> carrying <i>mcr</i>-<i>3.5</i> in South Korea, which implies that <i>mcr</i>-<i>3</i> variants may have already been widely spread in the pig industry.https://www.mdpi.com/2076-2607/8/10/1538colistin<i>Escherichia coli</i><i>mcr</i>-<i>3</i> geneplasmidresistance |
| spellingShingle | Abraham Fikru Mechesso Dong Chan Moon Hee Young Kang Hyun-Ju Song Su-Jeong Kim Ji-Hyun Choi Mi Hyun Kim Seok Hyeon Na Ha-Young Kim Byeong Yeal Jung Soon-Seek Yoon Suk-Kyung Lim Emergence of <i>mcr-3 carrying Escherichia coli</i> in Diseased Pigs in South Korea Microorganisms colistin <i>Escherichia coli</i> <i>mcr</i>-<i>3</i> gene plasmid resistance |
| title | Emergence of <i>mcr-3 carrying Escherichia coli</i> in Diseased Pigs in South Korea |
| title_full | Emergence of <i>mcr-3 carrying Escherichia coli</i> in Diseased Pigs in South Korea |
| title_fullStr | Emergence of <i>mcr-3 carrying Escherichia coli</i> in Diseased Pigs in South Korea |
| title_full_unstemmed | Emergence of <i>mcr-3 carrying Escherichia coli</i> in Diseased Pigs in South Korea |
| title_short | Emergence of <i>mcr-3 carrying Escherichia coli</i> in Diseased Pigs in South Korea |
| title_sort | emergence of i mcr 3 carrying escherichia coli i in diseased pigs in south korea |
| topic | colistin <i>Escherichia coli</i> <i>mcr</i>-<i>3</i> gene plasmid resistance |
| url | https://www.mdpi.com/2076-2607/8/10/1538 |
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