Effect of bengkoang (Pachyrhizus erosus) fiber extract on murine macrophage-like J774.1 cells and mouse peritoneal macrophages
Bengkoang (Pachyrhizus erosus (L) Urban) is an edible root vegetable containing fairly large amounts of carbohydrates and crude fibers. The purpose of this study is to evaluate the effects of the bengkoang fiber extract (BFE) on macrophages in vitro and in vivo. BEF was prepared by heat-extraction f...
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| Format: | Article |
| Language: | English |
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Elsevier
2013-04-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464612001892 |
| _version_ | 1818384307450806272 |
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| author | Ika Dyah Kumalasari Kosuke Nishi Eni Harmayani Sri Raharjo Takuya Sugahara |
| author_facet | Ika Dyah Kumalasari Kosuke Nishi Eni Harmayani Sri Raharjo Takuya Sugahara |
| author_sort | Ika Dyah Kumalasari |
| collection | DOAJ |
| description | Bengkoang (Pachyrhizus erosus (L) Urban) is an edible root vegetable containing fairly large amounts of carbohydrates and crude fibers. The purpose of this study is to evaluate the effects of the bengkoang fiber extract (BFE) on macrophages in vitro and in vivo. BEF was prepared by heat-extraction from the bengkoang fiber in distilled water at 121 °C for 20 min. BFE stimulated the phagocytotic activity of J774.1 cells. In addition, BFE significantly facilitated production of tumor necrosis factor (TNF)-α and interleukin (IL)-6 by J774.1 cells and mouse peritoneal macrophages. BFE also facilitated the gene expression levels of inducible nitric oxide synthase and cyclooxygenase-2. The phagocytotic activity of mouse peritoneal macrophages was significantly enhanced by oral administration of BFE to BALB/c mice for 7 days. Ex vivo analysis revealed that production and gene expression of TNF-α and IL-6 in peritoneal macrophages from the BFE-administered mice were significantly enhanced. These results demonstrated that BFE activates macrophages in vitro and in vivo. |
| first_indexed | 2024-12-14T03:20:11Z |
| format | Article |
| id | doaj.art-bbe63fd65b384301894753b1dd182f6b |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-14T03:20:11Z |
| publishDate | 2013-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-bbe63fd65b384301894753b1dd182f6b2022-12-21T23:19:03ZengElsevierJournal of Functional Foods1756-46462013-04-0152582589Effect of bengkoang (Pachyrhizus erosus) fiber extract on murine macrophage-like J774.1 cells and mouse peritoneal macrophagesIka Dyah Kumalasari0Kosuke Nishi1Eni Harmayani2Sri Raharjo3Takuya Sugahara4Faculty of Agriculture, Ehime University, Matsuyama, Ehime 790-8566, JapanFaculty of Agriculture, Ehime University, Matsuyama, Ehime 790-8566, JapanFaculty of Agricultural Technology, Gadjah Mada University, Bulaksumur, Yogyakarta 55281, IndonesiaFaculty of Agricultural Technology, Gadjah Mada University, Bulaksumur, Yogyakarta 55281, IndonesiaFaculty of Agriculture, Ehime University, Matsuyama, Ehime 790-8566, Japan; South Ehime Fisheries Research Center, Ehime University, Ainan, Ehime 789-4292, Japan; Corresponding author at: Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan. Tel./fax: +81 89 946 9863.Bengkoang (Pachyrhizus erosus (L) Urban) is an edible root vegetable containing fairly large amounts of carbohydrates and crude fibers. The purpose of this study is to evaluate the effects of the bengkoang fiber extract (BFE) on macrophages in vitro and in vivo. BEF was prepared by heat-extraction from the bengkoang fiber in distilled water at 121 °C for 20 min. BFE stimulated the phagocytotic activity of J774.1 cells. In addition, BFE significantly facilitated production of tumor necrosis factor (TNF)-α and interleukin (IL)-6 by J774.1 cells and mouse peritoneal macrophages. BFE also facilitated the gene expression levels of inducible nitric oxide synthase and cyclooxygenase-2. The phagocytotic activity of mouse peritoneal macrophages was significantly enhanced by oral administration of BFE to BALB/c mice for 7 days. Ex vivo analysis revealed that production and gene expression of TNF-α and IL-6 in peritoneal macrophages from the BFE-administered mice were significantly enhanced. These results demonstrated that BFE activates macrophages in vitro and in vivo.http://www.sciencedirect.com/science/article/pii/S1756464612001892BengkoangJ774.1 cellsPeritoneal macrophage cellsPhagocytosisCytokines |
| spellingShingle | Ika Dyah Kumalasari Kosuke Nishi Eni Harmayani Sri Raharjo Takuya Sugahara Effect of bengkoang (Pachyrhizus erosus) fiber extract on murine macrophage-like J774.1 cells and mouse peritoneal macrophages Journal of Functional Foods Bengkoang J774.1 cells Peritoneal macrophage cells Phagocytosis Cytokines |
| title | Effect of bengkoang (Pachyrhizus erosus) fiber extract on murine macrophage-like J774.1 cells and mouse peritoneal macrophages |
| title_full | Effect of bengkoang (Pachyrhizus erosus) fiber extract on murine macrophage-like J774.1 cells and mouse peritoneal macrophages |
| title_fullStr | Effect of bengkoang (Pachyrhizus erosus) fiber extract on murine macrophage-like J774.1 cells and mouse peritoneal macrophages |
| title_full_unstemmed | Effect of bengkoang (Pachyrhizus erosus) fiber extract on murine macrophage-like J774.1 cells and mouse peritoneal macrophages |
| title_short | Effect of bengkoang (Pachyrhizus erosus) fiber extract on murine macrophage-like J774.1 cells and mouse peritoneal macrophages |
| title_sort | effect of bengkoang pachyrhizus erosus fiber extract on murine macrophage like j774 1 cells and mouse peritoneal macrophages |
| topic | Bengkoang J774.1 cells Peritoneal macrophage cells Phagocytosis Cytokines |
| url | http://www.sciencedirect.com/science/article/pii/S1756464612001892 |
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