Analysis of Chemical Composition Differences of Pericarpium Citri Reticulatae 'Chachi' with Storage from 7 to 11 Years by Widely-Targeted Metabolomics Technology
Objective: To analyze the differences of chemical composition of Pericarpium Citri Reticulatae 'Chachi' under different aging years, and to provide the theoretical and scientific basis for the aging mechanism of Pericarpium Citri Reticulatae 'Chachi'. Methods: UPLC-MS/MS widely-t...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
The editorial department of Science and Technology of Food Industry
2023-11-01
|
| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022120214 |
| _version_ | 1797650366126358528 |
|---|---|
| author | Yunli CHEN Renliang YAN Xuehua LU Lisha LI Yamin ZHANG Xiaomei XU Rongqing XU Wenjin LIN |
| author_facet | Yunli CHEN Renliang YAN Xuehua LU Lisha LI Yamin ZHANG Xiaomei XU Rongqing XU Wenjin LIN |
| author_sort | Yunli CHEN |
| collection | DOAJ |
| description | Objective: To analyze the differences of chemical composition of Pericarpium Citri Reticulatae 'Chachi' under different aging years, and to provide the theoretical and scientific basis for the aging mechanism of Pericarpium Citri Reticulatae 'Chachi'. Methods: UPLC-MS/MS widely-targeted metabolomics technology was used to detect and analyze the chemical constituents of three kinds of Pericarpium Citri Reticulatae 'Chachi' aged seven years, nine years and eleven years. PCA and OPLS-DA statistical analysis methods were used to identify differential metabolites. Results: A total of 778 compounds of 27 categories were identified. Main components were flavonoids (121) and terpenoids (90). According to the VIP value greater than 1 and the P-value less than 0.05, 121 differential metabolites were screened, 65 differential metabolites of 22 categories between the 11-year aging group and 9-year aging group, 62 differential metabolites of 20 categories between the 11-year aging group and 7-year aging group, 32 differential metabolites of 14 categories between the 9-year aging group and 7-year aging group. The numbers and types of differential metabolites in Pericarpium Citri Reticulatae 'Chachi' tended to increase after aging for 7~11 years. There were differences in metabolites of Pericarpium Citri Reticulatae 'Chachi' under different aging years. With the increase of aging time, the aging time had great influence on flavonoids, terpenoids, amino acid and derivatives. The key pathways with the highest correlation with differential metabolites were: Monoterpenoid biosynthesis, flavone and flavonol biosynthesis, the metabolic pathways involved in amino acid and derivatives (alanine, aspartate and glutamate metabolism, glycine, serine and threonine metabolism, cysteine and methionine metabolism). Conclusion: Widely-targeted metabolomics technology can be used to identify and analyze the chemical components of Pericarpium Citri Reticulatae 'Chachi' with high efficiency. It has established important technical foundation for researching the aging mechanism of Pericarpium Citri Reticulatae 'Chachi'. |
| first_indexed | 2024-03-11T16:00:18Z |
| format | Article |
| id | doaj.art-bbe7afdaab494308a283861766356dc8 |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-03-11T16:00:18Z |
| publishDate | 2023-11-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-bbe7afdaab494308a283861766356dc82023-10-25T07:38:40ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-11-0144211910.13386/j.issn1002-0306.20221202142022120214-21Analysis of Chemical Composition Differences of Pericarpium Citri Reticulatae 'Chachi' with Storage from 7 to 11 Years by Widely-Targeted Metabolomics TechnologyYunli CHEN0Renliang YAN1Xuehua LU2Lisha LI3Yamin ZHANG4Xiaomei XU5Rongqing XU6Wenjin LIN7Fujian Key Laboratory of Medical Analysis, Fujian Academy of Medical Sciences, Fuzhou 350001, ChinaGuangdong Food and Drug Vocational College, Guangzhou 510000, ChinaFujian Key Laboratory of Medical Analysis, Fujian Academy of Medical Sciences, Fuzhou 350001, ChinaFujian Key Laboratory of Medical Analysis, Fujian Academy of Medical Sciences, Fuzhou 350001, ChinaFujian Key Laboratory of Medical Analysis, Fujian Academy of Medical Sciences, Fuzhou 350001, ChinaFujian Key Laboratory of Medical Analysis, Fujian Academy of Medical Sciences, Fuzhou 350001, ChinaFujian Key Laboratory of Medical Analysis, Fujian Academy of Medical Sciences, Fuzhou 350001, ChinaFujian Key Laboratory of Medical Analysis, Fujian Academy of Medical Sciences, Fuzhou 350001, ChinaObjective: To analyze the differences of chemical composition of Pericarpium Citri Reticulatae 'Chachi' under different aging years, and to provide the theoretical and scientific basis for the aging mechanism of Pericarpium Citri Reticulatae 'Chachi'. Methods: UPLC-MS/MS widely-targeted metabolomics technology was used to detect and analyze the chemical constituents of three kinds of Pericarpium Citri Reticulatae 'Chachi' aged seven years, nine years and eleven years. PCA and OPLS-DA statistical analysis methods were used to identify differential metabolites. Results: A total of 778 compounds of 27 categories were identified. Main components were flavonoids (121) and terpenoids (90). According to the VIP value greater than 1 and the P-value less than 0.05, 121 differential metabolites were screened, 65 differential metabolites of 22 categories between the 11-year aging group and 9-year aging group, 62 differential metabolites of 20 categories between the 11-year aging group and 7-year aging group, 32 differential metabolites of 14 categories between the 9-year aging group and 7-year aging group. The numbers and types of differential metabolites in Pericarpium Citri Reticulatae 'Chachi' tended to increase after aging for 7~11 years. There were differences in metabolites of Pericarpium Citri Reticulatae 'Chachi' under different aging years. With the increase of aging time, the aging time had great influence on flavonoids, terpenoids, amino acid and derivatives. The key pathways with the highest correlation with differential metabolites were: Monoterpenoid biosynthesis, flavone and flavonol biosynthesis, the metabolic pathways involved in amino acid and derivatives (alanine, aspartate and glutamate metabolism, glycine, serine and threonine metabolism, cysteine and methionine metabolism). Conclusion: Widely-targeted metabolomics technology can be used to identify and analyze the chemical components of Pericarpium Citri Reticulatae 'Chachi' with high efficiency. It has established important technical foundation for researching the aging mechanism of Pericarpium Citri Reticulatae 'Chachi'.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022120214pericarpium citri reticulatae 'chachi'widely-targeted metabolomicsuplc-ms/msagingdifferential metabolites |
| spellingShingle | Yunli CHEN Renliang YAN Xuehua LU Lisha LI Yamin ZHANG Xiaomei XU Rongqing XU Wenjin LIN Analysis of Chemical Composition Differences of Pericarpium Citri Reticulatae 'Chachi' with Storage from 7 to 11 Years by Widely-Targeted Metabolomics Technology Shipin gongye ke-ji pericarpium citri reticulatae 'chachi' widely-targeted metabolomics uplc-ms/ms aging differential metabolites |
| title | Analysis of Chemical Composition Differences of Pericarpium Citri Reticulatae 'Chachi' with Storage from 7 to 11 Years by Widely-Targeted Metabolomics Technology |
| title_full | Analysis of Chemical Composition Differences of Pericarpium Citri Reticulatae 'Chachi' with Storage from 7 to 11 Years by Widely-Targeted Metabolomics Technology |
| title_fullStr | Analysis of Chemical Composition Differences of Pericarpium Citri Reticulatae 'Chachi' with Storage from 7 to 11 Years by Widely-Targeted Metabolomics Technology |
| title_full_unstemmed | Analysis of Chemical Composition Differences of Pericarpium Citri Reticulatae 'Chachi' with Storage from 7 to 11 Years by Widely-Targeted Metabolomics Technology |
| title_short | Analysis of Chemical Composition Differences of Pericarpium Citri Reticulatae 'Chachi' with Storage from 7 to 11 Years by Widely-Targeted Metabolomics Technology |
| title_sort | analysis of chemical composition differences of pericarpium citri reticulatae chachi with storage from 7 to 11 years by widely targeted metabolomics technology |
| topic | pericarpium citri reticulatae 'chachi' widely-targeted metabolomics uplc-ms/ms aging differential metabolites |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022120214 |
| work_keys_str_mv | AT yunlichen analysisofchemicalcompositiondifferencesofpericarpiumcitrireticulataechachiwithstoragefrom7to11yearsbywidelytargetedmetabolomicstechnology AT renliangyan analysisofchemicalcompositiondifferencesofpericarpiumcitrireticulataechachiwithstoragefrom7to11yearsbywidelytargetedmetabolomicstechnology AT xuehualu analysisofchemicalcompositiondifferencesofpericarpiumcitrireticulataechachiwithstoragefrom7to11yearsbywidelytargetedmetabolomicstechnology AT lishali analysisofchemicalcompositiondifferencesofpericarpiumcitrireticulataechachiwithstoragefrom7to11yearsbywidelytargetedmetabolomicstechnology AT yaminzhang analysisofchemicalcompositiondifferencesofpericarpiumcitrireticulataechachiwithstoragefrom7to11yearsbywidelytargetedmetabolomicstechnology AT xiaomeixu analysisofchemicalcompositiondifferencesofpericarpiumcitrireticulataechachiwithstoragefrom7to11yearsbywidelytargetedmetabolomicstechnology AT rongqingxu analysisofchemicalcompositiondifferencesofpericarpiumcitrireticulataechachiwithstoragefrom7to11yearsbywidelytargetedmetabolomicstechnology AT wenjinlin analysisofchemicalcompositiondifferencesofpericarpiumcitrireticulataechachiwithstoragefrom7to11yearsbywidelytargetedmetabolomicstechnology |