Systematic Discovery of FBXW7-Binding Phosphodegrons Highlights Mitogen-Activated Protein Kinases as Important Regulators of Intracellular Protein Levels
A FBXW7 is an F-box E3 ubiquitin-ligase affecting cell growth by controlling protein degradation. Mechanistically, its effect on its substrates depends on the phosphorylation of degron motifs, but the abundance of these phosphodegrons has not been systematically explored. We used a ratiometric prote...
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MDPI AG
2022-03-01
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| author | Neha Singh András Zeke Attila Reményi |
| author_facet | Neha Singh András Zeke Attila Reményi |
| author_sort | Neha Singh |
| collection | DOAJ |
| description | A FBXW7 is an F-box E3 ubiquitin-ligase affecting cell growth by controlling protein degradation. Mechanistically, its effect on its substrates depends on the phosphorylation of degron motifs, but the abundance of these phosphodegrons has not been systematically explored. We used a ratiometric protein degradation assay geared towards the identification of FBXW7-binding degron motifs phosphorylated by mitogen-activated protein kinases (MAPKs). Most of the known FBXW7 targets are localized in the nucleus and function as transcription factors. Here, in addition to more transcription affecting factors (ETV5, KLF4, SP5, JAZF1, and ZMIZ1 CAMTA2), we identified phosphodegrons located in proteins involved in chromatin regulation (ARID4B, KMT2E, KMT2D, and KAT6B) or cytoskeletal regulation (MAP2, Myozenin-2, SMTL2, and AKAP11), and some other proteins with miscellaneous functions (EIF4G3, CDT1, and CCAR2). We show that the protein level of full-length ARID4B, ETV5, JAZF1, and ZMIZ1 are affected by different MAPKs since their FBXW7-mediated degradation was diminished in the presence of MAPK-specific inhibitors. Our results suggest that MAPK and FBXW7 partnership plays an important cellular role by directly affecting the level of key regulatory proteins. The data also suggest that the p38α-controlled phosphodegron in JAZF1 may be responsible for the pathological regulation of the cancer-related JAZF1-SUZ12 fusion construct implicated in endometrial stromal sarcoma. |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T19:41:42Z |
| publishDate | 2022-03-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-bbe7bc4d52e744c5b695d4c9886a60d72023-11-24T01:37:17ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-03-01236332010.3390/ijms23063320Systematic Discovery of FBXW7-Binding Phosphodegrons Highlights Mitogen-Activated Protein Kinases as Important Regulators of Intracellular Protein LevelsNeha Singh0András Zeke1Attila Reményi2Biomolecular Interactions Research Group, Research Center for Natural Sciences, Institute of Organic Chemistry, H-1117 Budapest, HungaryBiomolecular Interactions Research Group, Research Center for Natural Sciences, Institute of Organic Chemistry, H-1117 Budapest, HungaryBiomolecular Interactions Research Group, Research Center for Natural Sciences, Institute of Organic Chemistry, H-1117 Budapest, HungaryA FBXW7 is an F-box E3 ubiquitin-ligase affecting cell growth by controlling protein degradation. Mechanistically, its effect on its substrates depends on the phosphorylation of degron motifs, but the abundance of these phosphodegrons has not been systematically explored. We used a ratiometric protein degradation assay geared towards the identification of FBXW7-binding degron motifs phosphorylated by mitogen-activated protein kinases (MAPKs). Most of the known FBXW7 targets are localized in the nucleus and function as transcription factors. Here, in addition to more transcription affecting factors (ETV5, KLF4, SP5, JAZF1, and ZMIZ1 CAMTA2), we identified phosphodegrons located in proteins involved in chromatin regulation (ARID4B, KMT2E, KMT2D, and KAT6B) or cytoskeletal regulation (MAP2, Myozenin-2, SMTL2, and AKAP11), and some other proteins with miscellaneous functions (EIF4G3, CDT1, and CCAR2). We show that the protein level of full-length ARID4B, ETV5, JAZF1, and ZMIZ1 are affected by different MAPKs since their FBXW7-mediated degradation was diminished in the presence of MAPK-specific inhibitors. Our results suggest that MAPK and FBXW7 partnership plays an important cellular role by directly affecting the level of key regulatory proteins. The data also suggest that the p38α-controlled phosphodegron in JAZF1 may be responsible for the pathological regulation of the cancer-related JAZF1-SUZ12 fusion construct implicated in endometrial stromal sarcoma.https://www.mdpi.com/1422-0067/23/6/3320FBXW7/FBW7/CDC4ubiquitin ligaseMAP kinaseERKJNKp38 |
| spellingShingle | Neha Singh András Zeke Attila Reményi Systematic Discovery of FBXW7-Binding Phosphodegrons Highlights Mitogen-Activated Protein Kinases as Important Regulators of Intracellular Protein Levels International Journal of Molecular Sciences FBXW7/FBW7/CDC4 ubiquitin ligase MAP kinase ERK JNK p38 |
| title | Systematic Discovery of FBXW7-Binding Phosphodegrons Highlights Mitogen-Activated Protein Kinases as Important Regulators of Intracellular Protein Levels |
| title_full | Systematic Discovery of FBXW7-Binding Phosphodegrons Highlights Mitogen-Activated Protein Kinases as Important Regulators of Intracellular Protein Levels |
| title_fullStr | Systematic Discovery of FBXW7-Binding Phosphodegrons Highlights Mitogen-Activated Protein Kinases as Important Regulators of Intracellular Protein Levels |
| title_full_unstemmed | Systematic Discovery of FBXW7-Binding Phosphodegrons Highlights Mitogen-Activated Protein Kinases as Important Regulators of Intracellular Protein Levels |
| title_short | Systematic Discovery of FBXW7-Binding Phosphodegrons Highlights Mitogen-Activated Protein Kinases as Important Regulators of Intracellular Protein Levels |
| title_sort | systematic discovery of fbxw7 binding phosphodegrons highlights mitogen activated protein kinases as important regulators of intracellular protein levels |
| topic | FBXW7/FBW7/CDC4 ubiquitin ligase MAP kinase ERK JNK p38 |
| url | https://www.mdpi.com/1422-0067/23/6/3320 |
| work_keys_str_mv | AT nehasingh systematicdiscoveryoffbxw7bindingphosphodegronshighlightsmitogenactivatedproteinkinasesasimportantregulatorsofintracellularproteinlevels AT andraszeke systematicdiscoveryoffbxw7bindingphosphodegronshighlightsmitogenactivatedproteinkinasesasimportantregulatorsofintracellularproteinlevels AT attilaremenyi systematicdiscoveryoffbxw7bindingphosphodegronshighlightsmitogenactivatedproteinkinasesasimportantregulatorsofintracellularproteinlevels |