Design, Optimization and Application of Small Molecule Biosensor in Metabolic Engineering
The development of synthetic biology and metabolic engineering has painted a great future for the bio-based economy, including fuels, chemicals, and drugs produced from renewable feedstocks. With the rapid advance of genome-scale modeling, pathway assembling and genome engineering/editing, our abili...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-10-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2017.02012/full |
| _version_ | 1818603024242704384 |
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| author | Yang Liu Ye Liu Meng Wang |
| author_facet | Yang Liu Ye Liu Meng Wang |
| author_sort | Yang Liu |
| collection | DOAJ |
| description | The development of synthetic biology and metabolic engineering has painted a great future for the bio-based economy, including fuels, chemicals, and drugs produced from renewable feedstocks. With the rapid advance of genome-scale modeling, pathway assembling and genome engineering/editing, our ability to design and generate microbial cell factories with various phenotype becomes almost limitless. However, our lack of ability to measure and exert precise control over metabolite concentration related phenotypes becomes a bottleneck in metabolic engineering. Genetically encoded small molecule biosensors, which provide the means to couple metabolite concentration to measurable or actionable outputs, are highly promising solutions to the bottleneck. Here we review recent advances in the design, optimization and application of small molecule biosensor in metabolic engineering, with particular focus on optimization strategies for transcription factor (TF) based biosensors. |
| first_indexed | 2024-12-16T13:16:35Z |
| format | Article |
| id | doaj.art-bbe8758fc21143998f85ce2335388796 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-16T13:16:35Z |
| publishDate | 2017-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-bbe8758fc21143998f85ce23353887962022-12-21T22:30:27ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2017-10-01810.3389/fmicb.2017.02012299351Design, Optimization and Application of Small Molecule Biosensor in Metabolic EngineeringYang LiuYe LiuMeng WangThe development of synthetic biology and metabolic engineering has painted a great future for the bio-based economy, including fuels, chemicals, and drugs produced from renewable feedstocks. With the rapid advance of genome-scale modeling, pathway assembling and genome engineering/editing, our ability to design and generate microbial cell factories with various phenotype becomes almost limitless. However, our lack of ability to measure and exert precise control over metabolite concentration related phenotypes becomes a bottleneck in metabolic engineering. Genetically encoded small molecule biosensors, which provide the means to couple metabolite concentration to measurable or actionable outputs, are highly promising solutions to the bottleneck. Here we review recent advances in the design, optimization and application of small molecule biosensor in metabolic engineering, with particular focus on optimization strategies for transcription factor (TF) based biosensors.http://journal.frontiersin.org/article/10.3389/fmicb.2017.02012/fullmetabolic engineeringsynthetic biologysmall molecule biosensortranscription factoroptimization strategyindustrial application |
| spellingShingle | Yang Liu Ye Liu Meng Wang Design, Optimization and Application of Small Molecule Biosensor in Metabolic Engineering Frontiers in Microbiology metabolic engineering synthetic biology small molecule biosensor transcription factor optimization strategy industrial application |
| title | Design, Optimization and Application of Small Molecule Biosensor in Metabolic Engineering |
| title_full | Design, Optimization and Application of Small Molecule Biosensor in Metabolic Engineering |
| title_fullStr | Design, Optimization and Application of Small Molecule Biosensor in Metabolic Engineering |
| title_full_unstemmed | Design, Optimization and Application of Small Molecule Biosensor in Metabolic Engineering |
| title_short | Design, Optimization and Application of Small Molecule Biosensor in Metabolic Engineering |
| title_sort | design optimization and application of small molecule biosensor in metabolic engineering |
| topic | metabolic engineering synthetic biology small molecule biosensor transcription factor optimization strategy industrial application |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2017.02012/full |
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