Aflatoxin M1 contamination in grated parmesan cheese marketed in Rio de Janeiro - Brazil
This study aimed to determine the occurrence of AFM1 contamination in the samples of grated parmesan cheese marketed in the Metropolitan Region of Rio de Janeiro -Brazil. Thirty samples representing 10 major brands marketed in the region were analyzed by High Performance Liquid Chromatography with f...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2014-04-01
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| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200016&lng=en&tlng=en |
| _version_ | 1828155573981413376 |
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| author | Felipe Machado Trombete Izabela Miranda de Castro Alessandra da Silva Teixeira Tatiana Saldanha Marcelo Elias Fraga |
| author_facet | Felipe Machado Trombete Izabela Miranda de Castro Alessandra da Silva Teixeira Tatiana Saldanha Marcelo Elias Fraga |
| author_sort | Felipe Machado Trombete |
| collection | DOAJ |
| description | This study aimed to determine the occurrence of AFM1 contamination in the samples of grated parmesan cheese marketed in the Metropolitan Region of Rio de Janeiro -Brazil. Thirty samples representing 10 major brands marketed in the region were analyzed by High Performance Liquid Chromatography with fluorescence detection (HPLC-FLD) after purification with immunoaffinity column. The method showed recovery values within the range of 70-90%, with RSD lower than 15% and limits of detection and quantification below the maximum level allowed by the European Commission for the presence of AFM1 in cheeses. The mycotoxin was identified in 18 (60%) of the grated cheese samples tested. The highest value corresponded to 0.69 ± 0.02 µg/kg and the mean for all the analyzed samples was 0.16 µg/kg. All the samples were lower than the limit established by the Brazilian legislation (2.5 µg/kg) for AFM1 in cheeses in general. However, eight samples (26.7%) presented AFM1 levels above the tolerance limit of 0.25 µg/kg adopted by the European Commission. These results indicated that AFM1 levels in the grated cheese consumed in Rio de Janeiro -Brazil were relatively high and it could provide a potential hazard for the public health. |
| first_indexed | 2024-04-11T22:55:44Z |
| format | Article |
| id | doaj.art-bbe8d4948fbb44bf958707d0c92befee |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-04-11T22:55:44Z |
| publishDate | 2014-04-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-bbe8d4948fbb44bf958707d0c92befee2022-12-22T03:58:26ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242014-04-01572269273S1516-89132014000200016Aflatoxin M1 contamination in grated parmesan cheese marketed in Rio de Janeiro - BrazilFelipe Machado Trombete0Izabela Miranda de Castro1Alessandra da Silva Teixeira2Tatiana Saldanha3Marcelo Elias Fraga4Universidade Federal Rural do Rio de JaneiroEmbrapa Agroindústria de AlimentosEmbrapa Agroindústria de AlimentosUniversidade Federal Rural do Rio de JaneiroUniversidade Federal Rural do Rio de JaneiroThis study aimed to determine the occurrence of AFM1 contamination in the samples of grated parmesan cheese marketed in the Metropolitan Region of Rio de Janeiro -Brazil. Thirty samples representing 10 major brands marketed in the region were analyzed by High Performance Liquid Chromatography with fluorescence detection (HPLC-FLD) after purification with immunoaffinity column. The method showed recovery values within the range of 70-90%, with RSD lower than 15% and limits of detection and quantification below the maximum level allowed by the European Commission for the presence of AFM1 in cheeses. The mycotoxin was identified in 18 (60%) of the grated cheese samples tested. The highest value corresponded to 0.69 ± 0.02 µg/kg and the mean for all the analyzed samples was 0.16 µg/kg. All the samples were lower than the limit established by the Brazilian legislation (2.5 µg/kg) for AFM1 in cheeses in general. However, eight samples (26.7%) presented AFM1 levels above the tolerance limit of 0.25 µg/kg adopted by the European Commission. These results indicated that AFM1 levels in the grated cheese consumed in Rio de Janeiro -Brazil were relatively high and it could provide a potential hazard for the public health.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200016&lng=en&tlng=enmycotoxindairy productsHPLC-FLD |
| spellingShingle | Felipe Machado Trombete Izabela Miranda de Castro Alessandra da Silva Teixeira Tatiana Saldanha Marcelo Elias Fraga Aflatoxin M1 contamination in grated parmesan cheese marketed in Rio de Janeiro - Brazil Brazilian Archives of Biology and Technology mycotoxin dairy products HPLC-FLD |
| title | Aflatoxin M1 contamination in grated parmesan cheese marketed in Rio de Janeiro - Brazil |
| title_full | Aflatoxin M1 contamination in grated parmesan cheese marketed in Rio de Janeiro - Brazil |
| title_fullStr | Aflatoxin M1 contamination in grated parmesan cheese marketed in Rio de Janeiro - Brazil |
| title_full_unstemmed | Aflatoxin M1 contamination in grated parmesan cheese marketed in Rio de Janeiro - Brazil |
| title_short | Aflatoxin M1 contamination in grated parmesan cheese marketed in Rio de Janeiro - Brazil |
| title_sort | aflatoxin m1 contamination in grated parmesan cheese marketed in rio de janeiro brazil |
| topic | mycotoxin dairy products HPLC-FLD |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000200016&lng=en&tlng=en |
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