Platycodin D induces apoptosis via regulating MAPK pathway and promotes autophagy in colon cancer cell
Platycodin D (PD) is the main component of triterpene saponins found in Platycodi radix. In this study, we observed a decrease in cell viability, an increase in apoptotic bodies, and an increase in the rate of apoptosis. Also, we observed an increase in cleaved PARP and Bax, a decrease in Bcl-2, and...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Biomedicine & Pharmacotherapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332224000970 |
| _version_ | 1797291853764100096 |
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| author | So-Hee Han Jae-Han Lee Joong-Seok Woo Gi-Hwan Jung Soo-Hyun Jung Eun-Ji Han Young-Seok Park Byeong-Soo Kim Sang-Ki Kim Byung-Kwon Park Ji-Youn Jung |
| author_facet | So-Hee Han Jae-Han Lee Joong-Seok Woo Gi-Hwan Jung Soo-Hyun Jung Eun-Ji Han Young-Seok Park Byeong-Soo Kim Sang-Ki Kim Byung-Kwon Park Ji-Youn Jung |
| author_sort | So-Hee Han |
| collection | DOAJ |
| description | Platycodin D (PD) is the main component of triterpene saponins found in Platycodi radix. In this study, we observed a decrease in cell viability, an increase in apoptotic bodies, and an increase in the rate of apoptosis. Also, we observed an increase in cleaved PARP and Bax, a decrease in Bcl-2, and p-ERK, and an increase in p-p38 and p-JNK. Furthermore, a change in cell viability and the expression of p-p38, Bax, and Bcl-2 using the p38 inhibitor revealed a decrease in p-p38 and Bax and an increase in Bcl-2 in the inhibitor treatment group. In addition, we observed an increase in vacuole formation through morphological changes and an increase in acidic vesicular organelles (AVOs). We also observed an increase in the expression of beclin 1, LC 3-I, and -II. There was no significant decrease in cell viability in the group treated with 3-MA, but a decrease in cell viability was noted in the group treated with HCQ. HCQ treatment resulted in an increase in Bax and a decrease in Bcl-2. These findings reveal that in HT-29 colon cancer cells, PD induces apoptosis through the MAPK pathway, thereby exerting anticancer effects. Moreover, autophagy caused by PD inhibits apoptosis by protecting the cells. |
| first_indexed | 2024-03-07T19:43:45Z |
| format | Article |
| id | doaj.art-bbec3e4ebc894718b067accabce5eb1e |
| institution | Directory Open Access Journal |
| issn | 0753-3322 |
| language | English |
| last_indexed | 2024-03-07T19:43:45Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedicine & Pharmacotherapy |
| spelling | doaj.art-bbec3e4ebc894718b067accabce5eb1e2024-02-29T05:17:46ZengElsevierBiomedicine & Pharmacotherapy0753-33222024-03-01172116216Platycodin D induces apoptosis via regulating MAPK pathway and promotes autophagy in colon cancer cellSo-Hee Han0Jae-Han Lee1Joong-Seok Woo2Gi-Hwan Jung3Soo-Hyun Jung4Eun-Ji Han5Young-Seok Park6Byeong-Soo Kim7Sang-Ki Kim8Byung-Kwon Park9Ji-Youn Jung10Department of Companion and Laboratory Animal Science, Kongju National University, Yesan 32439, Republic of KoreaDepartment of Companion and Laboratory Animal Science, Kongju National University, Yesan 32439, Republic of KoreaDepartment of Companion and Laboratory Animal Science, Kongju National University, Yesan 32439, Republic of KoreaDepartment of Companion and Laboratory Animal Science, Kongju National University, Yesan 32439, Republic of KoreaDepartment of Companion and Laboratory Animal Science, Kongju National University, Yesan 32439, Republic of KoreaDepartment of Companion and Laboratory Animal Science, Kongju National University, Yesan 32439, Republic of KoreaDepartment of Companion and Laboratory Animal Science, Kongju National University, Yesan 32439, Republic of KoreaDepartment of Companion and Laboratory Animal Science, Kongju National University, Yesan 32439, Republic of KoreaDepartment of Companion and Laboratory Animal Science, Kongju National University, Yesan 32439, Republic of KoreaDepartment of Companion and Laboratory Animal Science, Kongju National University, Yesan 32439, Republic of KoreaDepartment of Companion and Laboratory Animal Science, Kongju National University, Yesan 32439, Republic of Korea; Research Institute for Natural Products, Kongju National University, Yesan, Republic of Korea; Correspondence to: Department of Companion and Laboratory Animal Science, Kongju National University, Yesan 340-702, Republic of Korea.Platycodin D (PD) is the main component of triterpene saponins found in Platycodi radix. In this study, we observed a decrease in cell viability, an increase in apoptotic bodies, and an increase in the rate of apoptosis. Also, we observed an increase in cleaved PARP and Bax, a decrease in Bcl-2, and p-ERK, and an increase in p-p38 and p-JNK. Furthermore, a change in cell viability and the expression of p-p38, Bax, and Bcl-2 using the p38 inhibitor revealed a decrease in p-p38 and Bax and an increase in Bcl-2 in the inhibitor treatment group. In addition, we observed an increase in vacuole formation through morphological changes and an increase in acidic vesicular organelles (AVOs). We also observed an increase in the expression of beclin 1, LC 3-I, and -II. There was no significant decrease in cell viability in the group treated with 3-MA, but a decrease in cell viability was noted in the group treated with HCQ. HCQ treatment resulted in an increase in Bax and a decrease in Bcl-2. These findings reveal that in HT-29 colon cancer cells, PD induces apoptosis through the MAPK pathway, thereby exerting anticancer effects. Moreover, autophagy caused by PD inhibits apoptosis by protecting the cells.http://www.sciencedirect.com/science/article/pii/S0753332224000970Platycodin DColon cancerApoptosisAutophagyMAPK pathway |
| spellingShingle | So-Hee Han Jae-Han Lee Joong-Seok Woo Gi-Hwan Jung Soo-Hyun Jung Eun-Ji Han Young-Seok Park Byeong-Soo Kim Sang-Ki Kim Byung-Kwon Park Ji-Youn Jung Platycodin D induces apoptosis via regulating MAPK pathway and promotes autophagy in colon cancer cell Biomedicine & Pharmacotherapy Platycodin D Colon cancer Apoptosis Autophagy MAPK pathway |
| title | Platycodin D induces apoptosis via regulating MAPK pathway and promotes autophagy in colon cancer cell |
| title_full | Platycodin D induces apoptosis via regulating MAPK pathway and promotes autophagy in colon cancer cell |
| title_fullStr | Platycodin D induces apoptosis via regulating MAPK pathway and promotes autophagy in colon cancer cell |
| title_full_unstemmed | Platycodin D induces apoptosis via regulating MAPK pathway and promotes autophagy in colon cancer cell |
| title_short | Platycodin D induces apoptosis via regulating MAPK pathway and promotes autophagy in colon cancer cell |
| title_sort | platycodin d induces apoptosis via regulating mapk pathway and promotes autophagy in colon cancer cell |
| topic | Platycodin D Colon cancer Apoptosis Autophagy MAPK pathway |
| url | http://www.sciencedirect.com/science/article/pii/S0753332224000970 |
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