Changes in Microbiota Composition during the Anaerobic Digestion of Macroalgae in a Three-Stage Bioreactor
The use of microalgae as a raw material for biogas production is promising. Macroalgae were mixed with cattle manure, wheat straw, and an inoculant from sewage sludge. Mixing macroalgae with co-substrates increased biogas and methane yield. The research was carried out using a three-stage bioreactor...
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MDPI AG
2024-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/12/1/109 |
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| author | Dovilė Vasiliauskienė Mantas Pranskevičius Regimantas Dauknys Jaunius Urbonavičius Juliana Lukša Vadym Burko Alvydas Zagorskis |
| author_facet | Dovilė Vasiliauskienė Mantas Pranskevičius Regimantas Dauknys Jaunius Urbonavičius Juliana Lukša Vadym Burko Alvydas Zagorskis |
| author_sort | Dovilė Vasiliauskienė |
| collection | DOAJ |
| description | The use of microalgae as a raw material for biogas production is promising. Macroalgae were mixed with cattle manure, wheat straw, and an inoculant from sewage sludge. Mixing macroalgae with co-substrates increased biogas and methane yield. The research was carried out using a three-stage bioreactor. During biogas production, the dynamics of the composition of the microbiota in the anaerobic chamber of the bioreactor was evaluated. The microbiota composition at different organic load rates (OLRs) of the bioreactor was evaluated. This study also demonstrated that in a three-stage bioreactor, a higher yield of methane in biogas was obtained compared to a single-stage bioreactor. It was found that the most active functional pathway of methane biosynthesis is PWY-6969, which proceeds via the TCA cycle V (2-oxoglutarate synthase). Microbiota composition and methane yield depended on added volatile solids (VS<sub>added</sub>). During the research, it was found that after reducing the ORL from 2.44 to 1.09 kg VS/d, the methane yield increased from 175.2 L CH<sub>4</sub>/kg VS<sub>added</sub> to 323.5 L CH<sub>4</sub>/kg VS<sub>added</sub>. |
| first_indexed | 2024-03-08T09:51:14Z |
| format | Article |
| id | doaj.art-bbee09a6b466410f9d54a89aeefbdb0b |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-08T09:51:14Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-bbee09a6b466410f9d54a89aeefbdb0b2024-01-29T14:06:20ZengMDPI AGMicroorganisms2076-26072024-01-0112110910.3390/microorganisms12010109Changes in Microbiota Composition during the Anaerobic Digestion of Macroalgae in a Three-Stage BioreactorDovilė Vasiliauskienė0Mantas Pranskevičius1Regimantas Dauknys2Jaunius Urbonavičius3Juliana Lukša4Vadym Burko5Alvydas Zagorskis6Department of Chemistry and Bioengineering, Vilnius Gediminas Technical University, Sauletekio av. 11, 10223 Vilnius, LithuaniaInstitute of Environmental Protection, Vilnius Gediminas Technical University, 10223 Vilnius, LithuaniaDepartment of Environmental Protection and Water Engineering, Vilnius Gediminas Technical University, 10223 Vilnius, LithuaniaDepartment of Chemistry and Bioengineering, Vilnius Gediminas Technical University, Sauletekio av. 11, 10223 Vilnius, LithuaniaDepartment of Chemistry and Bioengineering, Vilnius Gediminas Technical University, Sauletekio av. 11, 10223 Vilnius, LithuaniaDepartment of Primary Science Institute of Modern Technologies, Pryazovskyi State Technical University, 87555 Mariupol, UkraineInstitute of Environmental Protection, Vilnius Gediminas Technical University, 10223 Vilnius, LithuaniaThe use of microalgae as a raw material for biogas production is promising. Macroalgae were mixed with cattle manure, wheat straw, and an inoculant from sewage sludge. Mixing macroalgae with co-substrates increased biogas and methane yield. The research was carried out using a three-stage bioreactor. During biogas production, the dynamics of the composition of the microbiota in the anaerobic chamber of the bioreactor was evaluated. The microbiota composition at different organic load rates (OLRs) of the bioreactor was evaluated. This study also demonstrated that in a three-stage bioreactor, a higher yield of methane in biogas was obtained compared to a single-stage bioreactor. It was found that the most active functional pathway of methane biosynthesis is PWY-6969, which proceeds via the TCA cycle V (2-oxoglutarate synthase). Microbiota composition and methane yield depended on added volatile solids (VS<sub>added</sub>). During the research, it was found that after reducing the ORL from 2.44 to 1.09 kg VS/d, the methane yield increased from 175.2 L CH<sub>4</sub>/kg VS<sub>added</sub> to 323.5 L CH<sub>4</sub>/kg VS<sub>added</sub>.https://www.mdpi.com/2076-2607/12/1/109biogasmethanemacroalgaethree-stage bioreactormicrobiota |
| spellingShingle | Dovilė Vasiliauskienė Mantas Pranskevičius Regimantas Dauknys Jaunius Urbonavičius Juliana Lukša Vadym Burko Alvydas Zagorskis Changes in Microbiota Composition during the Anaerobic Digestion of Macroalgae in a Three-Stage Bioreactor Microorganisms biogas methane macroalgae three-stage bioreactor microbiota |
| title | Changes in Microbiota Composition during the Anaerobic Digestion of Macroalgae in a Three-Stage Bioreactor |
| title_full | Changes in Microbiota Composition during the Anaerobic Digestion of Macroalgae in a Three-Stage Bioreactor |
| title_fullStr | Changes in Microbiota Composition during the Anaerobic Digestion of Macroalgae in a Three-Stage Bioreactor |
| title_full_unstemmed | Changes in Microbiota Composition during the Anaerobic Digestion of Macroalgae in a Three-Stage Bioreactor |
| title_short | Changes in Microbiota Composition during the Anaerobic Digestion of Macroalgae in a Three-Stage Bioreactor |
| title_sort | changes in microbiota composition during the anaerobic digestion of macroalgae in a three stage bioreactor |
| topic | biogas methane macroalgae three-stage bioreactor microbiota |
| url | https://www.mdpi.com/2076-2607/12/1/109 |
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