A modified hydrogel production protocol to decrease cellular content
ABSTRACT Purpose: To analyze the cytotoxicity and cell in porcine-derived decellularized skin matrix. Methods: We analyzed the effect of multiple decellularization processes by histological analysis, DNA quantification, and flow cytometry. Subsequently, we analyzed the most appropriate hydrogel co...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2022-12-01
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| Series: | Acta Cirúrgica Brasileira |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022001000203&tlng=en |
| _version_ | 1798004256097173504 |
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| author | Gabriela Catão Diniz Braga Cristina Pires Camargo Martin Conrad Harmsen Aristides Tadeu Correia Sonia Souza Marilia Seelaender Viviane Araujo Nunes Jeniffer Farias dos Santos Elida Adalgisa Neri Iuri Cordeiro Valadão Luiz Felipe Pinho Moreira Rolf Gemperli |
| author_facet | Gabriela Catão Diniz Braga Cristina Pires Camargo Martin Conrad Harmsen Aristides Tadeu Correia Sonia Souza Marilia Seelaender Viviane Araujo Nunes Jeniffer Farias dos Santos Elida Adalgisa Neri Iuri Cordeiro Valadão Luiz Felipe Pinho Moreira Rolf Gemperli |
| author_sort | Gabriela Catão Diniz Braga |
| collection | DOAJ |
| description | ABSTRACT Purpose: To analyze the cytotoxicity and cell in porcine-derived decellularized skin matrix. Methods: We analyzed the effect of multiple decellularization processes by histological analysis, DNA quantification, and flow cytometry. Subsequently, we analyzed the most appropriate hydrogel concentration to minimize cytotoxicity on fibroblast culture and to maximize cell proliferation. Results: After the fourth decellularization, the DNA quantification showed the lowest DNA concentration (< 50 ng/mg). Histological analysis showed no cell components in the hydrogel. Moreover, hematoxylin and eosin showed a heterogeneous structure of collagen fibers. The best hydrogel concentration ranged from 3 to 25%, and there was no significant difference between the 24 hours and seven days. Conclusions: The process of hydrogel production was effective for removing cells and DNA elements. The best hydrogel concentration ranged from 3 to 25%. |
| first_indexed | 2024-04-11T12:20:47Z |
| format | Article |
| id | doaj.art-bbefb931068f49aabbe0307bafda34c8 |
| institution | Directory Open Access Journal |
| issn | 1678-2674 |
| language | English |
| last_indexed | 2024-04-11T12:20:47Z |
| publishDate | 2022-12-01 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | Article |
| series | Acta Cirúrgica Brasileira |
| spelling | doaj.art-bbefb931068f49aabbe0307bafda34c82022-12-22T04:24:06ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira1678-26742022-12-01371010.1590/acb371005A modified hydrogel production protocol to decrease cellular contentGabriela Catão Diniz Bragahttps://orcid.org/0000-0003-2234-7702Cristina Pires Camargohttps://orcid.org/0000-0002-3134-0003Martin Conrad Harmsenhttps://orcid.org/0000-0002-7128-2741Aristides Tadeu Correiahttps://orcid.org/0000-0002-1195-6732Sonia Souzahttps://orcid.org/0000-0001-6981-9039Marilia Seelaenderhttps://orcid.org/0000-0002-9999-8020Viviane Araujo Nuneshttps://orcid.org/0000-0003-0861-4392Jeniffer Farias dos Santoshttps://orcid.org/0000-0002-2084-752XElida Adalgisa Nerihttps://orcid.org/0000-0003-1121-5413Iuri Cordeiro Valadãohttps://orcid.org/0000-0001-8417-6836Luiz Felipe Pinho Moreirahttps://orcid.org/0000-0003-0179-4976Rolf Gemperlihttps://orcid.org/0000-0002-4147-4478ABSTRACT Purpose: To analyze the cytotoxicity and cell in porcine-derived decellularized skin matrix. Methods: We analyzed the effect of multiple decellularization processes by histological analysis, DNA quantification, and flow cytometry. Subsequently, we analyzed the most appropriate hydrogel concentration to minimize cytotoxicity on fibroblast culture and to maximize cell proliferation. Results: After the fourth decellularization, the DNA quantification showed the lowest DNA concentration (< 50 ng/mg). Histological analysis showed no cell components in the hydrogel. Moreover, hematoxylin and eosin showed a heterogeneous structure of collagen fibers. The best hydrogel concentration ranged from 3 to 25%, and there was no significant difference between the 24 hours and seven days. Conclusions: The process of hydrogel production was effective for removing cells and DNA elements. The best hydrogel concentration ranged from 3 to 25%.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022001000203&tlng=enRegenerative MedicineBiocompatible MaterialsHydrogelsSkin, ArtificialCells, CulturedFibroblasts |
| spellingShingle | Gabriela Catão Diniz Braga Cristina Pires Camargo Martin Conrad Harmsen Aristides Tadeu Correia Sonia Souza Marilia Seelaender Viviane Araujo Nunes Jeniffer Farias dos Santos Elida Adalgisa Neri Iuri Cordeiro Valadão Luiz Felipe Pinho Moreira Rolf Gemperli A modified hydrogel production protocol to decrease cellular content Acta Cirúrgica Brasileira Regenerative Medicine Biocompatible Materials Hydrogels Skin, Artificial Cells, Cultured Fibroblasts |
| title | A modified hydrogel production protocol to decrease cellular content |
| title_full | A modified hydrogel production protocol to decrease cellular content |
| title_fullStr | A modified hydrogel production protocol to decrease cellular content |
| title_full_unstemmed | A modified hydrogel production protocol to decrease cellular content |
| title_short | A modified hydrogel production protocol to decrease cellular content |
| title_sort | modified hydrogel production protocol to decrease cellular content |
| topic | Regenerative Medicine Biocompatible Materials Hydrogels Skin, Artificial Cells, Cultured Fibroblasts |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022001000203&tlng=en |
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