Noradrenaline enhances the excitatory effects of dopamine on medial prefrontal cortex pyramidal neurons in rats
Abstract Aim Our previous studies showed that exposure to acute restraint stress enhanced cocaine‐induced conditioned place preference (cocaine‐CPP) and suggested the possibility that co‐activation of adrenergic transmission boosts the increase in medial prefrontal cortex (mPFC) neuronal activity by...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-12-01
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| Series: | Neuropsychopharmacology Reports |
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| Online Access: | https://doi.org/10.1002/npr2.12135 |
| _version_ | 1811185632085540864 |
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| author | Fumiya Shinohara Saya Arakaki Taiju Amano Masabumi Minami Katsuyuki Kaneda |
| author_facet | Fumiya Shinohara Saya Arakaki Taiju Amano Masabumi Minami Katsuyuki Kaneda |
| author_sort | Fumiya Shinohara |
| collection | DOAJ |
| description | Abstract Aim Our previous studies showed that exposure to acute restraint stress enhanced cocaine‐induced conditioned place preference (cocaine‐CPP) and suggested the possibility that co‐activation of adrenergic transmission boosts the increase in medial prefrontal cortex (mPFC) neuronal activity by the activation of dopaminergic transmission. To examine this possibility, the effects of the co‐treatment with dopamine (DA) and noradrenaline (NA) on mPFC neurons were compared with those of treatment with DA alone using whole‐cell patch‐clamp recordings. Methods The effects of DA alone and a mixture of DA and NA on the membrane potentials and spontaneous excitatory postsynaptic currents (sEPSCs) were examined by electrophysiological recordings of mPFC pyramidal neurons in brain slices of male Sprague Dawley rats. Extracellular DA and NA levels in the mPFC during and after restraint stress exposure were also examined by in vivo microdialysis. Results Dopamine significantly produced depolarizing effects on mPFC neurons and tended to increase sEPSC frequency. Co‐administration of NA with DA produced stronger depolarizing effects and significantly increased sEPSC frequency. The findings suggest that the additional depolarizing effect of NA on DA‐responsive neurons, rather than the excitation of DA‐nonresponsive neurons by NA, contributes to the stronger effect of co‐treatment of NA with DA. Conclusion The present study suggests that NA released by restraint stress exposure cooperates with DA to stimulate DA‐responsive neurons in the mPFC, thereby causing the stress‐induced enhancement of cocaine‐CPP. |
| first_indexed | 2024-04-11T13:32:34Z |
| format | Article |
| id | doaj.art-bbf2b967563d4395ad9bc078a1976275 |
| institution | Directory Open Access Journal |
| issn | 2574-173X |
| language | English |
| last_indexed | 2024-04-11T13:32:34Z |
| publishDate | 2020-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neuropsychopharmacology Reports |
| spelling | doaj.art-bbf2b967563d4395ad9bc078a19762752022-12-22T04:21:44ZengWileyNeuropsychopharmacology Reports2574-173X2020-12-0140434835410.1002/npr2.12135Noradrenaline enhances the excitatory effects of dopamine on medial prefrontal cortex pyramidal neurons in ratsFumiya Shinohara0Saya Arakaki1Taiju Amano2Masabumi Minami3Katsuyuki Kaneda4Department of Pharmacology Graduate School of Pharmaceutical Sciences Hokkaido University Sapporo JapanDepartment of Pharmacology Graduate School of Pharmaceutical Sciences Hokkaido University Sapporo JapanDepartment of Pharmacology Graduate School of Pharmaceutical Sciences Hokkaido University Sapporo JapanDepartment of Pharmacology Graduate School of Pharmaceutical Sciences Hokkaido University Sapporo JapanLaboratory of Molecular Pharmacology Institute of Medical, Pharmaceutical and Health Sciences Kanazawa University Kanazawa JapanAbstract Aim Our previous studies showed that exposure to acute restraint stress enhanced cocaine‐induced conditioned place preference (cocaine‐CPP) and suggested the possibility that co‐activation of adrenergic transmission boosts the increase in medial prefrontal cortex (mPFC) neuronal activity by the activation of dopaminergic transmission. To examine this possibility, the effects of the co‐treatment with dopamine (DA) and noradrenaline (NA) on mPFC neurons were compared with those of treatment with DA alone using whole‐cell patch‐clamp recordings. Methods The effects of DA alone and a mixture of DA and NA on the membrane potentials and spontaneous excitatory postsynaptic currents (sEPSCs) were examined by electrophysiological recordings of mPFC pyramidal neurons in brain slices of male Sprague Dawley rats. Extracellular DA and NA levels in the mPFC during and after restraint stress exposure were also examined by in vivo microdialysis. Results Dopamine significantly produced depolarizing effects on mPFC neurons and tended to increase sEPSC frequency. Co‐administration of NA with DA produced stronger depolarizing effects and significantly increased sEPSC frequency. The findings suggest that the additional depolarizing effect of NA on DA‐responsive neurons, rather than the excitation of DA‐nonresponsive neurons by NA, contributes to the stronger effect of co‐treatment of NA with DA. Conclusion The present study suggests that NA released by restraint stress exposure cooperates with DA to stimulate DA‐responsive neurons in the mPFC, thereby causing the stress‐induced enhancement of cocaine‐CPP.https://doi.org/10.1002/npr2.12135cocainedopaminemedial prefrontal cortexnoradrenalinestress |
| spellingShingle | Fumiya Shinohara Saya Arakaki Taiju Amano Masabumi Minami Katsuyuki Kaneda Noradrenaline enhances the excitatory effects of dopamine on medial prefrontal cortex pyramidal neurons in rats Neuropsychopharmacology Reports cocaine dopamine medial prefrontal cortex noradrenaline stress |
| title | Noradrenaline enhances the excitatory effects of dopamine on medial prefrontal cortex pyramidal neurons in rats |
| title_full | Noradrenaline enhances the excitatory effects of dopamine on medial prefrontal cortex pyramidal neurons in rats |
| title_fullStr | Noradrenaline enhances the excitatory effects of dopamine on medial prefrontal cortex pyramidal neurons in rats |
| title_full_unstemmed | Noradrenaline enhances the excitatory effects of dopamine on medial prefrontal cortex pyramidal neurons in rats |
| title_short | Noradrenaline enhances the excitatory effects of dopamine on medial prefrontal cortex pyramidal neurons in rats |
| title_sort | noradrenaline enhances the excitatory effects of dopamine on medial prefrontal cortex pyramidal neurons in rats |
| topic | cocaine dopamine medial prefrontal cortex noradrenaline stress |
| url | https://doi.org/10.1002/npr2.12135 |
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