Cell surface detection of vimentin, ACE2 and SARS-CoV-2 Spike proteins reveals selective colocalization at primary cilia
Abstract The SARS-CoV-2 Spike protein mediates docking of the virus onto cells prior to viral invasion. Several cellular receptors facilitate SARS-CoV-2 Spike docking at the cell surface, of which ACE2 plays a key role in many cell types. The intermediate filament protein vimentin has been reported...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-11248-y |
| _version_ | 1818263920072196096 |
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| author | Vasiliki Lalioti Silvia González-Sanz Irene Lois-Bermejo Patricia González-Jiménez Álvaro Viedma-Poyatos Andrea Merino María A. Pajares Dolores Pérez-Sala |
| author_facet | Vasiliki Lalioti Silvia González-Sanz Irene Lois-Bermejo Patricia González-Jiménez Álvaro Viedma-Poyatos Andrea Merino María A. Pajares Dolores Pérez-Sala |
| author_sort | Vasiliki Lalioti |
| collection | DOAJ |
| description | Abstract The SARS-CoV-2 Spike protein mediates docking of the virus onto cells prior to viral invasion. Several cellular receptors facilitate SARS-CoV-2 Spike docking at the cell surface, of which ACE2 plays a key role in many cell types. The intermediate filament protein vimentin has been reported to be present at the surface of certain cells and act as a co-receptor for several viruses; furthermore, its potential involvement in interactions with Spike proteins has been proposed. Nevertheless, the potential colocalization of vimentin with Spike and its receptors on the cell surface has not been explored. Here we have assessed the binding of Spike protein constructs to several cell types. Incubation of cells with tagged Spike S or Spike S1 subunit led to discrete dotted patterns at the cell surface, which consistently colocalized with endogenous ACE2, but sparsely with a lipid raft marker. Vimentin immunoreactivity mostly appeared as spots or patches unevenly distributed at the surface of diverse cell types. Of note, vimentin could also be detected in extracellular particles and in the cytoplasm underlying areas of compromised plasma membrane. Interestingly, although overall colocalization of vimentin-positive spots with ACE2 or Spike was moderate, a selective enrichment of the three proteins was detected at elongated structures, positive for acetylated tubulin and ARL13B. These structures, consistent with primary cilia, concentrated Spike binding at the top of the cells. Our results suggest that a vimentin-Spike interaction could occur at selective locations of the cell surface, including ciliated structures, which can act as platforms for SARS-CoV-2 docking. |
| first_indexed | 2024-12-12T19:26:41Z |
| format | Article |
| id | doaj.art-bbf322cc1241404b9e9a889e1b6e17a6 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-12T19:26:41Z |
| publishDate | 2022-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-bbf322cc1241404b9e9a889e1b6e17a62022-12-22T00:14:30ZengNature PortfolioScientific Reports2045-23222022-04-0112111910.1038/s41598-022-11248-yCell surface detection of vimentin, ACE2 and SARS-CoV-2 Spike proteins reveals selective colocalization at primary ciliaVasiliki Lalioti0Silvia González-Sanz1Irene Lois-Bermejo2Patricia González-Jiménez3Álvaro Viedma-Poyatos4Andrea Merino5María A. Pajares6Dolores Pérez-Sala7Department of Structural and Chemical Biology, Centro de Investigaciones Biológicas Margarita Salas, CSICDepartment of Structural and Chemical Biology, Centro de Investigaciones Biológicas Margarita Salas, CSICDepartment of Structural and Chemical Biology, Centro de Investigaciones Biológicas Margarita Salas, CSICDepartment of Structural and Chemical Biology, Centro de Investigaciones Biológicas Margarita Salas, CSICDepartment of Structural and Chemical Biology, Centro de Investigaciones Biológicas Margarita Salas, CSICDepartment of Structural and Chemical Biology, Centro de Investigaciones Biológicas Margarita Salas, CSICDepartment of Structural and Chemical Biology, Centro de Investigaciones Biológicas Margarita Salas, CSICDepartment of Structural and Chemical Biology, Centro de Investigaciones Biológicas Margarita Salas, CSICAbstract The SARS-CoV-2 Spike protein mediates docking of the virus onto cells prior to viral invasion. Several cellular receptors facilitate SARS-CoV-2 Spike docking at the cell surface, of which ACE2 plays a key role in many cell types. The intermediate filament protein vimentin has been reported to be present at the surface of certain cells and act as a co-receptor for several viruses; furthermore, its potential involvement in interactions with Spike proteins has been proposed. Nevertheless, the potential colocalization of vimentin with Spike and its receptors on the cell surface has not been explored. Here we have assessed the binding of Spike protein constructs to several cell types. Incubation of cells with tagged Spike S or Spike S1 subunit led to discrete dotted patterns at the cell surface, which consistently colocalized with endogenous ACE2, but sparsely with a lipid raft marker. Vimentin immunoreactivity mostly appeared as spots or patches unevenly distributed at the surface of diverse cell types. Of note, vimentin could also be detected in extracellular particles and in the cytoplasm underlying areas of compromised plasma membrane. Interestingly, although overall colocalization of vimentin-positive spots with ACE2 or Spike was moderate, a selective enrichment of the three proteins was detected at elongated structures, positive for acetylated tubulin and ARL13B. These structures, consistent with primary cilia, concentrated Spike binding at the top of the cells. Our results suggest that a vimentin-Spike interaction could occur at selective locations of the cell surface, including ciliated structures, which can act as platforms for SARS-CoV-2 docking.https://doi.org/10.1038/s41598-022-11248-y |
| spellingShingle | Vasiliki Lalioti Silvia González-Sanz Irene Lois-Bermejo Patricia González-Jiménez Álvaro Viedma-Poyatos Andrea Merino María A. Pajares Dolores Pérez-Sala Cell surface detection of vimentin, ACE2 and SARS-CoV-2 Spike proteins reveals selective colocalization at primary cilia Scientific Reports |
| title | Cell surface detection of vimentin, ACE2 and SARS-CoV-2 Spike proteins reveals selective colocalization at primary cilia |
| title_full | Cell surface detection of vimentin, ACE2 and SARS-CoV-2 Spike proteins reveals selective colocalization at primary cilia |
| title_fullStr | Cell surface detection of vimentin, ACE2 and SARS-CoV-2 Spike proteins reveals selective colocalization at primary cilia |
| title_full_unstemmed | Cell surface detection of vimentin, ACE2 and SARS-CoV-2 Spike proteins reveals selective colocalization at primary cilia |
| title_short | Cell surface detection of vimentin, ACE2 and SARS-CoV-2 Spike proteins reveals selective colocalization at primary cilia |
| title_sort | cell surface detection of vimentin ace2 and sars cov 2 spike proteins reveals selective colocalization at primary cilia |
| url | https://doi.org/10.1038/s41598-022-11248-y |
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