Occurrence, Phenotypic and Molecular Characteristics of Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i> in Healthy Turkeys in Northern Egypt
Poultry is one of the most important reservoirs for zoonotic multidrug-resistant pathogens. The indiscriminate use of antimicrobials in poultry production is a leading factor for development and dissemination of antimicrobial resistance. This study aimed to describe the prevalence and antimicrobial...
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| 格式: | 文件 |
| 语言: | English |
| 出版: |
MDPI AG
2022-08-01
|
| 丛编: | Antibiotics |
| 主题: | |
| 在线阅读: | https://www.mdpi.com/2079-6382/11/8/1075 |
| _version_ | 1827601016052252672 |
|---|---|
| author | Amira A. Moawad Helmut Hotzel Hafez M. Hafez Hazem Ramadan Herbert Tomaso Sascha D. Braun Ralf Ehricht Celia Diezel Dominik Gary Ines Engelmann Islam M. Zakaria Reem M. Reda Samah Eid Momtaz A. Shahien Heinrich Neubauer Stefan Monecke |
| author_facet | Amira A. Moawad Helmut Hotzel Hafez M. Hafez Hazem Ramadan Herbert Tomaso Sascha D. Braun Ralf Ehricht Celia Diezel Dominik Gary Ines Engelmann Islam M. Zakaria Reem M. Reda Samah Eid Momtaz A. Shahien Heinrich Neubauer Stefan Monecke |
| author_sort | Amira A. Moawad |
| collection | DOAJ |
| description | Poultry is one of the most important reservoirs for zoonotic multidrug-resistant pathogens. The indiscriminate use of antimicrobials in poultry production is a leading factor for development and dissemination of antimicrobial resistance. This study aimed to describe the prevalence and antimicrobial resistance of <i>E. coli</i> isolated from healthy turkey flocks of different ages in Nile delta region, Egypt. In the current investigation, 250 cloacal swabs were collected from 12 turkey farms in five governorates in the northern Egypt. Collected samples were cultivated on Brilliance<sup>TM</sup> ESBL agar media supplemented with cefotaxime (100 mg/L). The <i>E. coli</i> isolates were identified using MALDI-TOF-MS and confirmed by a conventional PCR assay targeting 16S rRNA-DNA. The phenotypic antibiogram against 14 antimicrobial agents was determined using the broth micro-dilution method. DNA-microarray-based assay was applied for genotyping and determination of both, virulence and resistance-associated gene markers. Multiplex real-time PCR was additionally applied for all isolates for detection of the actual most relevant Carbapenemase genes. The phenotypic identification of colistin resistance was carried out using E-test. A total of 26 <i>E. coli</i> isolates were recovered from the cloacal samples. All isolates were defined as multidrug-resistant. Interestingly, two different <i>E. coli</i> strains were isolated from one sample. Both strains had different phenotypic and genotypic profiles. All isolates were phenotypically susceptible to imipenem, while resistant to penicillin, rifampicin, streptomycin, and erythromycin. None of the examined carbapenem resistance genes was detected among isolates. At least one beta-lactamase gene was identified in most of isolates, where <i>bla</i>TEM was the most commonly identified determinant (80.8%), in addition to <i>bla</i>CTX-M9 (23.1%), <i>bla</i>SHV (19.2%) and <i>bla</i>OXA-10 (15.4%). Genes associated with chloramphenicol resistance were <i>flo</i>R (65.4%) and <i>cml</i>A1 (46.2%). Tetracycline- and quinolone-resistance-associated genes <i>tet</i>A and <i>qnr</i>S were detected in (57.7%) and (50.0%) of isolates, respectively. The aminoglycoside resistance associated genes <i>aad</i>A1 (65.4%), <i>aad</i>A2 (53.8%), <i>aph</i>A (50.0%), <i>str</i>A (69.2%), and <i>str</i>B (65.4%), were detected among isolates. Macrolide resistance associated genes <i>mph</i> and <i>mrx</i> were also detected in (53.8%) and (34.6%). Moreover, colistin resistance associated gene <i>mcr</i>-9 was identified in one isolate (3.8%). The class 1 integron integrase <i>int</i>I1 (84.6%), transposase for the transposon <i>tnp</i>ISEcp1 (34.6%) and OqxB -integral membrane and component of RND-type multidrug efflux pump <i>oqx</i>B (7.7%) were identified among the isolates. The existing high incidence of ESBL/colistin-producing <i>E. coli</i> identified in healthy turkeys is a major concern that demands prompt control; otherwise, such strains and their resistance determinants could be transmitted to other bacteria and, eventually, to people via the food chain. |
| first_indexed | 2024-03-09T04:47:14Z |
| format | Article |
| id | doaj.art-bbfa9af4db874ef6ba1adcf5f97df51c |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T04:47:14Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-bbfa9af4db874ef6ba1adcf5f97df51c2023-12-03T13:14:41ZengMDPI AGAntibiotics2079-63822022-08-01118107510.3390/antibiotics11081075Occurrence, Phenotypic and Molecular Characteristics of Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i> in Healthy Turkeys in Northern EgyptAmira A. Moawad0Helmut Hotzel1Hafez M. Hafez2Hazem Ramadan3Herbert Tomaso4Sascha D. Braun5Ralf Ehricht6Celia Diezel7Dominik Gary8Ines Engelmann9Islam M. Zakaria10Reem M. Reda11Samah Eid12Momtaz A. Shahien13Heinrich Neubauer14Stefan Monecke15Friedrich-Loeffler-Institut, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, GermanyFriedrich-Loeffler-Institut, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, GermanyInstitute for Poultry Diseases, Free University Berlin, Königsweg 63, 14163 Berlin, GermanyHygiene and Zoonoses Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptFriedrich-Loeffler-Institut, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, GermanyLeibniz Institute of Photonic Technology (IPHT), 07745 Jena, GermanyLeibniz Institute of Photonic Technology (IPHT), 07745 Jena, GermanyLeibniz Institute of Photonic Technology (IPHT), 07745 Jena, GermanyINTER-ARRAY by fzmb GmbH, 99947 Bad Langensalza, GermanyBLINK AG, 07747 Jena, GermanyAnimal Health Research Institute, Agriculture Research Center (ARC), Giza 12618, EgyptAnimal Health Research Institute, Agriculture Research Center (ARC), Giza 12618, EgyptAnimal Health Research Institute, Agriculture Research Center (ARC), Giza 12618, EgyptAnimal Health Research Institute, Agriculture Research Center (ARC), Giza 12618, EgyptFriedrich-Loeffler-Institut, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, GermanyLeibniz Institute of Photonic Technology (IPHT), 07745 Jena, GermanyPoultry is one of the most important reservoirs for zoonotic multidrug-resistant pathogens. The indiscriminate use of antimicrobials in poultry production is a leading factor for development and dissemination of antimicrobial resistance. This study aimed to describe the prevalence and antimicrobial resistance of <i>E. coli</i> isolated from healthy turkey flocks of different ages in Nile delta region, Egypt. In the current investigation, 250 cloacal swabs were collected from 12 turkey farms in five governorates in the northern Egypt. Collected samples were cultivated on Brilliance<sup>TM</sup> ESBL agar media supplemented with cefotaxime (100 mg/L). The <i>E. coli</i> isolates were identified using MALDI-TOF-MS and confirmed by a conventional PCR assay targeting 16S rRNA-DNA. The phenotypic antibiogram against 14 antimicrobial agents was determined using the broth micro-dilution method. DNA-microarray-based assay was applied for genotyping and determination of both, virulence and resistance-associated gene markers. Multiplex real-time PCR was additionally applied for all isolates for detection of the actual most relevant Carbapenemase genes. The phenotypic identification of colistin resistance was carried out using E-test. A total of 26 <i>E. coli</i> isolates were recovered from the cloacal samples. All isolates were defined as multidrug-resistant. Interestingly, two different <i>E. coli</i> strains were isolated from one sample. Both strains had different phenotypic and genotypic profiles. All isolates were phenotypically susceptible to imipenem, while resistant to penicillin, rifampicin, streptomycin, and erythromycin. None of the examined carbapenem resistance genes was detected among isolates. At least one beta-lactamase gene was identified in most of isolates, where <i>bla</i>TEM was the most commonly identified determinant (80.8%), in addition to <i>bla</i>CTX-M9 (23.1%), <i>bla</i>SHV (19.2%) and <i>bla</i>OXA-10 (15.4%). Genes associated with chloramphenicol resistance were <i>flo</i>R (65.4%) and <i>cml</i>A1 (46.2%). Tetracycline- and quinolone-resistance-associated genes <i>tet</i>A and <i>qnr</i>S were detected in (57.7%) and (50.0%) of isolates, respectively. The aminoglycoside resistance associated genes <i>aad</i>A1 (65.4%), <i>aad</i>A2 (53.8%), <i>aph</i>A (50.0%), <i>str</i>A (69.2%), and <i>str</i>B (65.4%), were detected among isolates. Macrolide resistance associated genes <i>mph</i> and <i>mrx</i> were also detected in (53.8%) and (34.6%). Moreover, colistin resistance associated gene <i>mcr</i>-9 was identified in one isolate (3.8%). The class 1 integron integrase <i>int</i>I1 (84.6%), transposase for the transposon <i>tnp</i>ISEcp1 (34.6%) and OqxB -integral membrane and component of RND-type multidrug efflux pump <i>oqx</i>B (7.7%) were identified among the isolates. The existing high incidence of ESBL/colistin-producing <i>E. coli</i> identified in healthy turkeys is a major concern that demands prompt control; otherwise, such strains and their resistance determinants could be transmitted to other bacteria and, eventually, to people via the food chain.https://www.mdpi.com/2079-6382/11/8/1075colistinESBLcarbapenemases<i>E. coli</i>Turkey (<i>Meleagris gallopavo</i>)microarray |
| spellingShingle | Amira A. Moawad Helmut Hotzel Hafez M. Hafez Hazem Ramadan Herbert Tomaso Sascha D. Braun Ralf Ehricht Celia Diezel Dominik Gary Ines Engelmann Islam M. Zakaria Reem M. Reda Samah Eid Momtaz A. Shahien Heinrich Neubauer Stefan Monecke Occurrence, Phenotypic and Molecular Characteristics of Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i> in Healthy Turkeys in Northern Egypt Antibiotics colistin ESBL carbapenemases <i>E. coli</i> Turkey (<i>Meleagris gallopavo</i>) microarray |
| title | Occurrence, Phenotypic and Molecular Characteristics of Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i> in Healthy Turkeys in Northern Egypt |
| title_full | Occurrence, Phenotypic and Molecular Characteristics of Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i> in Healthy Turkeys in Northern Egypt |
| title_fullStr | Occurrence, Phenotypic and Molecular Characteristics of Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i> in Healthy Turkeys in Northern Egypt |
| title_full_unstemmed | Occurrence, Phenotypic and Molecular Characteristics of Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i> in Healthy Turkeys in Northern Egypt |
| title_short | Occurrence, Phenotypic and Molecular Characteristics of Extended-Spectrum Beta-Lactamase-Producing <i>Escherichia coli</i> in Healthy Turkeys in Northern Egypt |
| title_sort | occurrence phenotypic and molecular characteristics of extended spectrum beta lactamase producing i escherichia coli i in healthy turkeys in northern egypt |
| topic | colistin ESBL carbapenemases <i>E. coli</i> Turkey (<i>Meleagris gallopavo</i>) microarray |
| url | https://www.mdpi.com/2079-6382/11/8/1075 |
| work_keys_str_mv | AT amiraamoawad occurrencephenotypicandmolecularcharacteristicsofextendedspectrumbetalactamaseproducingiescherichiacoliiinhealthyturkeysinnorthernegypt AT helmuthotzel occurrencephenotypicandmolecularcharacteristicsofextendedspectrumbetalactamaseproducingiescherichiacoliiinhealthyturkeysinnorthernegypt AT hafezmhafez occurrencephenotypicandmolecularcharacteristicsofextendedspectrumbetalactamaseproducingiescherichiacoliiinhealthyturkeysinnorthernegypt AT hazemramadan occurrencephenotypicandmolecularcharacteristicsofextendedspectrumbetalactamaseproducingiescherichiacoliiinhealthyturkeysinnorthernegypt AT herberttomaso occurrencephenotypicandmolecularcharacteristicsofextendedspectrumbetalactamaseproducingiescherichiacoliiinhealthyturkeysinnorthernegypt AT saschadbraun occurrencephenotypicandmolecularcharacteristicsofextendedspectrumbetalactamaseproducingiescherichiacoliiinhealthyturkeysinnorthernegypt AT ralfehricht occurrencephenotypicandmolecularcharacteristicsofextendedspectrumbetalactamaseproducingiescherichiacoliiinhealthyturkeysinnorthernegypt AT celiadiezel occurrencephenotypicandmolecularcharacteristicsofextendedspectrumbetalactamaseproducingiescherichiacoliiinhealthyturkeysinnorthernegypt AT dominikgary occurrencephenotypicandmolecularcharacteristicsofextendedspectrumbetalactamaseproducingiescherichiacoliiinhealthyturkeysinnorthernegypt AT inesengelmann occurrencephenotypicandmolecularcharacteristicsofextendedspectrumbetalactamaseproducingiescherichiacoliiinhealthyturkeysinnorthernegypt AT islammzakaria occurrencephenotypicandmolecularcharacteristicsofextendedspectrumbetalactamaseproducingiescherichiacoliiinhealthyturkeysinnorthernegypt AT reemmreda occurrencephenotypicandmolecularcharacteristicsofextendedspectrumbetalactamaseproducingiescherichiacoliiinhealthyturkeysinnorthernegypt AT samaheid occurrencephenotypicandmolecularcharacteristicsofextendedspectrumbetalactamaseproducingiescherichiacoliiinhealthyturkeysinnorthernegypt AT momtazashahien occurrencephenotypicandmolecularcharacteristicsofextendedspectrumbetalactamaseproducingiescherichiacoliiinhealthyturkeysinnorthernegypt AT heinrichneubauer occurrencephenotypicandmolecularcharacteristicsofextendedspectrumbetalactamaseproducingiescherichiacoliiinhealthyturkeysinnorthernegypt AT stefanmonecke occurrencephenotypicandmolecularcharacteristicsofextendedspectrumbetalactamaseproducingiescherichiacoliiinhealthyturkeysinnorthernegypt |