EVALUATION OF THE CONTENT OF CD4+CD25+CD127LOW REGULATORY T CELLS AND THEIR FUNCTIONAL STATUS ACCORDING TO THE EXPRESSION OF CTLA-4 AND CD39 MOLECULES IN PATIENTS WITH RHEUMATOID ARTHRITIS
Objective. To study the content of the suppressor population of regulatory T cells (Treg) according to the expression of CD4, CD25, CD127 molecules, as well as the expression of two functional molecules (CTLA-4 and CD39) in patients with rheumatoid arthritis (RA). Material and methods. 16 samples of...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
IMA PRESS LLC
2014-06-01
|
| Series: | Научно-практическая ревматология |
| Subjects: | |
| Online Access: | https://rsp.mediar-press.net/rsp/article/view/1920 |
| _version_ | 1797862806423339008 |
|---|---|
| author | G. A. Zhulai E. K. Oleinik O. Yu. Barysheva A. V. Churov V. M. Oleinik P. N. Kravchenko A. A. Kuchin |
| author_facet | G. A. Zhulai E. K. Oleinik O. Yu. Barysheva A. V. Churov V. M. Oleinik P. N. Kravchenko A. A. Kuchin |
| author_sort | G. A. Zhulai |
| collection | DOAJ |
| description | Objective. To study the content of the suppressor population of regulatory T cells (Treg) according to the expression of CD4, CD25, CD127 molecules, as well as the expression of two functional molecules (CTLA-4 and CD39) in patients with rheumatoid arthritis (RA). Material and methods. 16 samples of peripheral blood of RA patients and 10 blood samples of healthy donors were analyzed. All patients received disease-modifying anti-rheumatic drugs. The expression level of all studied molecules was assessed by flow cytofluorometry. Results. The content of T-helpers in peripheral blood of RA patients was 36.3 ± 7.1% of the total number of lympho- cytes and was lower than that in the control (43.8 ± 6.2%, p<0.05). The number of activated CD4+CD25+ T cells in these patients increased twofold (RA 23.7 ± 9.8%, control 11.1 ± 2.0% of the number of CD4+ T cells, p<0.05). The relative amount of CD4+CD25high (RA 2.7 ± 1.0%, control 1.5 ± 0.8%) and CD4+CD25highCD127low/- (RA 2.5 ± 1.0%, control 1.6 ± 0.9% of CD4+ T cells) Treg cells in RA patients was significantly higher than that in healthy individuals (p<0.05). The expression of the CTLA-4 negative regulatory molecule and CD39 ectonucleotidase by Treg cells of RA patients did not differ from the control. Conclusion. The study demonstrated that RA is characterized by an increased content of Treg cells of CD4+CD25high and CD4+CD25highCD127low phenotypes rather than CD4+CD25+CD127low, as well as by the control-like expression of CTLA-4 and CD39 functional molecules by Treg cells. |
| first_indexed | 2024-04-09T22:25:31Z |
| format | Article |
| id | doaj.art-bbfb9c1fc1d846fe8a826eb97a350b34 |
| institution | Directory Open Access Journal |
| issn | 1995-4484 1995-4492 |
| language | Russian |
| last_indexed | 2024-04-09T22:25:31Z |
| publishDate | 2014-06-01 |
| publisher | IMA PRESS LLC |
| record_format | Article |
| series | Научно-практическая ревматология |
| spelling | doaj.art-bbfb9c1fc1d846fe8a826eb97a350b342023-03-22T13:45:49ZrusIMA PRESS LLCНаучно-практическая ревматология1995-44841995-44922014-06-0152216917310.14412/1995-4484-2014-169-1731855EVALUATION OF THE CONTENT OF CD4+CD25+CD127LOW REGULATORY T CELLS AND THEIR FUNCTIONAL STATUS ACCORDING TO THE EXPRESSION OF CTLA-4 AND CD39 MOLECULES IN PATIENTS WITH RHEUMATOID ARTHRITISG. A. Zhulai0E. K. Oleinik1O. Yu. Barysheva2A. V. Churov3V. M. Oleinik4P. N. Kravchenko5A. A. Kuchin6Institute of Biology, Karelian Research Center of the Russian Academy of Sciences, Petrozavodsk, RussiaInstitute of Biology, Karelian Research Center of the Russian Academy of Sciences, Petrozavodsk, RussiaPetrozavodsk State University, Petrozavodsk, RussiaInstitute of Biology, Karelian Research Center of the Russian Academy of Sciences, Petrozavodsk, RussiaInstitute of Biology, Karelian Research Center of the Russian Academy of Sciences, Petrozavodsk, RussiaInstitute of Biology, Karelian Research Center of the Russian Academy of Sciences, Petrozavodsk, RussiaPetrozavodsk State University, Petrozavodsk, RussiaObjective. To study the content of the suppressor population of regulatory T cells (Treg) according to the expression of CD4, CD25, CD127 molecules, as well as the expression of two functional molecules (CTLA-4 and CD39) in patients with rheumatoid arthritis (RA). Material and methods. 16 samples of peripheral blood of RA patients and 10 blood samples of healthy donors were analyzed. All patients received disease-modifying anti-rheumatic drugs. The expression level of all studied molecules was assessed by flow cytofluorometry. Results. The content of T-helpers in peripheral blood of RA patients was 36.3 ± 7.1% of the total number of lympho- cytes and was lower than that in the control (43.8 ± 6.2%, p<0.05). The number of activated CD4+CD25+ T cells in these patients increased twofold (RA 23.7 ± 9.8%, control 11.1 ± 2.0% of the number of CD4+ T cells, p<0.05). The relative amount of CD4+CD25high (RA 2.7 ± 1.0%, control 1.5 ± 0.8%) and CD4+CD25highCD127low/- (RA 2.5 ± 1.0%, control 1.6 ± 0.9% of CD4+ T cells) Treg cells in RA patients was significantly higher than that in healthy individuals (p<0.05). The expression of the CTLA-4 negative regulatory molecule and CD39 ectonucleotidase by Treg cells of RA patients did not differ from the control. Conclusion. The study demonstrated that RA is characterized by an increased content of Treg cells of CD4+CD25high and CD4+CD25highCD127low phenotypes rather than CD4+CD25+CD127low, as well as by the control-like expression of CTLA-4 and CD39 functional molecules by Treg cells.https://rsp.mediar-press.net/rsp/article/view/1920rheumatoid arthritistreg cellsctla-4cd39. |
| spellingShingle | G. A. Zhulai E. K. Oleinik O. Yu. Barysheva A. V. Churov V. M. Oleinik P. N. Kravchenko A. A. Kuchin EVALUATION OF THE CONTENT OF CD4+CD25+CD127LOW REGULATORY T CELLS AND THEIR FUNCTIONAL STATUS ACCORDING TO THE EXPRESSION OF CTLA-4 AND CD39 MOLECULES IN PATIENTS WITH RHEUMATOID ARTHRITIS Научно-практическая ревматология rheumatoid arthritis treg cells ctla-4 cd39. |
| title | EVALUATION OF THE CONTENT OF CD4+CD25+CD127LOW REGULATORY T CELLS AND THEIR FUNCTIONAL STATUS ACCORDING TO THE EXPRESSION OF CTLA-4 AND CD39 MOLECULES IN PATIENTS WITH RHEUMATOID ARTHRITIS |
| title_full | EVALUATION OF THE CONTENT OF CD4+CD25+CD127LOW REGULATORY T CELLS AND THEIR FUNCTIONAL STATUS ACCORDING TO THE EXPRESSION OF CTLA-4 AND CD39 MOLECULES IN PATIENTS WITH RHEUMATOID ARTHRITIS |
| title_fullStr | EVALUATION OF THE CONTENT OF CD4+CD25+CD127LOW REGULATORY T CELLS AND THEIR FUNCTIONAL STATUS ACCORDING TO THE EXPRESSION OF CTLA-4 AND CD39 MOLECULES IN PATIENTS WITH RHEUMATOID ARTHRITIS |
| title_full_unstemmed | EVALUATION OF THE CONTENT OF CD4+CD25+CD127LOW REGULATORY T CELLS AND THEIR FUNCTIONAL STATUS ACCORDING TO THE EXPRESSION OF CTLA-4 AND CD39 MOLECULES IN PATIENTS WITH RHEUMATOID ARTHRITIS |
| title_short | EVALUATION OF THE CONTENT OF CD4+CD25+CD127LOW REGULATORY T CELLS AND THEIR FUNCTIONAL STATUS ACCORDING TO THE EXPRESSION OF CTLA-4 AND CD39 MOLECULES IN PATIENTS WITH RHEUMATOID ARTHRITIS |
| title_sort | evaluation of the content of cd4 cd25 cd127low regulatory t cells and their functional status according to the expression of ctla 4 and cd39 molecules in patients with rheumatoid arthritis |
| topic | rheumatoid arthritis treg cells ctla-4 cd39. |
| url | https://rsp.mediar-press.net/rsp/article/view/1920 |
| work_keys_str_mv | AT gazhulai evaluationofthecontentofcd4cd25cd127lowregulatorytcellsandtheirfunctionalstatusaccordingtotheexpressionofctla4andcd39moleculesinpatientswithrheumatoidarthritis AT ekoleinik evaluationofthecontentofcd4cd25cd127lowregulatorytcellsandtheirfunctionalstatusaccordingtotheexpressionofctla4andcd39moleculesinpatientswithrheumatoidarthritis AT oyubarysheva evaluationofthecontentofcd4cd25cd127lowregulatorytcellsandtheirfunctionalstatusaccordingtotheexpressionofctla4andcd39moleculesinpatientswithrheumatoidarthritis AT avchurov evaluationofthecontentofcd4cd25cd127lowregulatorytcellsandtheirfunctionalstatusaccordingtotheexpressionofctla4andcd39moleculesinpatientswithrheumatoidarthritis AT vmoleinik evaluationofthecontentofcd4cd25cd127lowregulatorytcellsandtheirfunctionalstatusaccordingtotheexpressionofctla4andcd39moleculesinpatientswithrheumatoidarthritis AT pnkravchenko evaluationofthecontentofcd4cd25cd127lowregulatorytcellsandtheirfunctionalstatusaccordingtotheexpressionofctla4andcd39moleculesinpatientswithrheumatoidarthritis AT aakuchin evaluationofthecontentofcd4cd25cd127lowregulatorytcellsandtheirfunctionalstatusaccordingtotheexpressionofctla4andcd39moleculesinpatientswithrheumatoidarthritis |