Antennal transcriptome analysis of the chemosensory gene families in Carposina sasakii (Lepidoptera: Carposinidae)
Abstract Background The peach fruit moth, Carposina sasakii Matsumura (Lepidoptera: Carposinidae), poses a serious threat to a variety of fruits and causes significant economic loss owing to difficulties in its prevention and control. The olfactory sense is generally acknowledged to be a novel targe...
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| Format: | Article |
| Language: | English |
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BMC
2018-07-01
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| Series: | BMC Genomics |
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| Online Access: | http://link.springer.com/article/10.1186/s12864-018-4900-x |
| _version_ | 1818543840230899712 |
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| author | Zhiqiang Tian Lina Sun Yanyan Li Linfa Quan Huaijiang Zhang Wentao Yan Qiang Yue Guisheng Qiu |
| author_facet | Zhiqiang Tian Lina Sun Yanyan Li Linfa Quan Huaijiang Zhang Wentao Yan Qiang Yue Guisheng Qiu |
| author_sort | Zhiqiang Tian |
| collection | DOAJ |
| description | Abstract Background The peach fruit moth, Carposina sasakii Matsumura (Lepidoptera: Carposinidae), poses a serious threat to a variety of fruits and causes significant economic loss owing to difficulties in its prevention and control. The olfactory sense is generally acknowledged to be a novel target for pest control. However, a systematic study of the olfactory genes expressed in C. sasakii has not been reported yet. Here, we reported the antennal transcriptome of C. sasakii using high-throughput sequencing and annotated the main chemosensory multi-gene families. Results In the chemosensory gene families, 29 odorant-binding proteins, 13 chemosensory proteins, 1 sensory neuron membrane protein, 52 odorant receptors, 8 ionotropic receptors and 11 gustatory receptors were annotated in the C. sasakii antennal transcriptome. The number of olfactory genes obtained in our transcriptome was consistent with that identified in other lepidopteran insects, confirming that we basically accomplished the annotation of the chemosensory genes of C. sasakii in the adult antennal transcriptome. All sequences were annotated and analyzed by BLAST (basic local alignment search tool), and some chemosensory genes with specific functions were named according to the BLAST results and phylogenetic trees. Based on the expression profile in the transcriptome and phylogenetic analysis, differentially expressed genes (DEGs) were analyzed in both male and female adults. Finally, fluorescence quantitative real-time PCR was used to identify the male-specific or female-specific chemosensory genes that were putatively related to odor detection and recognition. Moreover, expression levels of OR33 and PBP2 were significantly higher in males than in females, indicating that these genes may interact with sex pheromones. We found some conserved antennal IRs and GRs involved in detecting sugar compounds (GR2, GR5, GR6, GR8) and carbon dioxide (GR1), which were also identified based on phylogenetic analysis. Conclusions There are 114 putative chemosensory proteins expressed in C. sasakii identified in this study. The identification of these proteins will make the molecular mechanism of odor recognition accessible. |
| first_indexed | 2024-12-11T22:40:42Z |
| format | Article |
| id | doaj.art-bbfbb7c889ad40f7aa6606a23c5f0841 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-11T22:40:42Z |
| publishDate | 2018-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-bbfbb7c889ad40f7aa6606a23c5f08412022-12-22T00:47:48ZengBMCBMC Genomics1471-21642018-07-0119111610.1186/s12864-018-4900-xAntennal transcriptome analysis of the chemosensory gene families in Carposina sasakii (Lepidoptera: Carposinidae)Zhiqiang Tian0Lina Sun1Yanyan Li2Linfa Quan3Huaijiang Zhang4Wentao Yan5Qiang Yue6Guisheng Qiu7Research Institute of Pomology, Chinese Academy of Agricultural SciencesResearch Institute of Pomology, Chinese Academy of Agricultural SciencesResearch Institute of Pomology, Chinese Academy of Agricultural SciencesResearch Institute of Pomology, Chinese Academy of Agricultural SciencesResearch Institute of Pomology, Chinese Academy of Agricultural SciencesResearch Institute of Pomology, Chinese Academy of Agricultural SciencesResearch Institute of Pomology, Chinese Academy of Agricultural SciencesResearch Institute of Pomology, Chinese Academy of Agricultural SciencesAbstract Background The peach fruit moth, Carposina sasakii Matsumura (Lepidoptera: Carposinidae), poses a serious threat to a variety of fruits and causes significant economic loss owing to difficulties in its prevention and control. The olfactory sense is generally acknowledged to be a novel target for pest control. However, a systematic study of the olfactory genes expressed in C. sasakii has not been reported yet. Here, we reported the antennal transcriptome of C. sasakii using high-throughput sequencing and annotated the main chemosensory multi-gene families. Results In the chemosensory gene families, 29 odorant-binding proteins, 13 chemosensory proteins, 1 sensory neuron membrane protein, 52 odorant receptors, 8 ionotropic receptors and 11 gustatory receptors were annotated in the C. sasakii antennal transcriptome. The number of olfactory genes obtained in our transcriptome was consistent with that identified in other lepidopteran insects, confirming that we basically accomplished the annotation of the chemosensory genes of C. sasakii in the adult antennal transcriptome. All sequences were annotated and analyzed by BLAST (basic local alignment search tool), and some chemosensory genes with specific functions were named according to the BLAST results and phylogenetic trees. Based on the expression profile in the transcriptome and phylogenetic analysis, differentially expressed genes (DEGs) were analyzed in both male and female adults. Finally, fluorescence quantitative real-time PCR was used to identify the male-specific or female-specific chemosensory genes that were putatively related to odor detection and recognition. Moreover, expression levels of OR33 and PBP2 were significantly higher in males than in females, indicating that these genes may interact with sex pheromones. We found some conserved antennal IRs and GRs involved in detecting sugar compounds (GR2, GR5, GR6, GR8) and carbon dioxide (GR1), which were also identified based on phylogenetic analysis. Conclusions There are 114 putative chemosensory proteins expressed in C. sasakii identified in this study. The identification of these proteins will make the molecular mechanism of odor recognition accessible.http://link.springer.com/article/10.1186/s12864-018-4900-xPeach fruit mothAntennal transcriptomeOlfactory geneExpression profile |
| spellingShingle | Zhiqiang Tian Lina Sun Yanyan Li Linfa Quan Huaijiang Zhang Wentao Yan Qiang Yue Guisheng Qiu Antennal transcriptome analysis of the chemosensory gene families in Carposina sasakii (Lepidoptera: Carposinidae) BMC Genomics Peach fruit moth Antennal transcriptome Olfactory gene Expression profile |
| title | Antennal transcriptome analysis of the chemosensory gene families in Carposina sasakii (Lepidoptera: Carposinidae) |
| title_full | Antennal transcriptome analysis of the chemosensory gene families in Carposina sasakii (Lepidoptera: Carposinidae) |
| title_fullStr | Antennal transcriptome analysis of the chemosensory gene families in Carposina sasakii (Lepidoptera: Carposinidae) |
| title_full_unstemmed | Antennal transcriptome analysis of the chemosensory gene families in Carposina sasakii (Lepidoptera: Carposinidae) |
| title_short | Antennal transcriptome analysis of the chemosensory gene families in Carposina sasakii (Lepidoptera: Carposinidae) |
| title_sort | antennal transcriptome analysis of the chemosensory gene families in carposina sasakii lepidoptera carposinidae |
| topic | Peach fruit moth Antennal transcriptome Olfactory gene Expression profile |
| url | http://link.springer.com/article/10.1186/s12864-018-4900-x |
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