In-silico characterization of phytochemicals identified from Vitex negundo (L) extract as potential therapy for Wnt-signaling proteins
Abstract Background Colorectal cancer is the third most diagnosed disease in the world population and current chemotherapy has been used for targeting the cell proliferation and metastasizing ability of tumor cells. Potent chemotherapeutic drugs for colorectal cancer are capecitabine, fluorouracil,...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2022-01-01
|
| Series: | Egyptian Journal of Medical Human Genetics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43042-022-00219-7 |
| _version_ | 1798026261058027520 |
|---|---|
| author | Kuruvalli Gouthami Vadamalai Veeraraghavan Prashantha Nagaraja |
| author_facet | Kuruvalli Gouthami Vadamalai Veeraraghavan Prashantha Nagaraja |
| author_sort | Kuruvalli Gouthami |
| collection | DOAJ |
| description | Abstract Background Colorectal cancer is the third most diagnosed disease in the world population and current chemotherapy has been used for targeting the cell proliferation and metastasizing ability of tumor cells. Potent chemotherapeutic drugs for colorectal cancer are capecitabine, fluorouracil, irinotecan, etc. which have toxic effects in normal tissues and adverse effects in multiple organs leading to major obstacles in clinical use. The aim of the study is the use of plant-derived compounds that improve the effectiveness of chemotherapeutics with lower and alleviate toxic side effects and reduce the risk of tumor progression. Results The current study is performed using Vitex negundo leaf which has been demonstrated to have positive effects against colorectal cancer. The use of computational approaches will help improve the identification and screening of lead molecules using AutoDock 4.2 and AutoDock Vina. Using computational approaches will help to improve lead identification and screening. Herein, we have retrieved six phytochemicals from published literature and investigated their inhibitory effect with Wnt-associated signaling proteins. Authentication of phytocompounds and Wnt-associated signaling proteins was done using AutoDock.4.2. Conclusions The results are screened based on the number of hydrogen bonds, binding energy, and interacting amino acids. The Isoorientin, luteolin, and Chrysophanol get the highest binding energy with target receptors. The binding energy is calculated with all target receptors from the range of − 6.0 to − 8.9 kcal/mol. The In-silico drug likeliness properties are predicted to be the best interacting compounds based on Lipinski Rule of 5 and ADMET analysis. Hence, we propose that Isoorientin, luteolin, and Chrysophanol are the potential inhibitors of Wnt signaling inhibitors, and preclinical studies are needed to confirm the promising therapeutic ability of colorectal cancer. |
| first_indexed | 2024-04-11T18:32:25Z |
| format | Article |
| id | doaj.art-bbfbd1e381bd4f6badb71e332917b325 |
| institution | Directory Open Access Journal |
| issn | 2090-2441 |
| language | English |
| last_indexed | 2024-04-11T18:32:25Z |
| publishDate | 2022-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Egyptian Journal of Medical Human Genetics |
| spelling | doaj.art-bbfbd1e381bd4f6badb71e332917b3252022-12-22T04:09:25ZengSpringerOpenEgyptian Journal of Medical Human Genetics2090-24412022-01-0123111510.1186/s43042-022-00219-7In-silico characterization of phytochemicals identified from Vitex negundo (L) extract as potential therapy for Wnt-signaling proteinsKuruvalli Gouthami0Vadamalai Veeraraghavan1Prashantha Nagaraja2Department of Biochemistry, School of Applied Sciences, REVA UniversityDepartment of Biochemistry, School of Applied Sciences, REVA UniversityDepartment of Biotechnology, School of Applied Sciences, REVA UniversityAbstract Background Colorectal cancer is the third most diagnosed disease in the world population and current chemotherapy has been used for targeting the cell proliferation and metastasizing ability of tumor cells. Potent chemotherapeutic drugs for colorectal cancer are capecitabine, fluorouracil, irinotecan, etc. which have toxic effects in normal tissues and adverse effects in multiple organs leading to major obstacles in clinical use. The aim of the study is the use of plant-derived compounds that improve the effectiveness of chemotherapeutics with lower and alleviate toxic side effects and reduce the risk of tumor progression. Results The current study is performed using Vitex negundo leaf which has been demonstrated to have positive effects against colorectal cancer. The use of computational approaches will help improve the identification and screening of lead molecules using AutoDock 4.2 and AutoDock Vina. Using computational approaches will help to improve lead identification and screening. Herein, we have retrieved six phytochemicals from published literature and investigated their inhibitory effect with Wnt-associated signaling proteins. Authentication of phytocompounds and Wnt-associated signaling proteins was done using AutoDock.4.2. Conclusions The results are screened based on the number of hydrogen bonds, binding energy, and interacting amino acids. The Isoorientin, luteolin, and Chrysophanol get the highest binding energy with target receptors. The binding energy is calculated with all target receptors from the range of − 6.0 to − 8.9 kcal/mol. The In-silico drug likeliness properties are predicted to be the best interacting compounds based on Lipinski Rule of 5 and ADMET analysis. Hence, we propose that Isoorientin, luteolin, and Chrysophanol are the potential inhibitors of Wnt signaling inhibitors, and preclinical studies are needed to confirm the promising therapeutic ability of colorectal cancer.https://doi.org/10.1186/s43042-022-00219-7WNTColorectal cancerPharmacophoreDockingDrug likeliness |
| spellingShingle | Kuruvalli Gouthami Vadamalai Veeraraghavan Prashantha Nagaraja In-silico characterization of phytochemicals identified from Vitex negundo (L) extract as potential therapy for Wnt-signaling proteins Egyptian Journal of Medical Human Genetics WNT Colorectal cancer Pharmacophore Docking Drug likeliness |
| title | In-silico characterization of phytochemicals identified from Vitex negundo (L) extract as potential therapy for Wnt-signaling proteins |
| title_full | In-silico characterization of phytochemicals identified from Vitex negundo (L) extract as potential therapy for Wnt-signaling proteins |
| title_fullStr | In-silico characterization of phytochemicals identified from Vitex negundo (L) extract as potential therapy for Wnt-signaling proteins |
| title_full_unstemmed | In-silico characterization of phytochemicals identified from Vitex negundo (L) extract as potential therapy for Wnt-signaling proteins |
| title_short | In-silico characterization of phytochemicals identified from Vitex negundo (L) extract as potential therapy for Wnt-signaling proteins |
| title_sort | in silico characterization of phytochemicals identified from vitex negundo l extract as potential therapy for wnt signaling proteins |
| topic | WNT Colorectal cancer Pharmacophore Docking Drug likeliness |
| url | https://doi.org/10.1186/s43042-022-00219-7 |
| work_keys_str_mv | AT kuruvalligouthami insilicocharacterizationofphytochemicalsidentifiedfromvitexnegundolextractaspotentialtherapyforwntsignalingproteins AT vadamalaiveeraraghavan insilicocharacterizationofphytochemicalsidentifiedfromvitexnegundolextractaspotentialtherapyforwntsignalingproteins AT prashanthanagaraja insilicocharacterizationofphytochemicalsidentifiedfromvitexnegundolextractaspotentialtherapyforwntsignalingproteins |