In vitro cost-effective methods to detect carbapenemases in Enterobacteriaceae
The rise in carbapenemases-producing organisms has challenged the scientific community. Infections caused by these bacteria have limited treatment options. There are various types such as Klebsiella pneumoniae carbapenemase (Ambler class A), metallo-beta-lactamases of VIM-type, IMP-type, NDM-type (A...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Thieme Medical and Scientific Publishers Pvt. Ltd.
2018-01-01
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| Series: | Journal of Laboratory Physicians |
| Subjects: | |
| Online Access: | http://www.thieme-connect.de/DOI/DOI?10.4103/JLP.JLP_25_17 |
| _version_ | 1819049583404122112 |
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| author | Varsha Gupta Ranu Soni Neha Jain Jagdish Chander |
| author_facet | Varsha Gupta Ranu Soni Neha Jain Jagdish Chander |
| author_sort | Varsha Gupta |
| collection | DOAJ |
| description | The rise in carbapenemases-producing organisms has challenged the scientific community. Infections caused by these bacteria have limited treatment options. There are various types such as Klebsiella pneumoniae carbapenemase (Ambler class A), metallo-beta-lactamases of VIM-type, IMP-type, NDM-type (Ambler class B), and OXA-48-types (Ambler class D). An efficient strategy for detection of carbapenemase producers is important to determine the appropriate therapeutic modalities. In this study, four methods - Carba NP test, modified Carba NP (MCNP) test, carbapenem inactivation method (CIM) test, and Rapidec Carba NP kit test were evaluated. We evaluated an in-house MCNP test to detect carbapenemase production using a single protocol which gave reliable results. Furthermore, CIM using routine antibiotic discs gives good results. Both these tests were found to be cost-effective. |
| first_indexed | 2024-12-21T11:34:27Z |
| format | Article |
| id | doaj.art-bbff02b855f0475ab796f2304fae5c69 |
| institution | Directory Open Access Journal |
| issn | 0974-2727 0974-7826 |
| language | English |
| last_indexed | 2024-12-21T11:34:27Z |
| publishDate | 2018-01-01 |
| publisher | Thieme Medical and Scientific Publishers Pvt. Ltd. |
| record_format | Article |
| series | Journal of Laboratory Physicians |
| spelling | doaj.art-bbff02b855f0475ab796f2304fae5c692022-12-21T19:05:28ZengThieme Medical and Scientific Publishers Pvt. Ltd.Journal of Laboratory Physicians0974-27270974-78262018-01-01100110110510.4103/JLP.JLP_25_17In vitro cost-effective methods to detect carbapenemases in EnterobacteriaceaeVarsha Gupta0Ranu Soni1Neha Jain2Jagdish Chander3Department of Microbiology, Government Medical College Hospital, Chandigarh, IndiaDepartment of Microbiology, Government Medical College Hospital, Chandigarh, IndiaDepartment of Microbiology, Government Medical College Hospital, Chandigarh, IndiaDepartment of Microbiology, Government Medical College Hospital, Chandigarh, IndiaThe rise in carbapenemases-producing organisms has challenged the scientific community. Infections caused by these bacteria have limited treatment options. There are various types such as Klebsiella pneumoniae carbapenemase (Ambler class A), metallo-beta-lactamases of VIM-type, IMP-type, NDM-type (Ambler class B), and OXA-48-types (Ambler class D). An efficient strategy for detection of carbapenemase producers is important to determine the appropriate therapeutic modalities. In this study, four methods - Carba NP test, modified Carba NP (MCNP) test, carbapenem inactivation method (CIM) test, and Rapidec Carba NP kit test were evaluated. We evaluated an in-house MCNP test to detect carbapenemase production using a single protocol which gave reliable results. Furthermore, CIM using routine antibiotic discs gives good results. Both these tests were found to be cost-effective.http://www.thieme-connect.de/DOI/DOI?10.4103/JLP.JLP_25_17carbapenemases enterobacteriaceae imipenem |
| spellingShingle | Varsha Gupta Ranu Soni Neha Jain Jagdish Chander In vitro cost-effective methods to detect carbapenemases in Enterobacteriaceae Journal of Laboratory Physicians carbapenemases enterobacteriaceae imipenem |
| title | In vitro cost-effective methods to detect carbapenemases in Enterobacteriaceae |
| title_full | In vitro cost-effective methods to detect carbapenemases in Enterobacteriaceae |
| title_fullStr | In vitro cost-effective methods to detect carbapenemases in Enterobacteriaceae |
| title_full_unstemmed | In vitro cost-effective methods to detect carbapenemases in Enterobacteriaceae |
| title_short | In vitro cost-effective methods to detect carbapenemases in Enterobacteriaceae |
| title_sort | in vitro cost effective methods to detect carbapenemases in enterobacteriaceae |
| topic | carbapenemases enterobacteriaceae imipenem |
| url | http://www.thieme-connect.de/DOI/DOI?10.4103/JLP.JLP_25_17 |
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