Precision and Accuracy of Field Versus Laboratory Bioassay Insecticide Efficacy for the Control of Immature <i>Bemisia tabaci</i>
Ecotoxicological studies often result in reports on the limitation and sometime failures of biological assay data to predict field response to similar treatments. Nevertheless, it is widely accepted that controlled bioassays can better quantify the specific mortality response of a target pest specie...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/14/7/645 |
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| author | Paulo S. G. Cremonez Jermaine D. Perier Mirela M. Nagaoka Alvin M. Simmons David G. Riley |
| author_facet | Paulo S. G. Cremonez Jermaine D. Perier Mirela M. Nagaoka Alvin M. Simmons David G. Riley |
| author_sort | Paulo S. G. Cremonez |
| collection | DOAJ |
| description | Ecotoxicological studies often result in reports on the limitation and sometime failures of biological assay data to predict field response to similar treatments. Nevertheless, it is widely accepted that controlled bioassays can better quantify the specific mortality response of a target pest species to a specific toxin. To quantify the relationship between whitefly bioassay and field response data, we evaluated a controlled laboratory bioassay and a concurrent cucurbit field trial method to assess insecticide efficacy for controlling the sweetpotato whitefly, <i>Bemisia tabaci</i> (Gennadius) (Hemiptera: Aleyrodidae). This was based on oviposition and nymphal development. We specifically tested the assumptions that a maximum dose bioassay would more precisely measure insecticide efficacy as compared with a comparable field spray test evaluation, and the response would be equal between the bioassay and the field as a measure of control accuracy for both adult oviposition and development of nymphal stages. To make a direct comparison, we tested the same whitefly population subsamples from 352 plots in eight cucurbit field experiments in Georgia, USA, in 2021 and 2022. The bioassays provide significantly precision for estimating proportional whitefly response. As expected, treatment-specific nonequivalence in immature whitefly counts between the bioassay and field, i.e., a lack of accuracy, only occurred with insecticides that were not highly toxic to all growth stages of whiteflies. |
| first_indexed | 2024-03-11T00:59:01Z |
| format | Article |
| id | doaj.art-bbff1ca1b0ca46efbeeb4848ed15c3f5 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-11T00:59:01Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-bbff1ca1b0ca46efbeeb4848ed15c3f52023-11-18T19:49:28ZengMDPI AGInsects2075-44502023-07-0114764510.3390/insects14070645Precision and Accuracy of Field Versus Laboratory Bioassay Insecticide Efficacy for the Control of Immature <i>Bemisia tabaci</i>Paulo S. G. Cremonez0Jermaine D. Perier1Mirela M. Nagaoka2Alvin M. Simmons3David G. Riley4Department of Entomology, University of Georgia Tifton Campus, Tifton, GA 31793, USADepartment of Entomology, University of Georgia Tifton Campus, Tifton, GA 31793, USADepartment of Entomology, University of Georgia Tifton Campus, Tifton, GA 31793, USAU.S. Vegetable Laboratory, Agricultural Research Service, USDA, 2700 Savannah Highway, Charleston, SC 29414, USADepartment of Entomology, University of Georgia Tifton Campus, Tifton, GA 31793, USAEcotoxicological studies often result in reports on the limitation and sometime failures of biological assay data to predict field response to similar treatments. Nevertheless, it is widely accepted that controlled bioassays can better quantify the specific mortality response of a target pest species to a specific toxin. To quantify the relationship between whitefly bioassay and field response data, we evaluated a controlled laboratory bioassay and a concurrent cucurbit field trial method to assess insecticide efficacy for controlling the sweetpotato whitefly, <i>Bemisia tabaci</i> (Gennadius) (Hemiptera: Aleyrodidae). This was based on oviposition and nymphal development. We specifically tested the assumptions that a maximum dose bioassay would more precisely measure insecticide efficacy as compared with a comparable field spray test evaluation, and the response would be equal between the bioassay and the field as a measure of control accuracy for both adult oviposition and development of nymphal stages. To make a direct comparison, we tested the same whitefly population subsamples from 352 plots in eight cucurbit field experiments in Georgia, USA, in 2021 and 2022. The bioassays provide significantly precision for estimating proportional whitefly response. As expected, treatment-specific nonequivalence in immature whitefly counts between the bioassay and field, i.e., a lack of accuracy, only occurred with insecticides that were not highly toxic to all growth stages of whiteflies.https://www.mdpi.com/2075-4450/14/7/645insecticide resistance management (IRM)maximum-dose bioassaysweetpotato whitefly<i>Bemisia tabaci</i>insecticide response |
| spellingShingle | Paulo S. G. Cremonez Jermaine D. Perier Mirela M. Nagaoka Alvin M. Simmons David G. Riley Precision and Accuracy of Field Versus Laboratory Bioassay Insecticide Efficacy for the Control of Immature <i>Bemisia tabaci</i> Insects insecticide resistance management (IRM) maximum-dose bioassay sweetpotato whitefly <i>Bemisia tabaci</i> insecticide response |
| title | Precision and Accuracy of Field Versus Laboratory Bioassay Insecticide Efficacy for the Control of Immature <i>Bemisia tabaci</i> |
| title_full | Precision and Accuracy of Field Versus Laboratory Bioassay Insecticide Efficacy for the Control of Immature <i>Bemisia tabaci</i> |
| title_fullStr | Precision and Accuracy of Field Versus Laboratory Bioassay Insecticide Efficacy for the Control of Immature <i>Bemisia tabaci</i> |
| title_full_unstemmed | Precision and Accuracy of Field Versus Laboratory Bioassay Insecticide Efficacy for the Control of Immature <i>Bemisia tabaci</i> |
| title_short | Precision and Accuracy of Field Versus Laboratory Bioassay Insecticide Efficacy for the Control of Immature <i>Bemisia tabaci</i> |
| title_sort | precision and accuracy of field versus laboratory bioassay insecticide efficacy for the control of immature i bemisia tabaci i |
| topic | insecticide resistance management (IRM) maximum-dose bioassay sweetpotato whitefly <i>Bemisia tabaci</i> insecticide response |
| url | https://www.mdpi.com/2075-4450/14/7/645 |
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