Metabolomic Profiling and Antioxidant Activity of Fruits Representing Diverse Apple and Pear Cultivars
The false fruits of apple (<i>Malus domestica</i>) and pear (<i>Pyrus communis</i>) are consumed all over the world, contributing to the dietary intake of health-promoting antioxidant phytochemicals. For example, polyphenols confer many beneficial effects (according to their...
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MDPI AG
2021-04-01
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| Online Access: | https://www.mdpi.com/2079-7737/10/5/380 |
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| author | Mauro Commisso Martino Bianconi Stefania Poletti Stefano Negri Francesca Munari Stefania Ceoldo Flavia Guzzo |
| author_facet | Mauro Commisso Martino Bianconi Stefania Poletti Stefano Negri Francesca Munari Stefania Ceoldo Flavia Guzzo |
| author_sort | Mauro Commisso |
| collection | DOAJ |
| description | The false fruits of apple (<i>Malus domestica</i>) and pear (<i>Pyrus communis</i>) are consumed all over the world, contributing to the dietary intake of health-promoting antioxidant phytochemicals. For example, polyphenols confer many beneficial effects (according to their chemical structure, bioavailability, and absorption efficiency in the gut) and the consumption of polyphenol-rich apple and pear fruits may therefore reduce the risk of some diseases. However, the content of such molecules is highly dependent on the specific fruit cultivar. To examine this metabolic diversity in detail, we used metabolomic analysis (NMR and HPLC-DAD/MS) to profile the metabolome of six apple and five pear cultivars. We also determined the antioxidant capacity of the extracts (FRAP assay) and correlated this with the metabolomic composition and abundance of specific metabolites. We observed the cultivar-specific accumulation of sugars, amino acids, malic acid, and various polyphenols, which was also related to the growing season for some cultivars. We found that the ancient Italian apple Pom Prussian was enriched for chlorogenic acid as well as more characteristic polyphenols (phloretin derivatives), the pear cultivar Abate Fetel was low in sucrose, and both cultivars displayed high in vitro antioxidant activity. These cultivars may, therefore, be particularly attractive to health-conscious consumers. |
| first_indexed | 2024-03-10T11:53:22Z |
| format | Article |
| id | doaj.art-bc00c3dc6e994944a98e7935bed1a471 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T11:53:22Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-bc00c3dc6e994944a98e7935bed1a4712023-11-21T17:32:41ZengMDPI AGBiology2079-77372021-04-0110538010.3390/biology10050380Metabolomic Profiling and Antioxidant Activity of Fruits Representing Diverse Apple and Pear CultivarsMauro Commisso0Martino Bianconi1Stefania Poletti2Stefano Negri3Francesca Munari4Stefania Ceoldo5Flavia Guzzo6Biotechnology Department, University of Verona, Strada Le Grazie, 15, 37134 Verona, ItalyDemethra Biotech Srl, Via dell’Innovazione, 1, Camisano Vicentino, 36043 Vicenza, ItalyBiotechnology Department, University of Verona, Strada Le Grazie, 15, 37134 Verona, ItalyBiotechnology Department, University of Verona, Strada Le Grazie, 15, 37134 Verona, ItalyBiotechnology Department, University of Verona, Strada Le Grazie, 15, 37134 Verona, ItalyBiotechnology Department, University of Verona, Strada Le Grazie, 15, 37134 Verona, ItalyBiotechnology Department, University of Verona, Strada Le Grazie, 15, 37134 Verona, ItalyThe false fruits of apple (<i>Malus domestica</i>) and pear (<i>Pyrus communis</i>) are consumed all over the world, contributing to the dietary intake of health-promoting antioxidant phytochemicals. For example, polyphenols confer many beneficial effects (according to their chemical structure, bioavailability, and absorption efficiency in the gut) and the consumption of polyphenol-rich apple and pear fruits may therefore reduce the risk of some diseases. However, the content of such molecules is highly dependent on the specific fruit cultivar. To examine this metabolic diversity in detail, we used metabolomic analysis (NMR and HPLC-DAD/MS) to profile the metabolome of six apple and five pear cultivars. We also determined the antioxidant capacity of the extracts (FRAP assay) and correlated this with the metabolomic composition and abundance of specific metabolites. We observed the cultivar-specific accumulation of sugars, amino acids, malic acid, and various polyphenols, which was also related to the growing season for some cultivars. We found that the ancient Italian apple Pom Prussian was enriched for chlorogenic acid as well as more characteristic polyphenols (phloretin derivatives), the pear cultivar Abate Fetel was low in sucrose, and both cultivars displayed high in vitro antioxidant activity. These cultivars may, therefore, be particularly attractive to health-conscious consumers.https://www.mdpi.com/2079-7737/10/5/380applepearfruit polyphenolsantioxidantsuntargeted metabolomics |
| spellingShingle | Mauro Commisso Martino Bianconi Stefania Poletti Stefano Negri Francesca Munari Stefania Ceoldo Flavia Guzzo Metabolomic Profiling and Antioxidant Activity of Fruits Representing Diverse Apple and Pear Cultivars Biology apple pear fruit polyphenols antioxidants untargeted metabolomics |
| title | Metabolomic Profiling and Antioxidant Activity of Fruits Representing Diverse Apple and Pear Cultivars |
| title_full | Metabolomic Profiling and Antioxidant Activity of Fruits Representing Diverse Apple and Pear Cultivars |
| title_fullStr | Metabolomic Profiling and Antioxidant Activity of Fruits Representing Diverse Apple and Pear Cultivars |
| title_full_unstemmed | Metabolomic Profiling and Antioxidant Activity of Fruits Representing Diverse Apple and Pear Cultivars |
| title_short | Metabolomic Profiling and Antioxidant Activity of Fruits Representing Diverse Apple and Pear Cultivars |
| title_sort | metabolomic profiling and antioxidant activity of fruits representing diverse apple and pear cultivars |
| topic | apple pear fruit polyphenols antioxidants untargeted metabolomics |
| url | https://www.mdpi.com/2079-7737/10/5/380 |
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