Diverse Regulation of Temperature Sensation by Trimeric G-Protein Signaling in Caenorhabditis elegans.
Temperature sensation by the nervous system is essential for life and proliferation of animals. The molecular-physiological mechanisms underlying temperature signaling have not been fully elucidated. We show here that diverse regulatory machinery underlies temperature sensation through trimeric G-pr...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5082853?pdf=render |
| _version_ | 1819118631346241536 |
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| author | Tomoyo Ujisawa Akane Ohta Misato Uda-Yagi Atsushi Kuhara |
| author_facet | Tomoyo Ujisawa Akane Ohta Misato Uda-Yagi Atsushi Kuhara |
| author_sort | Tomoyo Ujisawa |
| collection | DOAJ |
| description | Temperature sensation by the nervous system is essential for life and proliferation of animals. The molecular-physiological mechanisms underlying temperature signaling have not been fully elucidated. We show here that diverse regulatory machinery underlies temperature sensation through trimeric G-protein signaling in the nematode Caenorhabditis elegans. Molecular-genetic studies demonstrated that cold tolerance is regulated by additive functions of three Gα proteins in a temperature-sensing neuron, ASJ, which is also known to be a light-sensing neuron. Optical recording of calcium concentration in ASJ upon temperature-changes demonstrated that three Gα proteins act in different aspects of temperature signaling. Calcium concentration changes in ASJ upon temperature change were unexpectedly decreased in a mutant defective in phosphodiesterase, which is well known as a negative regulator of calcium increase. Together, these data demonstrate commonalities and differences in the molecular components concerned with light and temperature signaling in a single sensory neuron. |
| first_indexed | 2024-12-22T05:51:57Z |
| format | Article |
| id | doaj.art-bc02c093913b4ab9a6fb8dcc7968f4a7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T05:51:57Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-bc02c093913b4ab9a6fb8dcc7968f4a72022-12-21T18:36:51ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-011110e016551810.1371/journal.pone.0165518Diverse Regulation of Temperature Sensation by Trimeric G-Protein Signaling in Caenorhabditis elegans.Tomoyo UjisawaAkane OhtaMisato Uda-YagiAtsushi KuharaTemperature sensation by the nervous system is essential for life and proliferation of animals. The molecular-physiological mechanisms underlying temperature signaling have not been fully elucidated. We show here that diverse regulatory machinery underlies temperature sensation through trimeric G-protein signaling in the nematode Caenorhabditis elegans. Molecular-genetic studies demonstrated that cold tolerance is regulated by additive functions of three Gα proteins in a temperature-sensing neuron, ASJ, which is also known to be a light-sensing neuron. Optical recording of calcium concentration in ASJ upon temperature-changes demonstrated that three Gα proteins act in different aspects of temperature signaling. Calcium concentration changes in ASJ upon temperature change were unexpectedly decreased in a mutant defective in phosphodiesterase, which is well known as a negative regulator of calcium increase. Together, these data demonstrate commonalities and differences in the molecular components concerned with light and temperature signaling in a single sensory neuron.http://europepmc.org/articles/PMC5082853?pdf=render |
| spellingShingle | Tomoyo Ujisawa Akane Ohta Misato Uda-Yagi Atsushi Kuhara Diverse Regulation of Temperature Sensation by Trimeric G-Protein Signaling in Caenorhabditis elegans. PLoS ONE |
| title | Diverse Regulation of Temperature Sensation by Trimeric G-Protein Signaling in Caenorhabditis elegans. |
| title_full | Diverse Regulation of Temperature Sensation by Trimeric G-Protein Signaling in Caenorhabditis elegans. |
| title_fullStr | Diverse Regulation of Temperature Sensation by Trimeric G-Protein Signaling in Caenorhabditis elegans. |
| title_full_unstemmed | Diverse Regulation of Temperature Sensation by Trimeric G-Protein Signaling in Caenorhabditis elegans. |
| title_short | Diverse Regulation of Temperature Sensation by Trimeric G-Protein Signaling in Caenorhabditis elegans. |
| title_sort | diverse regulation of temperature sensation by trimeric g protein signaling in caenorhabditis elegans |
| url | http://europepmc.org/articles/PMC5082853?pdf=render |
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