A Comparative Study of Chemical Profiling and Bioactivities between Thai and Foreign Hemp Seed Species (<i>Cannabis sativa</i> L.) Plus an In-Silico Investigation
Hemp (<i>Cannabis sativa</i> L.) is a plant widely used by humans for textiles, food, and medicine. Thus, this study aimed to characterize the chemical profiling of 12 hemp seed extracts from Thai (HS-TH) and foreign (HS-FS) samples using gas chromatography-mass spectrometry (GC–MS). The...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/13/1/55 |
| _version_ | 1797358820951851008 |
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| author | Suthinee Sangkanu Thanet Pitakbut Sathianpong Phoopha Jiraporn Khanansuk Kasemsiri Chandarajoti Sukanya Dej-adisai |
| author_facet | Suthinee Sangkanu Thanet Pitakbut Sathianpong Phoopha Jiraporn Khanansuk Kasemsiri Chandarajoti Sukanya Dej-adisai |
| author_sort | Suthinee Sangkanu |
| collection | DOAJ |
| description | Hemp (<i>Cannabis sativa</i> L.) is a plant widely used by humans for textiles, food, and medicine. Thus, this study aimed to characterize the chemical profiling of 12 hemp seed extracts from Thai (HS-TH) and foreign (HS-FS) samples using gas chromatography-mass spectrometry (GC–MS). Their antibacterial activity and α-glucosidase inhibitory activity were assayed. Linoleic acid (17.63–86.53%) was a major component presented in Thai hemp seed extracts, while α,β-gluco-octonic acid lactone (30.39%), clionasterol (13.42–29.07%), and glyceryl-linoleate (15.12%) were detected as the main metabolites found in foreign hemp seed extracts. Furthermore, eight extracts from both Thai and foreign hemp seed exhibited antibacterial activity against <i>Staphylococcus aureus</i>, <i>Staphylococcus epidermidis</i>, Methicillin-resistant <i>Staphylococcus aureus</i>, and <i>Cutibacterium acnes</i>, with MIC values ranging from 128 to 2048 µg/mL. Interestingly, the ethanol extract of Thai hemp seed (HS-TH-2-M-E) showed superior α-glucosidase inhibition (IC<sub>50</sub> value of 33.27 ug/mL) over foreign species. The combination between Thai hemp species (HS-TH-2-M-E) and acarbose showed a synergistic effect against α-glucosidase. Furthermore, the docking investigation revealed that fatty acids had a greater impact on α-glucosidase than fatty acid esters and cannabinoids. The computational simulation predicts a potential allosteric binding pocket of guanosine on glucosidase and is the first description of gluco-octonic acid’s anti-glucosidase activity in silico. The findings concluded that Thai hemp seed could be used as a resource for supplemental drugs or dietary therapy for diabetes mellitus. |
| first_indexed | 2024-03-08T15:07:46Z |
| format | Article |
| id | doaj.art-bc03c1eed4c64fd494cb4153d9ae89eb |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-08T15:07:46Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-bc03c1eed4c64fd494cb4153d9ae89eb2024-01-10T14:56:46ZengMDPI AGFoods2304-81582023-12-011315510.3390/foods13010055A Comparative Study of Chemical Profiling and Bioactivities between Thai and Foreign Hemp Seed Species (<i>Cannabis sativa</i> L.) Plus an In-Silico InvestigationSuthinee Sangkanu0Thanet Pitakbut1Sathianpong Phoopha2Jiraporn Khanansuk3Kasemsiri Chandarajoti4Sukanya Dej-adisai5Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla 90112, ThailandPharmaceutical Biology, Department of Biology, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), 91058 Erlangen, GermanyTraditional Thai Medical Research and Innovation Center, Faculty of Traditional Thai Medicine, Prince of Songkla University, Hat Yai, Songkhla 90112, ThailandDepartment of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla 90112, ThailandDepartment of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla 90112, ThailandDepartment of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat Yai, Songkhla 90112, ThailandHemp (<i>Cannabis sativa</i> L.) is a plant widely used by humans for textiles, food, and medicine. Thus, this study aimed to characterize the chemical profiling of 12 hemp seed extracts from Thai (HS-TH) and foreign (HS-FS) samples using gas chromatography-mass spectrometry (GC–MS). Their antibacterial activity and α-glucosidase inhibitory activity were assayed. Linoleic acid (17.63–86.53%) was a major component presented in Thai hemp seed extracts, while α,β-gluco-octonic acid lactone (30.39%), clionasterol (13.42–29.07%), and glyceryl-linoleate (15.12%) were detected as the main metabolites found in foreign hemp seed extracts. Furthermore, eight extracts from both Thai and foreign hemp seed exhibited antibacterial activity against <i>Staphylococcus aureus</i>, <i>Staphylococcus epidermidis</i>, Methicillin-resistant <i>Staphylococcus aureus</i>, and <i>Cutibacterium acnes</i>, with MIC values ranging from 128 to 2048 µg/mL. Interestingly, the ethanol extract of Thai hemp seed (HS-TH-2-M-E) showed superior α-glucosidase inhibition (IC<sub>50</sub> value of 33.27 ug/mL) over foreign species. The combination between Thai hemp species (HS-TH-2-M-E) and acarbose showed a synergistic effect against α-glucosidase. Furthermore, the docking investigation revealed that fatty acids had a greater impact on α-glucosidase than fatty acid esters and cannabinoids. The computational simulation predicts a potential allosteric binding pocket of guanosine on glucosidase and is the first description of gluco-octonic acid’s anti-glucosidase activity in silico. The findings concluded that Thai hemp seed could be used as a resource for supplemental drugs or dietary therapy for diabetes mellitus.https://www.mdpi.com/2304-8158/13/1/55<i>Cannabis sativa</i>hemp seedantibacterial activityα-glucosidase activityanti-diabeteschemical profiling |
| spellingShingle | Suthinee Sangkanu Thanet Pitakbut Sathianpong Phoopha Jiraporn Khanansuk Kasemsiri Chandarajoti Sukanya Dej-adisai A Comparative Study of Chemical Profiling and Bioactivities between Thai and Foreign Hemp Seed Species (<i>Cannabis sativa</i> L.) Plus an In-Silico Investigation Foods <i>Cannabis sativa</i> hemp seed antibacterial activity α-glucosidase activity anti-diabetes chemical profiling |
| title | A Comparative Study of Chemical Profiling and Bioactivities between Thai and Foreign Hemp Seed Species (<i>Cannabis sativa</i> L.) Plus an In-Silico Investigation |
| title_full | A Comparative Study of Chemical Profiling and Bioactivities between Thai and Foreign Hemp Seed Species (<i>Cannabis sativa</i> L.) Plus an In-Silico Investigation |
| title_fullStr | A Comparative Study of Chemical Profiling and Bioactivities between Thai and Foreign Hemp Seed Species (<i>Cannabis sativa</i> L.) Plus an In-Silico Investigation |
| title_full_unstemmed | A Comparative Study of Chemical Profiling and Bioactivities between Thai and Foreign Hemp Seed Species (<i>Cannabis sativa</i> L.) Plus an In-Silico Investigation |
| title_short | A Comparative Study of Chemical Profiling and Bioactivities between Thai and Foreign Hemp Seed Species (<i>Cannabis sativa</i> L.) Plus an In-Silico Investigation |
| title_sort | comparative study of chemical profiling and bioactivities between thai and foreign hemp seed species i cannabis sativa i l plus an in silico investigation |
| topic | <i>Cannabis sativa</i> hemp seed antibacterial activity α-glucosidase activity anti-diabetes chemical profiling |
| url | https://www.mdpi.com/2304-8158/13/1/55 |
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