Derivation of LIF-independent mouse iPS cells with modified Oct4
It has been very difficult, if not impossible, to establish mouse induced pluripotent stem cells (iPSCs) from differentiated cells, such as fibroblasts, without leukemia inhibitory factor (LIF). We have established and maintained LIF-independent iPSCs for longer than 120 days with modified Oct4 alon...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2015-09-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506115001087 |
| _version_ | 1818110868643119104 |
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| author | Hiroyuki Hirai Meri Firpo Nobuaki Kikyo |
| author_facet | Hiroyuki Hirai Meri Firpo Nobuaki Kikyo |
| author_sort | Hiroyuki Hirai |
| collection | DOAJ |
| description | It has been very difficult, if not impossible, to establish mouse induced pluripotent stem cells (iPSCs) from differentiated cells, such as fibroblasts, without leukemia inhibitory factor (LIF). We have established and maintained LIF-independent iPSCs for longer than 120 days with modified Oct4 along with Sox2, Klf4, and c-Myc. The iPSCs will provide a novel tool to investigate the roles of the LIF-Stat3 signaling pathway in mouse pluripotent stem cells. |
| first_indexed | 2024-12-11T02:53:59Z |
| format | Article |
| id | doaj.art-bc046d0bee7046398db2b3d33595260b |
| institution | Directory Open Access Journal |
| issn | 1873-5061 1876-7753 |
| language | English |
| last_indexed | 2024-12-11T02:53:59Z |
| publishDate | 2015-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-bc046d0bee7046398db2b3d33595260b2022-12-22T01:23:13ZengElsevierStem Cell Research1873-50611876-77532015-09-0115238438610.1016/j.scr.2015.08.005Derivation of LIF-independent mouse iPS cells with modified Oct4Hiroyuki Hirai0Meri Firpo1Nobuaki Kikyo2Stem Cell Institute, University of Minnesota, Minneapolis, MN 55455, USAStem Cell Institute, University of Minnesota, Minneapolis, MN 55455, USAStem Cell Institute, University of Minnesota, Minneapolis, MN 55455, USAIt has been very difficult, if not impossible, to establish mouse induced pluripotent stem cells (iPSCs) from differentiated cells, such as fibroblasts, without leukemia inhibitory factor (LIF). We have established and maintained LIF-independent iPSCs for longer than 120 days with modified Oct4 along with Sox2, Klf4, and c-Myc. The iPSCs will provide a novel tool to investigate the roles of the LIF-Stat3 signaling pathway in mouse pluripotent stem cells.http://www.sciencedirect.com/science/article/pii/S1873506115001087 |
| spellingShingle | Hiroyuki Hirai Meri Firpo Nobuaki Kikyo Derivation of LIF-independent mouse iPS cells with modified Oct4 Stem Cell Research |
| title | Derivation of LIF-independent mouse iPS cells with modified Oct4 |
| title_full | Derivation of LIF-independent mouse iPS cells with modified Oct4 |
| title_fullStr | Derivation of LIF-independent mouse iPS cells with modified Oct4 |
| title_full_unstemmed | Derivation of LIF-independent mouse iPS cells with modified Oct4 |
| title_short | Derivation of LIF-independent mouse iPS cells with modified Oct4 |
| title_sort | derivation of lif independent mouse ips cells with modified oct4 |
| url | http://www.sciencedirect.com/science/article/pii/S1873506115001087 |
| work_keys_str_mv | AT hiroyukihirai derivationoflifindependentmouseipscellswithmodifiedoct4 AT merifirpo derivationoflifindependentmouseipscellswithmodifiedoct4 AT nobuakikikyo derivationoflifindependentmouseipscellswithmodifiedoct4 |