Brain Derived Neurotrophic Factor and Superior Collicular Extract Regulate the Expression of the 1.6 Subfamily of Voltage-gated Potassium Channels in the Developing Rat Retina in vitro
Purpose: To evaluate the role of brain derived neurotrophic factor (BDNF) and superior collicular extract (SCE) on the expression of the 1.6 subfamily of voltagegated potassium channels (VG Kv 1.6 channels) in retinal ganglion cells (RGCs) of rats in an in vitro model. Methods: Neonatal retinal cul...
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Knowledge E
2012-01-01
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| Series: | Journal of Ophthalmic & Vision Research |
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| Online Access: | http://www.jovr.org/article.asp?issn=2008-322X;year=2012;volume=7;issue=2;spage=139;epage=147;aulast=Golla |
| _version_ | 1797991368438579200 |
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| author | Kavita Marita Golla Trichur Ramaswamy Raju Sumanthra Chatterji |
| author_facet | Kavita Marita Golla Trichur Ramaswamy Raju Sumanthra Chatterji |
| author_sort | Kavita Marita Golla |
| collection | DOAJ |
| description | Purpose: To evaluate the role of brain derived neurotrophic factor (BDNF) and superior collicular extract (SCE) on the expression of the 1.6 subfamily of voltagegated potassium channels (VG Kv 1.6 channels) in retinal ganglion cells (RGCs) of rats in an in vitro model.
Methods: Neonatal retinal cultures were supplemented with trophic factors of interest, namely BDNF and SCE, at 0 DIV (days in vitro), 6 DIV and both 0 and 6 DIV. The expression of VG Kv 1.6 channels was evaluated by immunostaining with anti Kv 1.6 and immunofluorescence was measured by confocal scanning laser microscopy on 4, 6, 8, 10 and 12 DIV. The immunofluorescence indirectly measured the quantity of ion channels being expressed.
Results: RGCs were identified by their soma size. BDNF and SCE enhanced RGC survival by enhancing extensive neurite outgrowth, and increased the expression of VG Kv 1.6 channels; the effect of SCE was more significant than BDNF. Trophic factors also enhanced the survival of RGCs by increasing the expression of ion channels thereby contributing to spontaneous bursts of action potentials in the early stages of RGC development.
Conclusion: The expression of delayed rectifier VG Kv 1.6 channels in RGCs may determine membrane excitability and responsiveness to trophic factors, this plays a key role in the refinement of developing retinal circuits. |
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| id | doaj.art-bc04920d2e46465fa3c6e009d7468c67 |
| institution | Directory Open Access Journal |
| issn | 2008-322X |
| language | English |
| last_indexed | 2024-04-11T08:51:15Z |
| publishDate | 2012-01-01 |
| publisher | Knowledge E |
| record_format | Article |
| series | Journal of Ophthalmic & Vision Research |
| spelling | doaj.art-bc04920d2e46465fa3c6e009d7468c672022-12-22T04:33:35ZengKnowledge EJournal of Ophthalmic & Vision Research2008-322X2012-01-0172139147Brain Derived Neurotrophic Factor and Superior Collicular Extract Regulate the Expression of the 1.6 Subfamily of Voltage-gated Potassium Channels in the Developing Rat Retina in vitroKavita Marita GollaTrichur Ramaswamy RajuSumanthra ChatterjiPurpose: To evaluate the role of brain derived neurotrophic factor (BDNF) and superior collicular extract (SCE) on the expression of the 1.6 subfamily of voltagegated potassium channels (VG Kv 1.6 channels) in retinal ganglion cells (RGCs) of rats in an in vitro model. Methods: Neonatal retinal cultures were supplemented with trophic factors of interest, namely BDNF and SCE, at 0 DIV (days in vitro), 6 DIV and both 0 and 6 DIV. The expression of VG Kv 1.6 channels was evaluated by immunostaining with anti Kv 1.6 and immunofluorescence was measured by confocal scanning laser microscopy on 4, 6, 8, 10 and 12 DIV. The immunofluorescence indirectly measured the quantity of ion channels being expressed. Results: RGCs were identified by their soma size. BDNF and SCE enhanced RGC survival by enhancing extensive neurite outgrowth, and increased the expression of VG Kv 1.6 channels; the effect of SCE was more significant than BDNF. Trophic factors also enhanced the survival of RGCs by increasing the expression of ion channels thereby contributing to spontaneous bursts of action potentials in the early stages of RGC development. Conclusion: The expression of delayed rectifier VG Kv 1.6 channels in RGCs may determine membrane excitability and responsiveness to trophic factors, this plays a key role in the refinement of developing retinal circuits.http://www.jovr.org/article.asp?issn=2008-322X;year=2012;volume=7;issue=2;spage=139;epage=147;aulast=GollaBrain Derived Neurotrophic Factor; Superior Collicular Extract; Retinal Ganglion Cells; Potassium Channels |
| spellingShingle | Kavita Marita Golla Trichur Ramaswamy Raju Sumanthra Chatterji Brain Derived Neurotrophic Factor and Superior Collicular Extract Regulate the Expression of the 1.6 Subfamily of Voltage-gated Potassium Channels in the Developing Rat Retina in vitro Journal of Ophthalmic & Vision Research Brain Derived Neurotrophic Factor; Superior Collicular Extract; Retinal Ganglion Cells; Potassium Channels |
| title | Brain Derived Neurotrophic Factor and Superior Collicular Extract Regulate the Expression of the 1.6 Subfamily of Voltage-gated Potassium Channels in the Developing Rat Retina in vitro |
| title_full | Brain Derived Neurotrophic Factor and Superior Collicular Extract Regulate the Expression of the 1.6 Subfamily of Voltage-gated Potassium Channels in the Developing Rat Retina in vitro |
| title_fullStr | Brain Derived Neurotrophic Factor and Superior Collicular Extract Regulate the Expression of the 1.6 Subfamily of Voltage-gated Potassium Channels in the Developing Rat Retina in vitro |
| title_full_unstemmed | Brain Derived Neurotrophic Factor and Superior Collicular Extract Regulate the Expression of the 1.6 Subfamily of Voltage-gated Potassium Channels in the Developing Rat Retina in vitro |
| title_short | Brain Derived Neurotrophic Factor and Superior Collicular Extract Regulate the Expression of the 1.6 Subfamily of Voltage-gated Potassium Channels in the Developing Rat Retina in vitro |
| title_sort | brain derived neurotrophic factor and superior collicular extract regulate the expression of the 1 6 subfamily of voltage gated potassium channels in the developing rat retina in vitro |
| topic | Brain Derived Neurotrophic Factor; Superior Collicular Extract; Retinal Ganglion Cells; Potassium Channels |
| url | http://www.jovr.org/article.asp?issn=2008-322X;year=2012;volume=7;issue=2;spage=139;epage=147;aulast=Golla |
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