Protective Effects of Polyphenol Enriched Complex Plants Extract on Metabolic Dysfunctions Associated with Obesity and Related Nonalcoholic Fatty Liver Diseases in High Fat Diet-Induced C57BL/6 Mice
Background: Currently, obesity is a global health challenge due to its increasing prevalence and associated health risk. It is associated with various metabolic diseases, including diabetes, hypertension, cardiovascular disease, stroke, certain forms of cancer, and non-alcoholic liver diseases (NAFL...
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MDPI AG
2021-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/2/302 |
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| author | Ahtesham Hussain Jin Sook Cho Jong-Seok Kim Young Ik Lee |
| author_facet | Ahtesham Hussain Jin Sook Cho Jong-Seok Kim Young Ik Lee |
| author_sort | Ahtesham Hussain |
| collection | DOAJ |
| description | Background: Currently, obesity is a global health challenge due to its increasing prevalence and associated health risk. It is associated with various metabolic diseases, including diabetes, hypertension, cardiovascular disease, stroke, certain forms of cancer, and non-alcoholic liver diseases (NAFLD). Objective: The aim of this study to evaluate the effects of polyphenol enriched herbal complex (<i>Rubus crataegifolius</i>/ellagic acid, <i>Crataegus pinnatifida</i> Bunge/vitexin, chlorogenic acid, <i>Cinnamomum cassiaa</i>/cinnamic acid) on obesity and obesity induced NAFLD in the high-fat diet (HFD)-induced obese mouse model. Methods: Obesity was induced in male C57BL/6 mice using HFD. After 8 weeks, the mice were treated with HFD+ plants extract for 8 weeks. Body weight, food intake weekly, and blood sugar level were measured. After sacrifice, changes in the treated group’s liver weight, fat weight, serum biochemical parameters, hormone levels, and enzyme levels were measured. For histological analysis, tissues were stained with hematoxylin-eosin (H&E) and Oil Red-O. Results: Our results showed that the herbal complex ameliorated body weight and liver weight gain, and decreased total body fat in HFD-fed animals. Post prandial blood glucose (PBG) and fasting blood glucose (FBG) were lower in the herbal complex-treated group than in the HFD control group. Additionally, herbal formulation treatment significantly increased HDL levels in serum and decreased TC, TG, AST, ALT, deposition of fat droplets in the liver, and intima media thickness (IMT) in the aorta. Herbal complex increased serum adiponectin and decreased serum leptin. Herbal complex also increased carnitine palmityl transferase (CPT) activity and significantly decreased enzyme activity of beta-hydroxy beta methyl glutamyl-CoA (HMG-CoA) reductase, and fatty acid synthase (FAS). Conclusions: The results of this study demonstrated that the herbal complex is an effective herbal formulation in the attenuation of obesity and obesity-induced metabolic dysfunction including NAFLD in HFD-induced mouse model. |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T05:30:34Z |
| publishDate | 2021-01-01 |
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| series | Molecules |
| spelling | doaj.art-bc05362b2abe47c1aec628c92924d7a92023-12-03T12:33:00ZengMDPI AGMolecules1420-30492021-01-0126230210.3390/molecules26020302Protective Effects of Polyphenol Enriched Complex Plants Extract on Metabolic Dysfunctions Associated with Obesity and Related Nonalcoholic Fatty Liver Diseases in High Fat Diet-Induced C57BL/6 MiceAhtesham Hussain0Jin Sook Cho1Jong-Seok Kim2Young Ik Lee3Lee’s Biotech Co., Ltd. #416, C Dong, 17 Techno 4-ro, Yuseon-gu, Daejeon 34013, KoreaLee’s Biotech Co., Ltd. #416, C Dong, 17 Techno 4-ro, Yuseon-gu, Daejeon 34013, KoreaMyunggok Medical Research Institute, College of Medicine, Konyang University, Daejeon 35365, KoreaLee’s Biotech Co., Ltd. #416, C Dong, 17 Techno 4-ro, Yuseon-gu, Daejeon 34013, KoreaBackground: Currently, obesity is a global health challenge due to its increasing prevalence and associated health risk. It is associated with various metabolic diseases, including diabetes, hypertension, cardiovascular disease, stroke, certain forms of cancer, and non-alcoholic liver diseases (NAFLD). Objective: The aim of this study to evaluate the effects of polyphenol enriched herbal complex (<i>Rubus crataegifolius</i>/ellagic acid, <i>Crataegus pinnatifida</i> Bunge/vitexin, chlorogenic acid, <i>Cinnamomum cassiaa</i>/cinnamic acid) on obesity and obesity induced NAFLD in the high-fat diet (HFD)-induced obese mouse model. Methods: Obesity was induced in male C57BL/6 mice using HFD. After 8 weeks, the mice were treated with HFD+ plants extract for 8 weeks. Body weight, food intake weekly, and blood sugar level were measured. After sacrifice, changes in the treated group’s liver weight, fat weight, serum biochemical parameters, hormone levels, and enzyme levels were measured. For histological analysis, tissues were stained with hematoxylin-eosin (H&E) and Oil Red-O. Results: Our results showed that the herbal complex ameliorated body weight and liver weight gain, and decreased total body fat in HFD-fed animals. Post prandial blood glucose (PBG) and fasting blood glucose (FBG) were lower in the herbal complex-treated group than in the HFD control group. Additionally, herbal formulation treatment significantly increased HDL levels in serum and decreased TC, TG, AST, ALT, deposition of fat droplets in the liver, and intima media thickness (IMT) in the aorta. Herbal complex increased serum adiponectin and decreased serum leptin. Herbal complex also increased carnitine palmityl transferase (CPT) activity and significantly decreased enzyme activity of beta-hydroxy beta methyl glutamyl-CoA (HMG-CoA) reductase, and fatty acid synthase (FAS). Conclusions: The results of this study demonstrated that the herbal complex is an effective herbal formulation in the attenuation of obesity and obesity-induced metabolic dysfunction including NAFLD in HFD-induced mouse model.https://www.mdpi.com/1420-3049/26/2/302plants extract<i>Rubus crataegifolius</i><i>Crataegus pinnatifida</i> Bunge<i>Cinnamomum cassia</i>obesitymetabolic dysfunctions |
| spellingShingle | Ahtesham Hussain Jin Sook Cho Jong-Seok Kim Young Ik Lee Protective Effects of Polyphenol Enriched Complex Plants Extract on Metabolic Dysfunctions Associated with Obesity and Related Nonalcoholic Fatty Liver Diseases in High Fat Diet-Induced C57BL/6 Mice Molecules plants extract <i>Rubus crataegifolius</i> <i>Crataegus pinnatifida</i> Bunge <i>Cinnamomum cassia</i> obesity metabolic dysfunctions |
| title | Protective Effects of Polyphenol Enriched Complex Plants Extract on Metabolic Dysfunctions Associated with Obesity and Related Nonalcoholic Fatty Liver Diseases in High Fat Diet-Induced C57BL/6 Mice |
| title_full | Protective Effects of Polyphenol Enriched Complex Plants Extract on Metabolic Dysfunctions Associated with Obesity and Related Nonalcoholic Fatty Liver Diseases in High Fat Diet-Induced C57BL/6 Mice |
| title_fullStr | Protective Effects of Polyphenol Enriched Complex Plants Extract on Metabolic Dysfunctions Associated with Obesity and Related Nonalcoholic Fatty Liver Diseases in High Fat Diet-Induced C57BL/6 Mice |
| title_full_unstemmed | Protective Effects of Polyphenol Enriched Complex Plants Extract on Metabolic Dysfunctions Associated with Obesity and Related Nonalcoholic Fatty Liver Diseases in High Fat Diet-Induced C57BL/6 Mice |
| title_short | Protective Effects of Polyphenol Enriched Complex Plants Extract on Metabolic Dysfunctions Associated with Obesity and Related Nonalcoholic Fatty Liver Diseases in High Fat Diet-Induced C57BL/6 Mice |
| title_sort | protective effects of polyphenol enriched complex plants extract on metabolic dysfunctions associated with obesity and related nonalcoholic fatty liver diseases in high fat diet induced c57bl 6 mice |
| topic | plants extract <i>Rubus crataegifolius</i> <i>Crataegus pinnatifida</i> Bunge <i>Cinnamomum cassia</i> obesity metabolic dysfunctions |
| url | https://www.mdpi.com/1420-3049/26/2/302 |
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