Conformational antibody binding to a native, cell-free expressed GPCR in block copolymer membranes.
G-protein coupled receptors (GPCRs) play a key role in physiological processes and are attractive drug targets. Their biophysical characterization is, however, highly challenging because of their innate instability outside a stabilizing membrane and the difficulty of finding a suitable expression sy...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4203850?pdf=render |
| _version_ | 1818887158478405632 |
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| author | Hans-Peter M de Hoog Esther M Lin JieRong Sourabh Banerjee Fabien M Décaillot Madhavan Nallani |
| author_facet | Hans-Peter M de Hoog Esther M Lin JieRong Sourabh Banerjee Fabien M Décaillot Madhavan Nallani |
| author_sort | Hans-Peter M de Hoog |
| collection | DOAJ |
| description | G-protein coupled receptors (GPCRs) play a key role in physiological processes and are attractive drug targets. Their biophysical characterization is, however, highly challenging because of their innate instability outside a stabilizing membrane and the difficulty of finding a suitable expression system. We here show the cell-free expression of a GPCR, CXCR4, and its direct embedding in diblock copolymer membranes. The polymer-stabilized CXCR4 is readily immobilized onto biosensor chips for label-free binding analysis. Kinetic characterization using a conformationally sensitive antibody shows the receptor to exist in the correctly folded conformation, showing binding behaviour that is commensurate with heterologously expressed CXCR4. |
| first_indexed | 2024-12-19T16:32:47Z |
| format | Article |
| id | doaj.art-bc06f137ef4d4dbdb2c7d4897d0b7bb7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T16:32:47Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-bc06f137ef4d4dbdb2c7d4897d0b7bb72022-12-21T20:14:06ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01910e11084710.1371/journal.pone.0110847Conformational antibody binding to a native, cell-free expressed GPCR in block copolymer membranes.Hans-Peter M de HoogEsther M Lin JieRongSourabh BanerjeeFabien M DécaillotMadhavan NallaniG-protein coupled receptors (GPCRs) play a key role in physiological processes and are attractive drug targets. Their biophysical characterization is, however, highly challenging because of their innate instability outside a stabilizing membrane and the difficulty of finding a suitable expression system. We here show the cell-free expression of a GPCR, CXCR4, and its direct embedding in diblock copolymer membranes. The polymer-stabilized CXCR4 is readily immobilized onto biosensor chips for label-free binding analysis. Kinetic characterization using a conformationally sensitive antibody shows the receptor to exist in the correctly folded conformation, showing binding behaviour that is commensurate with heterologously expressed CXCR4.http://europepmc.org/articles/PMC4203850?pdf=render |
| spellingShingle | Hans-Peter M de Hoog Esther M Lin JieRong Sourabh Banerjee Fabien M Décaillot Madhavan Nallani Conformational antibody binding to a native, cell-free expressed GPCR in block copolymer membranes. PLoS ONE |
| title | Conformational antibody binding to a native, cell-free expressed GPCR in block copolymer membranes. |
| title_full | Conformational antibody binding to a native, cell-free expressed GPCR in block copolymer membranes. |
| title_fullStr | Conformational antibody binding to a native, cell-free expressed GPCR in block copolymer membranes. |
| title_full_unstemmed | Conformational antibody binding to a native, cell-free expressed GPCR in block copolymer membranes. |
| title_short | Conformational antibody binding to a native, cell-free expressed GPCR in block copolymer membranes. |
| title_sort | conformational antibody binding to a native cell free expressed gpcr in block copolymer membranes |
| url | http://europepmc.org/articles/PMC4203850?pdf=render |
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