Molecular and Photosynthetic Performance in the Yellow Leaf Mutant of <i>Torreya grandis</i> According to Transcriptome Sequencing, Chlorophyll <i>a</i> Fluorescence, and Modulated 820 nm Reflection
To study the photosynthetic energy mechanism and electron transfer in yellow leaves, transcriptomics combined with physiological approaches was used to explore the mechanism of the yellow leaf mutant <i>Torreya grandis</i> ‘Merrillii’. The results showed that chlorophyll content, the max...
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MDPI AG
2022-01-01
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| author | Jianshuang Shen Xueqin Li Xiangtao Zhu Zhicheng Ding Xiaoling Huang Xia Chen Songheng Jin |
| author_facet | Jianshuang Shen Xueqin Li Xiangtao Zhu Zhicheng Ding Xiaoling Huang Xia Chen Songheng Jin |
| author_sort | Jianshuang Shen |
| collection | DOAJ |
| description | To study the photosynthetic energy mechanism and electron transfer in yellow leaves, transcriptomics combined with physiological approaches was used to explore the mechanism of the yellow leaf mutant <i>Torreya grandis</i> ‘Merrillii’. The results showed that chlorophyll content, the maximal photochemical efficiency of PSII (<i>F<sub>v</sub>/F<sub>m</sub></i>), and the parameters related to the OJ phase of fluorescence (φ<sub>Eo</sub>, φ<sub>Ro</sub>) were all decreased significantly in mutant-type <i>T. grandis</i> leaves. The efficiency needed for an electron to be transferred from the reduced carriers between the two photosystems to the end acceptors of the PSI (δ<sub>Ro</sub>) and the quantum yield of the energy dissipation (φ<sub>Do</sub>) were higher in the leaves of mutant-type <i>T. grandis</i> compared to those in wild-type leaves. Analysis of the prompt fluorescence kinetics and modulated 820 nm reflection showed that the electron transfer of PSII was decreased, and PSI activity was increased in yellow <i>T</i><i>. grandis</i> leaves. Transcriptome data showed that the unigenes involved in chlorophyll synthesis and the photosynthetic electron transport complex were downregulated in the leaves of mutant-type <i>T. grandis</i> compared to wild-type leaves, while there were no observable changes in carotenoid content and biosynthesis. These findings suggest that the downregulation of genes involved in chlorophyll synthesis leads to decreased chlorophyll content, resulting in both PSI activity and carotenoids having higher tolerance when acting as photo-protective mechanisms for coping with chlorophyll deficit and decrease in linear electron transport in PSII. |
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| language | English |
| last_indexed | 2024-03-10T00:04:38Z |
| publishDate | 2022-01-01 |
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| spelling | doaj.art-bc08767c3b8f4b1eaf119c7cf35f53b52023-11-23T16:11:46ZengMDPI AGCells2073-44092022-01-0111343110.3390/cells11030431Molecular and Photosynthetic Performance in the Yellow Leaf Mutant of <i>Torreya grandis</i> According to Transcriptome Sequencing, Chlorophyll <i>a</i> Fluorescence, and Modulated 820 nm ReflectionJianshuang Shen0Xueqin Li1Xiangtao Zhu2Zhicheng Ding3Xiaoling Huang4Xia Chen5Songheng Jin6Jiyang College, Zhejiang A&F University, Zhuji 311800, ChinaJiyang College, Zhejiang A&F University, Zhuji 311800, ChinaJiyang College, Zhejiang A&F University, Zhuji 311800, ChinaJiyang College, Zhejiang A&F University, Zhuji 311800, ChinaJiyang College, Zhejiang A&F University, Zhuji 311800, ChinaJiyang College, Zhejiang A&F University, Zhuji 311800, ChinaJiyang College, Zhejiang A&F University, Zhuji 311800, ChinaTo study the photosynthetic energy mechanism and electron transfer in yellow leaves, transcriptomics combined with physiological approaches was used to explore the mechanism of the yellow leaf mutant <i>Torreya grandis</i> ‘Merrillii’. The results showed that chlorophyll content, the maximal photochemical efficiency of PSII (<i>F<sub>v</sub>/F<sub>m</sub></i>), and the parameters related to the OJ phase of fluorescence (φ<sub>Eo</sub>, φ<sub>Ro</sub>) were all decreased significantly in mutant-type <i>T. grandis</i> leaves. The efficiency needed for an electron to be transferred from the reduced carriers between the two photosystems to the end acceptors of the PSI (δ<sub>Ro</sub>) and the quantum yield of the energy dissipation (φ<sub>Do</sub>) were higher in the leaves of mutant-type <i>T. grandis</i> compared to those in wild-type leaves. Analysis of the prompt fluorescence kinetics and modulated 820 nm reflection showed that the electron transfer of PSII was decreased, and PSI activity was increased in yellow <i>T</i><i>. grandis</i> leaves. Transcriptome data showed that the unigenes involved in chlorophyll synthesis and the photosynthetic electron transport complex were downregulated in the leaves of mutant-type <i>T. grandis</i> compared to wild-type leaves, while there were no observable changes in carotenoid content and biosynthesis. These findings suggest that the downregulation of genes involved in chlorophyll synthesis leads to decreased chlorophyll content, resulting in both PSI activity and carotenoids having higher tolerance when acting as photo-protective mechanisms for coping with chlorophyll deficit and decrease in linear electron transport in PSII.https://www.mdpi.com/2073-4409/11/3/431comparative transcriptomephotosynthetic electron transportyellow leaf color mutantgene expressionphotosynthesis |
| spellingShingle | Jianshuang Shen Xueqin Li Xiangtao Zhu Zhicheng Ding Xiaoling Huang Xia Chen Songheng Jin Molecular and Photosynthetic Performance in the Yellow Leaf Mutant of <i>Torreya grandis</i> According to Transcriptome Sequencing, Chlorophyll <i>a</i> Fluorescence, and Modulated 820 nm Reflection Cells comparative transcriptome photosynthetic electron transport yellow leaf color mutant gene expression photosynthesis |
| title | Molecular and Photosynthetic Performance in the Yellow Leaf Mutant of <i>Torreya grandis</i> According to Transcriptome Sequencing, Chlorophyll <i>a</i> Fluorescence, and Modulated 820 nm Reflection |
| title_full | Molecular and Photosynthetic Performance in the Yellow Leaf Mutant of <i>Torreya grandis</i> According to Transcriptome Sequencing, Chlorophyll <i>a</i> Fluorescence, and Modulated 820 nm Reflection |
| title_fullStr | Molecular and Photosynthetic Performance in the Yellow Leaf Mutant of <i>Torreya grandis</i> According to Transcriptome Sequencing, Chlorophyll <i>a</i> Fluorescence, and Modulated 820 nm Reflection |
| title_full_unstemmed | Molecular and Photosynthetic Performance in the Yellow Leaf Mutant of <i>Torreya grandis</i> According to Transcriptome Sequencing, Chlorophyll <i>a</i> Fluorescence, and Modulated 820 nm Reflection |
| title_short | Molecular and Photosynthetic Performance in the Yellow Leaf Mutant of <i>Torreya grandis</i> According to Transcriptome Sequencing, Chlorophyll <i>a</i> Fluorescence, and Modulated 820 nm Reflection |
| title_sort | molecular and photosynthetic performance in the yellow leaf mutant of i torreya grandis i according to transcriptome sequencing chlorophyll i a i fluorescence and modulated 820 nm reflection |
| topic | comparative transcriptome photosynthetic electron transport yellow leaf color mutant gene expression photosynthesis |
| url | https://www.mdpi.com/2073-4409/11/3/431 |
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