Evaluation of the antidiabetic potential of an isolated hydroalcoholic fraction from the fruit of withania coagulans
The hydro-alcoholic extract of Withania coagulans fruits was investigated for preliminary phytochemical screening and characterized by high-performance thin-layer chromatography. Column chromatography of the hydro-alcoholic extract of W. coagulans eluted with four different combinations of ethyl ace...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2021-01-01
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| Series: | Journal of Pharmacy and Bioallied Sciences |
| Subjects: | |
| Online Access: | http://www.jpbsonline.org/article.asp?issn=0975-7406;year=2021;volume=13;issue=4;spage=367;epage=372;aulast=Waris |
| _version_ | 1828105432515739648 |
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| author | Md Waris Naiyer Shahzad Saeed Saeed Al-Ghamdi Showkat Rasool Mir Tanuja |
| author_facet | Md Waris Naiyer Shahzad Saeed Saeed Al-Ghamdi Showkat Rasool Mir Tanuja |
| author_sort | Md Waris |
| collection | DOAJ |
| description | The hydro-alcoholic extract of Withania coagulans fruits was investigated for preliminary phytochemical screening and characterized by high-performance thin-layer chromatography. Column chromatography of the hydro-alcoholic extract of W. coagulans eluted with four different combinations of ethyl acetate and methanol yielded four fractions (WCF01, WCF02, WCF03, and WCF04). One of these fractions, WCF02, significantly (P < 0.05) inhibited in vitro α-amylase and α-glucosidase activity with IC50 values of 104.71 μg/mL and 70.79 μg/mL, respectively. WCF02 further reduced blood glucose levels in comparison to control in the starch tolerance test. The extract showed a relative dose-dependent effect. It was observed that none of the extracts could delay the peak blood glucose that was achieved after 60 min of carbohydrate challenge, but these blunted the glycemic peak. |
| first_indexed | 2024-04-11T10:03:16Z |
| format | Article |
| id | doaj.art-bc0adee0c6df406c9950fc60c60aa218 |
| institution | Directory Open Access Journal |
| issn | 0975-7406 |
| language | English |
| last_indexed | 2024-04-11T10:03:16Z |
| publishDate | 2021-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Pharmacy and Bioallied Sciences |
| spelling | doaj.art-bc0adee0c6df406c9950fc60c60aa2182022-12-22T04:30:19ZengWolters Kluwer Medknow PublicationsJournal of Pharmacy and Bioallied Sciences0975-74062021-01-0113436737210.4103/jpbs.JPBS_423_20Evaluation of the antidiabetic potential of an isolated hydroalcoholic fraction from the fruit of withania coagulansMd WarisNaiyer ShahzadSaeed Saeed Al-GhamdiShowkat Rasool MirTanujaThe hydro-alcoholic extract of Withania coagulans fruits was investigated for preliminary phytochemical screening and characterized by high-performance thin-layer chromatography. Column chromatography of the hydro-alcoholic extract of W. coagulans eluted with four different combinations of ethyl acetate and methanol yielded four fractions (WCF01, WCF02, WCF03, and WCF04). One of these fractions, WCF02, significantly (P < 0.05) inhibited in vitro α-amylase and α-glucosidase activity with IC50 values of 104.71 μg/mL and 70.79 μg/mL, respectively. WCF02 further reduced blood glucose levels in comparison to control in the starch tolerance test. The extract showed a relative dose-dependent effect. It was observed that none of the extracts could delay the peak blood glucose that was achieved after 60 min of carbohydrate challenge, but these blunted the glycemic peak.http://www.jpbsonline.org/article.asp?issn=0975-7406;year=2021;volume=13;issue=4;spage=367;epage=372;aulast=Warisglycemiahigh-performance thin-layer chromatographyhydro-alcoholicwithania coagulansα-amylaseα-glucosidase |
| spellingShingle | Md Waris Naiyer Shahzad Saeed Saeed Al-Ghamdi Showkat Rasool Mir Tanuja Evaluation of the antidiabetic potential of an isolated hydroalcoholic fraction from the fruit of withania coagulans Journal of Pharmacy and Bioallied Sciences glycemia high-performance thin-layer chromatography hydro-alcoholic withania coagulans α-amylase α-glucosidase |
| title | Evaluation of the antidiabetic potential of an isolated hydroalcoholic fraction from the fruit of withania coagulans |
| title_full | Evaluation of the antidiabetic potential of an isolated hydroalcoholic fraction from the fruit of withania coagulans |
| title_fullStr | Evaluation of the antidiabetic potential of an isolated hydroalcoholic fraction from the fruit of withania coagulans |
| title_full_unstemmed | Evaluation of the antidiabetic potential of an isolated hydroalcoholic fraction from the fruit of withania coagulans |
| title_short | Evaluation of the antidiabetic potential of an isolated hydroalcoholic fraction from the fruit of withania coagulans |
| title_sort | evaluation of the antidiabetic potential of an isolated hydroalcoholic fraction from the fruit of withania coagulans |
| topic | glycemia high-performance thin-layer chromatography hydro-alcoholic withania coagulans α-amylase α-glucosidase |
| url | http://www.jpbsonline.org/article.asp?issn=0975-7406;year=2021;volume=13;issue=4;spage=367;epage=372;aulast=Waris |
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