Zingiber officinale extract maximizes the efficacy of simvastatin as a hypolipidemic drug in obese male rats
Abstract Obesity became a serious public health problem with enormous socioeconomic implications among the Egyptian population. The present investigation aimed to explore the efficacy of Zingiber officinale extract as a hypolipidemic agent combined with the commercially well‐known anti‐obesity drug...
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| Format: | Article |
| Language: | English |
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Wiley
2024-03-01
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| Series: | Food Science & Nutrition |
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| Online Access: | https://doi.org/10.1002/fsn3.3889 |
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| author | Moustafa Salaheldin Abdelhamid Mohamed Hassan Sherif Hazem R. Abaza Lamiaa M. M. El‐Maghraby Shimaa H. Watad Ahmed E. Awad |
| author_facet | Moustafa Salaheldin Abdelhamid Mohamed Hassan Sherif Hazem R. Abaza Lamiaa M. M. El‐Maghraby Shimaa H. Watad Ahmed E. Awad |
| author_sort | Moustafa Salaheldin Abdelhamid |
| collection | DOAJ |
| description | Abstract Obesity became a serious public health problem with enormous socioeconomic implications among the Egyptian population. The present investigation aimed to explore the efficacy of Zingiber officinale extract as a hypolipidemic agent combined with the commercially well‐known anti‐obesity drug simvastatin in obese rats. Thirty‐five male Wister rats were randomly divided into five groups as follows: group I received a standard balanced diet for ten weeks; high‐fat diet was orally administered to rats in groups II–V for ten weeks. From the fifth week to the tenth week, group III orally received simvastatin (40 mg/kg B.W.), group IV orally received Z. officinale root extract (400 mg/kg B.W.), and group V orally received simvastatin (20 mg/kg B.W.) plus Z. officinale extract (200 mg/kg B.W.) separately. Liver and kidney function tests, lipid profiles, serum glucose, insulin, and leptin were determined. Quantitative RT‐PCR analysis of PPAR‐γ, iNOS, HMG‐CoA reductase, and GLUT‐4 genes was carried out. Caspase 3 was estimated in liver and kidney tissues immunohistochemically. Liver and kidney tissues were examined histologically. The administration of Z. officinale extract plus simvastatin to high‐fat diet‐fed rats caused a significant reduction in the expression of HMG‐coA reductase and iNOS by 41.81% and 88.05%, respectively, compared to highfat diet (HFD)‐fed rats that received simvastatin only. Otherwise, a significant increase was noticed in the expression of PPAR‐γ and GLUT‐4 by 33.3% and 138.81%, respectively, compared to those that received simvastatin only. Immunohistochemistry emphasized that a combination of Z. officinale extract plus simvastatin significantly suppressed caspase 3 in the hepatic tissue of high‐fat diet‐fed rats. Moreover, the best results of lipid profile indices and hormonal indicators were obtained when rats received Z. officinale extract plus simvastatin. Z. officinale extract enhanced the efficiency of simvastatin as a hypolipidemic drug in obese rats due to the high contents of flavonoid and phenolic ingredients. |
| first_indexed | 2024-03-07T14:12:59Z |
| format | Article |
| id | doaj.art-bc0bc7aedbce4e169ec894bd251068fd |
| institution | Directory Open Access Journal |
| issn | 2048-7177 |
| language | English |
| last_indexed | 2024-03-07T14:12:59Z |
| publishDate | 2024-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Science & Nutrition |
| spelling | doaj.art-bc0bc7aedbce4e169ec894bd251068fd2024-03-06T14:22:28ZengWileyFood Science & Nutrition2048-71772024-03-011231940195410.1002/fsn3.3889Zingiber officinale extract maximizes the efficacy of simvastatin as a hypolipidemic drug in obese male ratsMoustafa Salaheldin Abdelhamid0Mohamed Hassan Sherif1Hazem R. Abaza2Lamiaa M. M. El‐Maghraby3Shimaa H. Watad4Ahmed E. Awad5Biochemistry Department, Faculty of Science Zagazig University Zagazig EgyptChemistry Department, Faculty of Science Zagazig University Zagazig EgyptBiochemistry Department, Faculty of Science Zagazig University Zagazig EgyptAgricultural Biochemistry Department, Faculty of Agriculture Zagazig University Zagazig EgyptBiochemistry Department, Faculty of Science Zagazig University Zagazig EgyptAgricultural Biochemistry Department, Faculty of Agriculture Zagazig University Zagazig EgyptAbstract Obesity became a serious public health problem with enormous socioeconomic implications among the Egyptian population. The present investigation aimed to explore the efficacy of Zingiber officinale extract as a hypolipidemic agent combined with the commercially well‐known anti‐obesity drug simvastatin in obese rats. Thirty‐five male Wister rats were randomly divided into five groups as follows: group I received a standard balanced diet for ten weeks; high‐fat diet was orally administered to rats in groups II–V for ten weeks. From the fifth week to the tenth week, group III orally received simvastatin (40 mg/kg B.W.), group IV orally received Z. officinale root extract (400 mg/kg B.W.), and group V orally received simvastatin (20 mg/kg B.W.) plus Z. officinale extract (200 mg/kg B.W.) separately. Liver and kidney function tests, lipid profiles, serum glucose, insulin, and leptin were determined. Quantitative RT‐PCR analysis of PPAR‐γ, iNOS, HMG‐CoA reductase, and GLUT‐4 genes was carried out. Caspase 3 was estimated in liver and kidney tissues immunohistochemically. Liver and kidney tissues were examined histologically. The administration of Z. officinale extract plus simvastatin to high‐fat diet‐fed rats caused a significant reduction in the expression of HMG‐coA reductase and iNOS by 41.81% and 88.05%, respectively, compared to highfat diet (HFD)‐fed rats that received simvastatin only. Otherwise, a significant increase was noticed in the expression of PPAR‐γ and GLUT‐4 by 33.3% and 138.81%, respectively, compared to those that received simvastatin only. Immunohistochemistry emphasized that a combination of Z. officinale extract plus simvastatin significantly suppressed caspase 3 in the hepatic tissue of high‐fat diet‐fed rats. Moreover, the best results of lipid profile indices and hormonal indicators were obtained when rats received Z. officinale extract plus simvastatin. Z. officinale extract enhanced the efficiency of simvastatin as a hypolipidemic drug in obese rats due to the high contents of flavonoid and phenolic ingredients.https://doi.org/10.1002/fsn3.3889anti‐obesitycaspase‐3GLUT‐4HMG‐CoA reductaseRT‐PCRZingiber officinale |
| spellingShingle | Moustafa Salaheldin Abdelhamid Mohamed Hassan Sherif Hazem R. Abaza Lamiaa M. M. El‐Maghraby Shimaa H. Watad Ahmed E. Awad Zingiber officinale extract maximizes the efficacy of simvastatin as a hypolipidemic drug in obese male rats Food Science & Nutrition anti‐obesity caspase‐3 GLUT‐4 HMG‐CoA reductase RT‐PCR Zingiber officinale |
| title | Zingiber officinale extract maximizes the efficacy of simvastatin as a hypolipidemic drug in obese male rats |
| title_full | Zingiber officinale extract maximizes the efficacy of simvastatin as a hypolipidemic drug in obese male rats |
| title_fullStr | Zingiber officinale extract maximizes the efficacy of simvastatin as a hypolipidemic drug in obese male rats |
| title_full_unstemmed | Zingiber officinale extract maximizes the efficacy of simvastatin as a hypolipidemic drug in obese male rats |
| title_short | Zingiber officinale extract maximizes the efficacy of simvastatin as a hypolipidemic drug in obese male rats |
| title_sort | zingiber officinale extract maximizes the efficacy of simvastatin as a hypolipidemic drug in obese male rats |
| topic | anti‐obesity caspase‐3 GLUT‐4 HMG‐CoA reductase RT‐PCR Zingiber officinale |
| url | https://doi.org/10.1002/fsn3.3889 |
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