Saccharomyces Boulardii Ameliorates Non-alcoholic Steatohepatitis in Mice Induced by a Methionine-Choline-Deficient Diet Through Gut-Liver Axis
Non-alcoholic steatohepatitis (NASH) is affecting people worldwide. Changes in the intestinal microbiome are crucial to NASH. A previous study showed that eradicating intestinal fungi ameliorates NASH; however, the role of intestinal fungi in the development of NASH remains unclear. Saccharomyces bo...
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| Format: | Article |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.887728/full |
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| author | An-Ming Yang An-Ming Yang Chien-Yu Lin Chien-Yu Lin Shih-Hao Liu Guan-Da Syu Guan-Da Syu Hao-Jhe Sun Kuei-Chuan Lee Kuei-Chuan Lee Han-Chieh Lin Han-Chieh Lin Ming-Chih Hou Ming-Chih Hou |
| author_facet | An-Ming Yang An-Ming Yang Chien-Yu Lin Chien-Yu Lin Shih-Hao Liu Guan-Da Syu Guan-Da Syu Hao-Jhe Sun Kuei-Chuan Lee Kuei-Chuan Lee Han-Chieh Lin Han-Chieh Lin Ming-Chih Hou Ming-Chih Hou |
| author_sort | An-Ming Yang |
| collection | DOAJ |
| description | Non-alcoholic steatohepatitis (NASH) is affecting people worldwide. Changes in the intestinal microbiome are crucial to NASH. A previous study showed that eradicating intestinal fungi ameliorates NASH; however, the role of intestinal fungi in the development of NASH remains unclear. Saccharomyces boulardii (SB), a dietary supplement yeast, has been reported to restore the integrity of the intestine. Here, we tested the effect of SB in the treatment of NASH. For this study, we fed eight-week-old C57/BL6 male mice either a methionine-choline deficient (MCD) diet or a normal chow diet (NCD) for eight weeks. Half of the MCD diet-fed mice were gavaged with SB (5 mg/day) once daily. The remainder of the NCD–fed mice were gavaged with normal saline as a control. The MCD diet-fed mice on SB supplement showed better liver function, less hepatic steatosis, and decreased inflammation. Both hepatic inflammatory gene expression and fibrogenic gene expression were suppressed in mice with SB gavage. Intestinal damage caused by the MCD diet was tampered with, intestine inflammation decreased, and gut permeability improved in mice that had been given the SB supplement. Deep sequencing of the fecal microbiome showed a potentially increased beneficial gut microbiota and increased microbiota diversity in the SB-supplemented mice. The SB supplement maintains gut integrity, increases microbial diversity, and increases the number of potentially beneficial gut microbiota. Thus, the SB supplement attenuates gut leakage and exerts a protective effect against NASH. Our results provide new insight into the prevention of NASH. |
| first_indexed | 2024-12-12T15:47:59Z |
| format | Article |
| id | doaj.art-bc0bf620bc964a74a98b7eaa94340314 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T15:47:59Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-bc0bf620bc964a74a98b7eaa943403142022-12-22T00:19:42ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-06-011310.3389/fmicb.2022.887728887728Saccharomyces Boulardii Ameliorates Non-alcoholic Steatohepatitis in Mice Induced by a Methionine-Choline-Deficient Diet Through Gut-Liver AxisAn-Ming Yang0An-Ming Yang1Chien-Yu Lin2Chien-Yu Lin3Shih-Hao Liu4Guan-Da Syu5Guan-Da Syu6Hao-Jhe Sun7Kuei-Chuan Lee8Kuei-Chuan Lee9Han-Chieh Lin10Han-Chieh Lin11Ming-Chih Hou12Ming-Chih Hou13Department of Internal Medicine, En Chu Kong Hospital, New Taipei City, TaiwanDepartment of Nursing, Yuanpei University of Medical Technology, Hsinchu, TaiwanDepartment of Internal Medicine, En Chu Kong Hospital, New Taipei City, TaiwanSchool of Medicine, Fu Jen Catholic University, New Taipei City, TaiwanDivision of Pathology, En Chu Kong Hospital, New Taipei City, TaiwanDepartment of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan, TaiwanInternational Center for Wound Repair and Regeneration, National Cheng Kung University, Tainan, TaiwanDepartment of Medicine, National Yang Ming Chiao Tung University School of Medicine, Taipei City, TaiwanDepartment of Medicine, National Yang Ming Chiao Tung University School of Medicine, Taipei City, TaiwanDivision of Gastroenterology and Hepatology, Department of Medicine, Taipei Veterans General Hospital, Taipei City, TaiwanDepartment of Medicine, National Yang Ming Chiao Tung University School of Medicine, Taipei City, TaiwanDivision of Gastroenterology and Hepatology, Department of Medicine, Taipei Veterans General Hospital, Taipei City, TaiwanDepartment of Medicine, National Yang Ming Chiao Tung University School of Medicine, Taipei City, TaiwanDivision of Gastroenterology and Hepatology, Department of Medicine, Taipei Veterans General Hospital, Taipei City, TaiwanNon-alcoholic steatohepatitis (NASH) is affecting people worldwide. Changes in the intestinal microbiome are crucial to NASH. A previous study showed that eradicating intestinal fungi ameliorates NASH; however, the role of intestinal fungi in the development of NASH remains unclear. Saccharomyces boulardii (SB), a dietary supplement yeast, has been reported to restore the integrity of the intestine. Here, we tested the effect of SB in the treatment of NASH. For this study, we fed eight-week-old C57/BL6 male mice either a methionine-choline deficient (MCD) diet or a normal chow diet (NCD) for eight weeks. Half of the MCD diet-fed mice were gavaged with SB (5 mg/day) once daily. The remainder of the NCD–fed mice were gavaged with normal saline as a control. The MCD diet-fed mice on SB supplement showed better liver function, less hepatic steatosis, and decreased inflammation. Both hepatic inflammatory gene expression and fibrogenic gene expression were suppressed in mice with SB gavage. Intestinal damage caused by the MCD diet was tampered with, intestine inflammation decreased, and gut permeability improved in mice that had been given the SB supplement. Deep sequencing of the fecal microbiome showed a potentially increased beneficial gut microbiota and increased microbiota diversity in the SB-supplemented mice. The SB supplement maintains gut integrity, increases microbial diversity, and increases the number of potentially beneficial gut microbiota. Thus, the SB supplement attenuates gut leakage and exerts a protective effect against NASH. Our results provide new insight into the prevention of NASH.https://www.frontiersin.org/articles/10.3389/fmicb.2022.887728/fullnon-alcoholic steatohepatitisintestinal commensal fungiSaccharomyces boulardiimethionine-choline deficiency dietgut-liver axis |
| spellingShingle | An-Ming Yang An-Ming Yang Chien-Yu Lin Chien-Yu Lin Shih-Hao Liu Guan-Da Syu Guan-Da Syu Hao-Jhe Sun Kuei-Chuan Lee Kuei-Chuan Lee Han-Chieh Lin Han-Chieh Lin Ming-Chih Hou Ming-Chih Hou Saccharomyces Boulardii Ameliorates Non-alcoholic Steatohepatitis in Mice Induced by a Methionine-Choline-Deficient Diet Through Gut-Liver Axis Frontiers in Microbiology non-alcoholic steatohepatitis intestinal commensal fungi Saccharomyces boulardii methionine-choline deficiency diet gut-liver axis |
| title | Saccharomyces Boulardii Ameliorates Non-alcoholic Steatohepatitis in Mice Induced by a Methionine-Choline-Deficient Diet Through Gut-Liver Axis |
| title_full | Saccharomyces Boulardii Ameliorates Non-alcoholic Steatohepatitis in Mice Induced by a Methionine-Choline-Deficient Diet Through Gut-Liver Axis |
| title_fullStr | Saccharomyces Boulardii Ameliorates Non-alcoholic Steatohepatitis in Mice Induced by a Methionine-Choline-Deficient Diet Through Gut-Liver Axis |
| title_full_unstemmed | Saccharomyces Boulardii Ameliorates Non-alcoholic Steatohepatitis in Mice Induced by a Methionine-Choline-Deficient Diet Through Gut-Liver Axis |
| title_short | Saccharomyces Boulardii Ameliorates Non-alcoholic Steatohepatitis in Mice Induced by a Methionine-Choline-Deficient Diet Through Gut-Liver Axis |
| title_sort | saccharomyces boulardii ameliorates non alcoholic steatohepatitis in mice induced by a methionine choline deficient diet through gut liver axis |
| topic | non-alcoholic steatohepatitis intestinal commensal fungi Saccharomyces boulardii methionine-choline deficiency diet gut-liver axis |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.887728/full |
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