Molecular typing of clinical and environmental Aspergillus fumigatus isolates from Iran using microsatellites
Background and Purpose: Because of the growing incidence of Aspergillus infection,typing methods of Aspergillus species are increasingly being used. Accordingly,studying the spread and population dynamics of strains isolating from clinical and environment, from a single host to large scale ecosystem...
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| Format: | Article |
| Language: | English |
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Mazandaran University of Medical Sciences
2021-03-01
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| Series: | Current Medical Mycology |
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| Online Access: | http://cmm.mazums.ac.ir/article_129167_2704bae1e5607d66005c44fba34f7e94.pdf |
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| author | Hamid Badali Tahereh Shokohi Sadegh Khodavaysi Maryam Moazeni Masoumeh Farhadi Mojtaba Nabili |
| author_facet | Hamid Badali Tahereh Shokohi Sadegh Khodavaysi Maryam Moazeni Masoumeh Farhadi Mojtaba Nabili |
| author_sort | Hamid Badali |
| collection | DOAJ |
| description | Background and Purpose: Because of the growing incidence of Aspergillus infection,typing methods of Aspergillus species are increasingly being used. Accordingly,studying the spread and population dynamics of strains isolating from clinical and environment, from a single host to large scale ecosystems is definitely needed. In the current study, we carried out a genetic analysis of nine microsatellite loci in isolates from different regions of Iran to compare and explore the genetic diversity between environmental and clinical A. fumigatus strains. Materials and Methods: Sixty-six clinical (n=43) and environmental (n= 23) isolates of A. fumigatus, have collected from six cities of Iran. All A. fumigatus isolates identified based on macroscopic and microscopic characters, the ability to grow at above 45°C, and confirmed using DNA sequencing of the partial b-tubulin gene.Sixty-six A. fumigatus isolates were subjected by microsatellite typing using three separate multiplex PCRs with a panel of nine short tandem repeats (STR) to evaluate the genetic relatedness. Results: The STR typing of 66 A. fumigatus isolates revealed 38 distinct genotypes distributed among environmental and clinical isolates. We identified 12 clones including 40 different isolates representing 60% of all isolates tested, which each clone included 2–7 isolates. Conclusion: The STR typing is considered as a valuable tool with excellent discriminatory power to study the molecular epidemiology and genotypic diversity of A.fumigatus isolates. These findings show that the high genetic diversity observed of Iranian A. fumigatus isolates with those outside Iran and formed a separate cluster. |
| first_indexed | 2024-12-16T06:18:24Z |
| format | Article |
| id | doaj.art-bc0c20c824b94fb88e08edce1249474a |
| institution | Directory Open Access Journal |
| issn | 2423-3439 2423-3420 |
| language | English |
| last_indexed | 2024-12-16T06:18:24Z |
| publishDate | 2021-03-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Current Medical Mycology |
| spelling | doaj.art-bc0c20c824b94fb88e08edce1249474a2022-12-21T22:41:13ZengMazandaran University of Medical SciencesCurrent Medical Mycology2423-34392423-34202021-03-0171253010.18502/cmm.7.1.6180129167Molecular typing of clinical and environmental Aspergillus fumigatus isolates from Iran using microsatellitesHamid Badali0Tahereh Shokohi1Sadegh Khodavaysi2Maryam Moazeni3Masoumeh Farhadi4Mojtaba Nabili5Invasive Fungi Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, IranInvasive Fungi Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, IranDepartment of Medical Mycology and Parasitology, Tehran University of Medical Science, Tehran, IranInvasive Fungi Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, IranDepartment of Medical Laboratory Sciences, Faculty of Medicine, Sari Branch, Islamic Azad University, Sari, IranDepartment of Medical Laboratory Sciences, Faculty of Medicine, Sari Branch, Islamic Azad University, Sari, IranBackground and Purpose: Because of the growing incidence of Aspergillus infection,typing methods of Aspergillus species are increasingly being used. Accordingly,studying the spread and population dynamics of strains isolating from clinical and environment, from a single host to large scale ecosystems is definitely needed. In the current study, we carried out a genetic analysis of nine microsatellite loci in isolates from different regions of Iran to compare and explore the genetic diversity between environmental and clinical A. fumigatus strains. Materials and Methods: Sixty-six clinical (n=43) and environmental (n= 23) isolates of A. fumigatus, have collected from six cities of Iran. All A. fumigatus isolates identified based on macroscopic and microscopic characters, the ability to grow at above 45°C, and confirmed using DNA sequencing of the partial b-tubulin gene.Sixty-six A. fumigatus isolates were subjected by microsatellite typing using three separate multiplex PCRs with a panel of nine short tandem repeats (STR) to evaluate the genetic relatedness. Results: The STR typing of 66 A. fumigatus isolates revealed 38 distinct genotypes distributed among environmental and clinical isolates. We identified 12 clones including 40 different isolates representing 60% of all isolates tested, which each clone included 2–7 isolates. Conclusion: The STR typing is considered as a valuable tool with excellent discriminatory power to study the molecular epidemiology and genotypic diversity of A.fumigatus isolates. These findings show that the high genetic diversity observed of Iranian A. fumigatus isolates with those outside Iran and formed a separate cluster.http://cmm.mazums.ac.ir/article_129167_2704bae1e5607d66005c44fba34f7e94.pdfaspergillus fumigatusiranmicrosatellitemolecular typing |
| spellingShingle | Hamid Badali Tahereh Shokohi Sadegh Khodavaysi Maryam Moazeni Masoumeh Farhadi Mojtaba Nabili Molecular typing of clinical and environmental Aspergillus fumigatus isolates from Iran using microsatellites Current Medical Mycology aspergillus fumigatus iran microsatellite molecular typing |
| title | Molecular typing of clinical and environmental Aspergillus fumigatus isolates from Iran using microsatellites |
| title_full | Molecular typing of clinical and environmental Aspergillus fumigatus isolates from Iran using microsatellites |
| title_fullStr | Molecular typing of clinical and environmental Aspergillus fumigatus isolates from Iran using microsatellites |
| title_full_unstemmed | Molecular typing of clinical and environmental Aspergillus fumigatus isolates from Iran using microsatellites |
| title_short | Molecular typing of clinical and environmental Aspergillus fumigatus isolates from Iran using microsatellites |
| title_sort | molecular typing of clinical and environmental aspergillus fumigatus isolates from iran using microsatellites |
| topic | aspergillus fumigatus iran microsatellite molecular typing |
| url | http://cmm.mazums.ac.ir/article_129167_2704bae1e5607d66005c44fba34f7e94.pdf |
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