Characterization and Separation of Platinum-Based Antineoplastic Drugs by Zwitterionic Hydrophilic Interaction Liquid Chromatography (HILIC)–Tandem Mass Spectrometry, and Its Application in Surface Wipe Sampling
Platinum-based antineoplastic drugs (PtADs) are among the most important and used families of chemotherapy drugs, which, even showing severe side effects and being hindered by drug resistance, are not likely to be replaced clinically any time soon. The growing interest in the occupational health com...
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| Язык: | English |
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MDPI AG
2021-05-01
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| Серии: | Separations |
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| Online-ссылка: | https://www.mdpi.com/2297-8739/8/5/69 |
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| author | Stefano Dugheri Nicola Mucci Enrico Mini Donato Squillaci Giorgio Marrubini Gianluca Bartolucci Elisabetta Bucaletti Giovanni Cappelli Lucia Trevisani Giulio Arcangeli |
| author_facet | Stefano Dugheri Nicola Mucci Enrico Mini Donato Squillaci Giorgio Marrubini Gianluca Bartolucci Elisabetta Bucaletti Giovanni Cappelli Lucia Trevisani Giulio Arcangeli |
| author_sort | Stefano Dugheri |
| collection | DOAJ |
| description | Platinum-based antineoplastic drugs (PtADs) are among the most important and used families of chemotherapy drugs, which, even showing severe side effects and being hindered by drug resistance, are not likely to be replaced clinically any time soon. The growing interest in the occupational health community in antineoplastic drug (AD) surface contamination requires the development of increasingly fast and easy high-throughput monitoring methods, even considering the lack of harmonized legally binding regulation criteria. Thus, a wipe sampling method together with zwitterionic hydrophilic interaction liquid chromatography (HILIC-Z)–tandem mass spectrometry (MS/MS) analysis was developed for the simultaneous evaluation of oxaliplatin, cisplatin, and carboplatin surface contaminations. A design of experiments approach was used to optimize the chromatographic conditions. Limits of quantification ranging from 2 to 5 ng/mL were obtained from interday and intraday repetitions for oxaliplatin and carboplatin, and between 170 and 240 ng/mL for cisplatin. The wipe desorption procedure is equivalent to other AD sampling methods, enabling a fast sample preparation, with an LC-MS/MS analysis time of less than 7 min. |
| first_indexed | 2024-03-10T11:14:31Z |
| format | Article |
| id | doaj.art-bc0c8bc4e7eb42e7ac341d3d48c23f2d |
| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-03-10T11:14:31Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Separations |
| spelling | doaj.art-bc0c8bc4e7eb42e7ac341d3d48c23f2d2023-11-21T20:31:15ZengMDPI AGSeparations2297-87392021-05-01856910.3390/separations8050069Characterization and Separation of Platinum-Based Antineoplastic Drugs by Zwitterionic Hydrophilic Interaction Liquid Chromatography (HILIC)–Tandem Mass Spectrometry, and Its Application in Surface Wipe SamplingStefano Dugheri0Nicola Mucci1Enrico Mini2Donato Squillaci3Giorgio Marrubini4Gianluca Bartolucci5Elisabetta Bucaletti6Giovanni Cappelli7Lucia Trevisani8Giulio Arcangeli9Industrial Hygiene and Toxicology Laboratory, Occupational Medicine Unit, Careggi University Hospital, 50134 Florence, ItalyDepartment of Experimental and Clinical Medicine, University of Florence, 50134 Florence, ItalyDepartment of Health Sciences, University of Florence, 50134 Florence, ItalyDepartment of Experimental and Clinical Medicine, University of Florence, 50134 Florence, ItalyDepartment of Drug Sciences, University of Pavia, Via Taramelli 12, 27100 Pavia, ItalyDepartment of Neurosciences, Psychology, Drug Research and Child Health, University of Florence, 50019 Sesto Fiorentino, ItalyDepartment of Experimental and Clinical Medicine, University of Florence, 50134 Florence, ItalyDepartment of Experimental and Clinical Medicine, University of Florence, 50134 Florence, ItalyDepartment of Experimental and Clinical Medicine, University of Florence, 50134 Florence, ItalyDepartment of Experimental and Clinical Medicine, University of Florence, 50134 Florence, ItalyPlatinum-based antineoplastic drugs (PtADs) are among the most important and used families of chemotherapy drugs, which, even showing severe side effects and being hindered by drug resistance, are not likely to be replaced clinically any time soon. The growing interest in the occupational health community in antineoplastic drug (AD) surface contamination requires the development of increasingly fast and easy high-throughput monitoring methods, even considering the lack of harmonized legally binding regulation criteria. Thus, a wipe sampling method together with zwitterionic hydrophilic interaction liquid chromatography (HILIC-Z)–tandem mass spectrometry (MS/MS) analysis was developed for the simultaneous evaluation of oxaliplatin, cisplatin, and carboplatin surface contaminations. A design of experiments approach was used to optimize the chromatographic conditions. Limits of quantification ranging from 2 to 5 ng/mL were obtained from interday and intraday repetitions for oxaliplatin and carboplatin, and between 170 and 240 ng/mL for cisplatin. The wipe desorption procedure is equivalent to other AD sampling methods, enabling a fast sample preparation, with an LC-MS/MS analysis time of less than 7 min.https://www.mdpi.com/2297-8739/8/5/69platinum-based antineoplastic drugscisplatincarboplatinoxaliplatinHILIC–Zliquid chromatography–mass spectrometry |
| spellingShingle | Stefano Dugheri Nicola Mucci Enrico Mini Donato Squillaci Giorgio Marrubini Gianluca Bartolucci Elisabetta Bucaletti Giovanni Cappelli Lucia Trevisani Giulio Arcangeli Characterization and Separation of Platinum-Based Antineoplastic Drugs by Zwitterionic Hydrophilic Interaction Liquid Chromatography (HILIC)–Tandem Mass Spectrometry, and Its Application in Surface Wipe Sampling Separations platinum-based antineoplastic drugs cisplatin carboplatin oxaliplatin HILIC–Z liquid chromatography–mass spectrometry |
| title | Characterization and Separation of Platinum-Based Antineoplastic Drugs by Zwitterionic Hydrophilic Interaction Liquid Chromatography (HILIC)–Tandem Mass Spectrometry, and Its Application in Surface Wipe Sampling |
| title_full | Characterization and Separation of Platinum-Based Antineoplastic Drugs by Zwitterionic Hydrophilic Interaction Liquid Chromatography (HILIC)–Tandem Mass Spectrometry, and Its Application in Surface Wipe Sampling |
| title_fullStr | Characterization and Separation of Platinum-Based Antineoplastic Drugs by Zwitterionic Hydrophilic Interaction Liquid Chromatography (HILIC)–Tandem Mass Spectrometry, and Its Application in Surface Wipe Sampling |
| title_full_unstemmed | Characterization and Separation of Platinum-Based Antineoplastic Drugs by Zwitterionic Hydrophilic Interaction Liquid Chromatography (HILIC)–Tandem Mass Spectrometry, and Its Application in Surface Wipe Sampling |
| title_short | Characterization and Separation of Platinum-Based Antineoplastic Drugs by Zwitterionic Hydrophilic Interaction Liquid Chromatography (HILIC)–Tandem Mass Spectrometry, and Its Application in Surface Wipe Sampling |
| title_sort | characterization and separation of platinum based antineoplastic drugs by zwitterionic hydrophilic interaction liquid chromatography hilic tandem mass spectrometry and its application in surface wipe sampling |
| topic | platinum-based antineoplastic drugs cisplatin carboplatin oxaliplatin HILIC–Z liquid chromatography–mass spectrometry |
| url | https://www.mdpi.com/2297-8739/8/5/69 |
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