Diagnostic accuracy of IgA anti-tissue transglutaminase antibody testing in celiac disease
Contemporary guidelines for the first-line diagnosis of celiac disease recommend determination of IgA anti-tissue transglutaminase antibodies or IgA antiendomysial antibodies, as well as total serum IgA antibodies. The aim of our study was to assess the validity and clinical significance of serologi...
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| Format: | Article |
| Language: | English |
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Society of Medical Biochemists of Serbia, Belgrade
2012-01-01
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| Series: | Journal of Medical Biochemistry |
| Subjects: | |
| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2012/1452-82581202100E.pdf |
| _version_ | 1818410064929619968 |
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| author | Eremić Nevena Đerić Mirjana Hadnađev Ljiljana |
| author_facet | Eremić Nevena Đerić Mirjana Hadnađev Ljiljana |
| author_sort | Eremić Nevena |
| collection | DOAJ |
| description | Contemporary guidelines for the first-line diagnosis of celiac disease recommend determination of IgA anti-tissue transglutaminase antibodies or IgA antiendomysial antibodies, as well as total serum IgA antibodies. The aim of our study was to assess the validity and clinical significance of serological testing for IgA anti-tissue transglutaminase antibodies in the diagnosis of celiac disease, and to investigate the presence of malabsorption symptoms in celiac patients. IgA anti-tissue transglutaminase antibody testing was performed in 50 subjects with clinically suspected celiac disease (21 men and 29 women). All subjects underwent endoscopy with small intestine biopsy. Celiac disease was confirmed by histopathological findings in four subjects, whereas the IgA anti-tissue transglutaminase test was positive in three subjects. The IgA anti-tissue transglutaminase test showed sensitivity of 75% and specificity of 100%. There were significant differences between men with biopsy-confirmed and excluded celiac disease in the erythrocyte parameters MCV (96.5±7.7 vs. 78.6 ±11.3; p<0.05), MCH (36.9±4.6 vs. 25.9±4.9; p<0.01), and MCHC (382.5 ±16.3 vs. 326.9±19.1; p<0.005), as well as in the levels of total protein (47.5 ±16.3 vs. 68.3 ± 7.6; p<0.01) and albumins (24.6±9.5 vs. 42.1 ± 6.9; p<0.01). In addition, HDL-cholesterol levels were significantly lower in men with biopsy-confirmed celiac disease (0.42.±0.12 vs. 0.90± 0.30; p<0.05). Our results show a high correlation between IgA anti-tissue transglutaminase testing and endoscopy with biopsy as the gold diagnostic standard. |
| first_indexed | 2024-12-14T10:09:35Z |
| format | Article |
| id | doaj.art-bc0ef2cc623145d48ed6acfd330e941e |
| institution | Directory Open Access Journal |
| issn | 1452-8258 1452-8266 |
| language | English |
| last_indexed | 2024-12-14T10:09:35Z |
| publishDate | 2012-01-01 |
| publisher | Society of Medical Biochemists of Serbia, Belgrade |
| record_format | Article |
| series | Journal of Medical Biochemistry |
| spelling | doaj.art-bc0ef2cc623145d48ed6acfd330e941e2022-12-21T23:07:02ZengSociety of Medical Biochemists of Serbia, BelgradeJournal of Medical Biochemistry1452-82581452-82662012-01-013121001061452-82581202100EDiagnostic accuracy of IgA anti-tissue transglutaminase antibody testing in celiac diseaseEremić Nevena0Đerić Mirjana1Hadnađev Ljiljana2Center for Laboratory Medicine, Clinical Center of Vojvodina, Novi SadCenter for Laboratory Medicine, Clinical Center of Vojvodina, Novi SadGastroenterology and Hepatology Clinic, Clinical Center of Vojvodina, Novi SadContemporary guidelines for the first-line diagnosis of celiac disease recommend determination of IgA anti-tissue transglutaminase antibodies or IgA antiendomysial antibodies, as well as total serum IgA antibodies. The aim of our study was to assess the validity and clinical significance of serological testing for IgA anti-tissue transglutaminase antibodies in the diagnosis of celiac disease, and to investigate the presence of malabsorption symptoms in celiac patients. IgA anti-tissue transglutaminase antibody testing was performed in 50 subjects with clinically suspected celiac disease (21 men and 29 women). All subjects underwent endoscopy with small intestine biopsy. Celiac disease was confirmed by histopathological findings in four subjects, whereas the IgA anti-tissue transglutaminase test was positive in three subjects. The IgA anti-tissue transglutaminase test showed sensitivity of 75% and specificity of 100%. There were significant differences between men with biopsy-confirmed and excluded celiac disease in the erythrocyte parameters MCV (96.5±7.7 vs. 78.6 ±11.3; p<0.05), MCH (36.9±4.6 vs. 25.9±4.9; p<0.01), and MCHC (382.5 ±16.3 vs. 326.9±19.1; p<0.005), as well as in the levels of total protein (47.5 ±16.3 vs. 68.3 ± 7.6; p<0.01) and albumins (24.6±9.5 vs. 42.1 ± 6.9; p<0.01). In addition, HDL-cholesterol levels were significantly lower in men with biopsy-confirmed celiac disease (0.42.±0.12 vs. 0.90± 0.30; p<0.05). Our results show a high correlation between IgA anti-tissue transglutaminase testing and endoscopy with biopsy as the gold diagnostic standard.https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2012/1452-82581202100E.pdfceliac diseasetissue transglutaminaseantibodiesiga |
| spellingShingle | Eremić Nevena Đerić Mirjana Hadnađev Ljiljana Diagnostic accuracy of IgA anti-tissue transglutaminase antibody testing in celiac disease Journal of Medical Biochemistry celiac disease tissue transglutaminase antibodies iga |
| title | Diagnostic accuracy of IgA anti-tissue transglutaminase antibody testing in celiac disease |
| title_full | Diagnostic accuracy of IgA anti-tissue transglutaminase antibody testing in celiac disease |
| title_fullStr | Diagnostic accuracy of IgA anti-tissue transglutaminase antibody testing in celiac disease |
| title_full_unstemmed | Diagnostic accuracy of IgA anti-tissue transglutaminase antibody testing in celiac disease |
| title_short | Diagnostic accuracy of IgA anti-tissue transglutaminase antibody testing in celiac disease |
| title_sort | diagnostic accuracy of iga anti tissue transglutaminase antibody testing in celiac disease |
| topic | celiac disease tissue transglutaminase antibodies iga |
| url | https://scindeks-clanci.ceon.rs/data/pdf/1452-8258/2012/1452-82581202100E.pdf |
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