Synergistic mechanism of Bacillus subtilis Czk1 combined with propiconazole and tebuconazole mixtures against Pyrrhoderma noxium
Abstract Background Brown root rot disease is one of the devastating diseases in the rubber production process. It is not easy to be detected in the early stage of the disease. Our early research revealed that the rubber tree brown root rot fungus Pyrrhoderma noxium Pn006 was effectively inhibited b...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-10-01
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| Series: | Chemical and Biological Technologies in Agriculture |
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| Online Access: | https://doi.org/10.1186/s40538-023-00487-4 |
| _version_ | 1797453630660411392 |
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| author | Zi-wei Gu Jian-hang Yin He Wu Yan-qiong Liang Wei-huai Wu Ying Lu Rui Li Shi-bei Tan Chun-ping He Ke-xian Yi |
| author_facet | Zi-wei Gu Jian-hang Yin He Wu Yan-qiong Liang Wei-huai Wu Ying Lu Rui Li Shi-bei Tan Chun-ping He Ke-xian Yi |
| author_sort | Zi-wei Gu |
| collection | DOAJ |
| description | Abstract Background Brown root rot disease is one of the devastating diseases in the rubber production process. It is not easy to be detected in the early stage of the disease. Our early research revealed that the rubber tree brown root rot fungus Pyrrhoderma noxium Pn006 was effectively inhibited by Bacillus subtilis Czk1 and 25% propiconazole–tebuconazole, and that the two agents might work in synergy. Therefore, in this investigation, we used non-targeted metabolomic method to evaluate the synergistic mechanism of B. subtilis Czk1 and 25% propiconazole–tebuconazole on Pyrrhoderma noxium Pn006. Results Metabolomics analysis identified 708 unique metabolic markers, including mainly lipids and lipid-like molecules, organic acids, alcohols, ketones, alkaloids and their derivatives, nucleotides and their analogues, benzene ring compounds and amino acids and their derivatives. Further screening identified 105 key metabolic markers that could be potential biomarkers to reveal the mechanism of biocontrol bacteria and chemical fungicides combination synergy. Three key metabolic pathways were found in pathway enrichment, including linolenic acid metabolism, benzoic acid degradation, and valine, leucine and isoleucine degradation. Conclusions The mechanism might be related to the influence of the energy supply and cell functional integrity of pathogenic fungal cells. Critical relevance statement A metabolomic method was utilized to investigate the synergistic effects of B. subtilis Czk1 and 25% propiconazole–tebuconazole use on Pyrrhoderma noxium Pn006. Graphical Abstract |
| first_indexed | 2024-03-09T15:25:36Z |
| format | Article |
| id | doaj.art-bc0f3a9749ab428e859e0a83e5ac24f0 |
| institution | Directory Open Access Journal |
| issn | 2196-5641 |
| language | English |
| last_indexed | 2024-03-09T15:25:36Z |
| publishDate | 2023-10-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Chemical and Biological Technologies in Agriculture |
| spelling | doaj.art-bc0f3a9749ab428e859e0a83e5ac24f02023-11-26T12:33:17ZengSpringerOpenChemical and Biological Technologies in Agriculture2196-56412023-10-0110111410.1186/s40538-023-00487-4Synergistic mechanism of Bacillus subtilis Czk1 combined with propiconazole and tebuconazole mixtures against Pyrrhoderma noxiumZi-wei Gu0Jian-hang Yin1He Wu2Yan-qiong Liang3Wei-huai Wu4Ying Lu5Rui Li6Shi-bei Tan7Chun-ping He8Ke-xian Yi9School of Tropical Agriculture and Forestry, Hainan UniversityEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Science, Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural AffairsCollege of Agronomy and Biotechnology, Yunnan Agricultural UniversityEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Science, Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural AffairsEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Science, Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural AffairsEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Science, Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural AffairsEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Science, Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural AffairsEnvironment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Science, Key Laboratory of Integrated Pest Management on Tropical Crops, Ministry of Agriculture and Rural AffairsSchool of Tropical Agriculture and Forestry, Hainan UniversitySchool of Tropical Agriculture and Forestry, Hainan UniversityAbstract Background Brown root rot disease is one of the devastating diseases in the rubber production process. It is not easy to be detected in the early stage of the disease. Our early research revealed that the rubber tree brown root rot fungus Pyrrhoderma noxium Pn006 was effectively inhibited by Bacillus subtilis Czk1 and 25% propiconazole–tebuconazole, and that the two agents might work in synergy. Therefore, in this investigation, we used non-targeted metabolomic method to evaluate the synergistic mechanism of B. subtilis Czk1 and 25% propiconazole–tebuconazole on Pyrrhoderma noxium Pn006. Results Metabolomics analysis identified 708 unique metabolic markers, including mainly lipids and lipid-like molecules, organic acids, alcohols, ketones, alkaloids and their derivatives, nucleotides and their analogues, benzene ring compounds and amino acids and their derivatives. Further screening identified 105 key metabolic markers that could be potential biomarkers to reveal the mechanism of biocontrol bacteria and chemical fungicides combination synergy. Three key metabolic pathways were found in pathway enrichment, including linolenic acid metabolism, benzoic acid degradation, and valine, leucine and isoleucine degradation. Conclusions The mechanism might be related to the influence of the energy supply and cell functional integrity of pathogenic fungal cells. Critical relevance statement A metabolomic method was utilized to investigate the synergistic effects of B. subtilis Czk1 and 25% propiconazole–tebuconazole use on Pyrrhoderma noxium Pn006. Graphical Abstracthttps://doi.org/10.1186/s40538-023-00487-4Rubber tree root rot diseaseBacillus subtilis Czk125% propiconazole–tebuconazolePyrrhoderma noxiumCell metabolomics |
| spellingShingle | Zi-wei Gu Jian-hang Yin He Wu Yan-qiong Liang Wei-huai Wu Ying Lu Rui Li Shi-bei Tan Chun-ping He Ke-xian Yi Synergistic mechanism of Bacillus subtilis Czk1 combined with propiconazole and tebuconazole mixtures against Pyrrhoderma noxium Chemical and Biological Technologies in Agriculture Rubber tree root rot disease Bacillus subtilis Czk1 25% propiconazole–tebuconazole Pyrrhoderma noxium Cell metabolomics |
| title | Synergistic mechanism of Bacillus subtilis Czk1 combined with propiconazole and tebuconazole mixtures against Pyrrhoderma noxium |
| title_full | Synergistic mechanism of Bacillus subtilis Czk1 combined with propiconazole and tebuconazole mixtures against Pyrrhoderma noxium |
| title_fullStr | Synergistic mechanism of Bacillus subtilis Czk1 combined with propiconazole and tebuconazole mixtures against Pyrrhoderma noxium |
| title_full_unstemmed | Synergistic mechanism of Bacillus subtilis Czk1 combined with propiconazole and tebuconazole mixtures against Pyrrhoderma noxium |
| title_short | Synergistic mechanism of Bacillus subtilis Czk1 combined with propiconazole and tebuconazole mixtures against Pyrrhoderma noxium |
| title_sort | synergistic mechanism of bacillus subtilis czk1 combined with propiconazole and tebuconazole mixtures against pyrrhoderma noxium |
| topic | Rubber tree root rot disease Bacillus subtilis Czk1 25% propiconazole–tebuconazole Pyrrhoderma noxium Cell metabolomics |
| url | https://doi.org/10.1186/s40538-023-00487-4 |
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