Lactational performance of dairy cows in response to supplementing N-acetyl-l-methionine as source of rumen-protected methionine
ABSTRACT: The objective of this experiment was to evaluate the effects of supplementing a rumen-protected source of Met, N-acetyl-l-methionine (NALM), on lactational performance and nitrogen metabolism in early- to mid-lactation dairy cows. Sixty multiparous Holstein dairy cows in early lactation (2...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-03-01
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| Series: | Journal of Dairy Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022030221010778 |
| _version_ | 1828827595067621376 |
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| author | F.X. Amaro D. Kim R. Restelatto P. Carvalho K. Arriola E.J.C. Duvalsaint A.P. Cervantes Y. Jiang M.C.N. Agarussi V.P. Silva A.T. Adesogan L.F. Ferraretto C.R. Staples J.-S. Eun J.O. Moon D. Vyas |
| author_facet | F.X. Amaro D. Kim R. Restelatto P. Carvalho K. Arriola E.J.C. Duvalsaint A.P. Cervantes Y. Jiang M.C.N. Agarussi V.P. Silva A.T. Adesogan L.F. Ferraretto C.R. Staples J.-S. Eun J.O. Moon D. Vyas |
| author_sort | F.X. Amaro |
| collection | DOAJ |
| description | ABSTRACT: The objective of this experiment was to evaluate the effects of supplementing a rumen-protected source of Met, N-acetyl-l-methionine (NALM), on lactational performance and nitrogen metabolism in early- to mid-lactation dairy cows. Sixty multiparous Holstein dairy cows in early lactation (27 ± 4.3 d in milk, SD) were assigned to 4 treatments in a randomized complete block design. Cows were blocked by actual milk yield. Treatments were as follows: (1) no NALM (control); (2) 15 g/d of NALM (NALM15); (3) 30 g/d of NALM (NALM30); and (4) 45 g/d of NALM (NALM45). Diets were formulated using a Cornell Net Carbohydrate and Protein System (CNCPS) v.6.5 model software to meet or exceed nutritional requirements of lactating dairy cows producing 42 kg/d of milk and to undersupply metabolizable Met (control) or supply incremental amounts of NALM. The digestible Met (dMet) supply for control, NALM15, NALM30, and NALM45 were 54.7, 59.8, 64.7, and 72.2 g/d, respectively. The supply of dMet was 88, 94, 104, and 115% of dMet requirement for control, NALM15, NALM30, and NALM45, respectively. Milk yield data were collected, dry matter intake (DMI) was measured daily, and milk samples were collected twice per week for 22 wk. Blood, ruminal fluid, urine, and fecal samples were collected during the covariate period and during wk 4, 8, and 16. Data were analyzed using the GLIMMIX procedure of SAS (SAS Institute) using covariates in the model for all variables except body weight. Linear, quadratic, and cubic contrasts were also tested. Treatments did not affect DMI, milk yield, and milk component concentration and yield; however, feed efficiency expressed as milk yield per DMI and 3.5% fat-corrected milk per DMI were quadratically affected, with greater response observed for NALM15 and NALM30 compared with control. Acetate proportion linearly increased, whereas propionate proportion linearly decreased with NALM supplementation. Blood urea nitrogen linearly decreased with NALM supplementation. Total plasma essential AA concentrations were quadratically affected, as greater values were observed for control and NALM45 than other treatments. Plasma Met concentration was quadratically affected as lower levels were observed with NALM15, whereas Met concentrations increased with NALM45 compared with control. Nitrogen utilization efficiency and apparent total-tract nutrient digestibility were not affected by treatment. Supplementation of NALM at 15 or 30 g/head per day resulted in the greatest improvements in feed efficiency without affecting N metabolism of early- to mid-lactation dairy cows. |
| first_indexed | 2024-12-12T15:02:35Z |
| format | Article |
| id | doaj.art-bc0fc9d51884422689ef9efe043164d6 |
| institution | Directory Open Access Journal |
| issn | 0022-0302 |
| language | English |
| last_indexed | 2024-12-12T15:02:35Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Dairy Science |
| spelling | doaj.art-bc0fc9d51884422689ef9efe043164d62022-12-22T00:20:46ZengElsevierJournal of Dairy Science0022-03022022-03-01105323012314Lactational performance of dairy cows in response to supplementing N-acetyl-l-methionine as source of rumen-protected methionineF.X. Amaro0D. Kim1R. Restelatto2P. Carvalho3K. Arriola4E.J.C. Duvalsaint5A.P. Cervantes6Y. Jiang7M.C.N. Agarussi8V.P. Silva9A.T. Adesogan10L.F. Ferraretto11C.R. Staples12J.-S. Eun13J.O. Moon14D. Vyas15Department of Animal Sciences, University of Florida, Gainesville 32611Department of Animal Sciences, University of Florida, Gainesville 32611Department of Animal Sciences, University of Florida, Gainesville 32611; Universidade Federal do Parana, Curitiba, PR, 80060, BrazilDepartment of Animal Sciences, University of Florida, Gainesville 32611; Institute of Agriculture and Environmental Sciences, Federal University of Mato Grosso, Sinop, MT, 78557, BrazilDepartment of Animal Sciences, University of Florida, Gainesville 32611Department of Animal Sciences, University of Florida, Gainesville 32611Department of Animal Sciences, University of Florida, Gainesville 32611Department of Animal Sciences, University of Florida, Gainesville 32611Department of Animal Sciences, University of Florida, Gainesville 32611; Universidade Federal de Vicosa, Vicosa, MG, 36570, BrazilDepartment of Animal Sciences, University of Florida, Gainesville 32611; Universidade Federal de Vicosa, Vicosa, MG, 36570, BrazilDepartment of Animal Sciences, University of Florida, Gainesville 32611Department of Animal and Dairy Sciences, University of Wisconsin-Madison, Madison 53706Department of Animal Sciences, University of Florida, Gainesville 32611BIO Research Institute, CJ CheilJedang, Suwon, 04560, South KoreaBIO Research Institute, CJ CheilJedang, Suwon, 04560, South KoreaDepartment of Animal Sciences, University of Florida, Gainesville 32611; Corresponding authorABSTRACT: The objective of this experiment was to evaluate the effects of supplementing a rumen-protected source of Met, N-acetyl-l-methionine (NALM), on lactational performance and nitrogen metabolism in early- to mid-lactation dairy cows. Sixty multiparous Holstein dairy cows in early lactation (27 ± 4.3 d in milk, SD) were assigned to 4 treatments in a randomized complete block design. Cows were blocked by actual milk yield. Treatments were as follows: (1) no NALM (control); (2) 15 g/d of NALM (NALM15); (3) 30 g/d of NALM (NALM30); and (4) 45 g/d of NALM (NALM45). Diets were formulated using a Cornell Net Carbohydrate and Protein System (CNCPS) v.6.5 model software to meet or exceed nutritional requirements of lactating dairy cows producing 42 kg/d of milk and to undersupply metabolizable Met (control) or supply incremental amounts of NALM. The digestible Met (dMet) supply for control, NALM15, NALM30, and NALM45 were 54.7, 59.8, 64.7, and 72.2 g/d, respectively. The supply of dMet was 88, 94, 104, and 115% of dMet requirement for control, NALM15, NALM30, and NALM45, respectively. Milk yield data were collected, dry matter intake (DMI) was measured daily, and milk samples were collected twice per week for 22 wk. Blood, ruminal fluid, urine, and fecal samples were collected during the covariate period and during wk 4, 8, and 16. Data were analyzed using the GLIMMIX procedure of SAS (SAS Institute) using covariates in the model for all variables except body weight. Linear, quadratic, and cubic contrasts were also tested. Treatments did not affect DMI, milk yield, and milk component concentration and yield; however, feed efficiency expressed as milk yield per DMI and 3.5% fat-corrected milk per DMI were quadratically affected, with greater response observed for NALM15 and NALM30 compared with control. Acetate proportion linearly increased, whereas propionate proportion linearly decreased with NALM supplementation. Blood urea nitrogen linearly decreased with NALM supplementation. Total plasma essential AA concentrations were quadratically affected, as greater values were observed for control and NALM45 than other treatments. Plasma Met concentration was quadratically affected as lower levels were observed with NALM15, whereas Met concentrations increased with NALM45 compared with control. Nitrogen utilization efficiency and apparent total-tract nutrient digestibility were not affected by treatment. Supplementation of NALM at 15 or 30 g/head per day resulted in the greatest improvements in feed efficiency without affecting N metabolism of early- to mid-lactation dairy cows.http://www.sciencedirect.com/science/article/pii/S0022030221010778amino acid profilefeed efficiencymetabolizable methionineN-acetyl-l-methionine |
| spellingShingle | F.X. Amaro D. Kim R. Restelatto P. Carvalho K. Arriola E.J.C. Duvalsaint A.P. Cervantes Y. Jiang M.C.N. Agarussi V.P. Silva A.T. Adesogan L.F. Ferraretto C.R. Staples J.-S. Eun J.O. Moon D. Vyas Lactational performance of dairy cows in response to supplementing N-acetyl-l-methionine as source of rumen-protected methionine Journal of Dairy Science amino acid profile feed efficiency metabolizable methionine N-acetyl-l-methionine |
| title | Lactational performance of dairy cows in response to supplementing N-acetyl-l-methionine as source of rumen-protected methionine |
| title_full | Lactational performance of dairy cows in response to supplementing N-acetyl-l-methionine as source of rumen-protected methionine |
| title_fullStr | Lactational performance of dairy cows in response to supplementing N-acetyl-l-methionine as source of rumen-protected methionine |
| title_full_unstemmed | Lactational performance of dairy cows in response to supplementing N-acetyl-l-methionine as source of rumen-protected methionine |
| title_short | Lactational performance of dairy cows in response to supplementing N-acetyl-l-methionine as source of rumen-protected methionine |
| title_sort | lactational performance of dairy cows in response to supplementing n acetyl l methionine as source of rumen protected methionine |
| topic | amino acid profile feed efficiency metabolizable methionine N-acetyl-l-methionine |
| url | http://www.sciencedirect.com/science/article/pii/S0022030221010778 |
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