Unraveling the Secrets of Colistin Resistance with Label-Free Raman Spectroscopy
The rise in number of infections from multidrug-resistant (MDR) Gram-negative microbes has led to an increase in the use of a variety of ‘polymyxins’ such as colistin. Even though colistin is known to cause minor nephro- and neuro-toxicity, it is still considered as last resort antibiotic for treati...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/9/749 |
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| author | Dimple Saikia Priyanka Jadhav Arti R. Hole Chilakapati Murali Krishna Surya P. Singh |
| author_facet | Dimple Saikia Priyanka Jadhav Arti R. Hole Chilakapati Murali Krishna Surya P. Singh |
| author_sort | Dimple Saikia |
| collection | DOAJ |
| description | The rise in number of infections from multidrug-resistant (MDR) Gram-negative microbes has led to an increase in the use of a variety of ‘polymyxins’ such as colistin. Even though colistin is known to cause minor nephro- and neuro-toxicity, it is still considered as last resort antibiotic for treating MDR infections. In this study, we have applied Raman spectroscopy to understand the differences among colistin sensitive and resistant bacterial strains at community level. We have successfully generated colistin resistant clones and verified the presence of resistance-causing MCR-1 plasmid. A unique spectral profile associated with specific drug concentration has been obtained. Successful delineation between resistant and sensitive cells has also been achieved via principal component analysis. Overall findings support the prospective utility of Raman spectroscopy in identifying anti-microbial resistance. |
| first_indexed | 2024-03-10T00:35:42Z |
| format | Article |
| id | doaj.art-bc1513e2a17741418450ab3387ea3a33 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T00:35:42Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-bc1513e2a17741418450ab3387ea3a332023-11-23T15:18:31ZengMDPI AGBiosensors2079-63742022-09-0112974910.3390/bios12090749Unraveling the Secrets of Colistin Resistance with Label-Free Raman SpectroscopyDimple Saikia0Priyanka Jadhav1Arti R. Hole2Chilakapati Murali Krishna3Surya P. Singh4Department of Biosciences and Bioengineering, Indian Institute of Technology Dharwad, Dharwad 580011, IndiaTata Memorial Centre, Advanced Centre for Treatment Research and Education in Cancer, Navi Mumbai 410210, IndiaTata Memorial Centre, Advanced Centre for Treatment Research and Education in Cancer, Navi Mumbai 410210, IndiaTata Memorial Centre, Advanced Centre for Treatment Research and Education in Cancer, Navi Mumbai 410210, IndiaDepartment of Biosciences and Bioengineering, Indian Institute of Technology Dharwad, Dharwad 580011, IndiaThe rise in number of infections from multidrug-resistant (MDR) Gram-negative microbes has led to an increase in the use of a variety of ‘polymyxins’ such as colistin. Even though colistin is known to cause minor nephro- and neuro-toxicity, it is still considered as last resort antibiotic for treating MDR infections. In this study, we have applied Raman spectroscopy to understand the differences among colistin sensitive and resistant bacterial strains at community level. We have successfully generated colistin resistant clones and verified the presence of resistance-causing MCR-1 plasmid. A unique spectral profile associated with specific drug concentration has been obtained. Successful delineation between resistant and sensitive cells has also been achieved via principal component analysis. Overall findings support the prospective utility of Raman spectroscopy in identifying anti-microbial resistance.https://www.mdpi.com/2079-6374/12/9/749Raman spectroscopyantimicrobial resistancecolistinmicrobiologyprincipal component analysis |
| spellingShingle | Dimple Saikia Priyanka Jadhav Arti R. Hole Chilakapati Murali Krishna Surya P. Singh Unraveling the Secrets of Colistin Resistance with Label-Free Raman Spectroscopy Biosensors Raman spectroscopy antimicrobial resistance colistin microbiology principal component analysis |
| title | Unraveling the Secrets of Colistin Resistance with Label-Free Raman Spectroscopy |
| title_full | Unraveling the Secrets of Colistin Resistance with Label-Free Raman Spectroscopy |
| title_fullStr | Unraveling the Secrets of Colistin Resistance with Label-Free Raman Spectroscopy |
| title_full_unstemmed | Unraveling the Secrets of Colistin Resistance with Label-Free Raman Spectroscopy |
| title_short | Unraveling the Secrets of Colistin Resistance with Label-Free Raman Spectroscopy |
| title_sort | unraveling the secrets of colistin resistance with label free raman spectroscopy |
| topic | Raman spectroscopy antimicrobial resistance colistin microbiology principal component analysis |
| url | https://www.mdpi.com/2079-6374/12/9/749 |
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