Molecular identification and structural detection of anti-cancer compound from marine Streptomyces akiyoshiensis GRG 6 (KY457710) against MCF-7 breast cancer cells
The current study was concentrated to detection of anti-cancer compound from marine Streptomyces akiyoshiensis GRG 6 (KY457710) against MCF-7 breast cancer cells. The partial purification of TLC was moved three different active spots. Among the spots, the third spot of the extract was shown excellen...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-12-01
|
| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364720303153 |
| _version_ | 1818399877292359680 |
|---|---|
| author | Govindan Nadar Rajivgandhi Govindan Ramachandran Jia-Ling Li Lingzi Yin Natesan Manoharan Moorthy Rajesh Kannan Arockiam Antony Joseph Velanganni Naiyf S. Alharbi Shine Kadaikunnan Jamal M. Khaled Wen-Jun Li |
| author_facet | Govindan Nadar Rajivgandhi Govindan Ramachandran Jia-Ling Li Lingzi Yin Natesan Manoharan Moorthy Rajesh Kannan Arockiam Antony Joseph Velanganni Naiyf S. Alharbi Shine Kadaikunnan Jamal M. Khaled Wen-Jun Li |
| author_sort | Govindan Nadar Rajivgandhi |
| collection | DOAJ |
| description | The current study was concentrated to detection of anti-cancer compound from marine Streptomyces akiyoshiensis GRG 6 (KY457710) against MCF-7 breast cancer cells. The partial purification of TLC was moved three different active spots. Among the spots, the third spot of the extract was shown excellent anti-cancer properties against MCF-7 human breast cancer cells. The available anti-cancer compounds present in the partially purified extract was confirmed by GC–MS data interpretation. Three fractions of the compounds were purified by preparative HPLC and their respective spectrum was confirmed by analytical HPLC. Active anti-cancer fraction was separately purified by preparative HPLC and the cytotoxicity result was shown with 100 µg/mL concentration. The anti-cancer compound of pyrrolo [1,2-a]pyrazine-1,4-dione, hexahydro-3 was detected by LC-MS analysis after interpretation with GC–MS data. Further, the morphological damages and increased proliferation with more death cells of pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3 treated MCF-7 were observed by phase contrast and fluorescence microscope techniques. Finally, the nuclear condensation and increased necrotic cells of the pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3 treated MCF-7 cells was clearly identified by fluorescence microscope techniques using Hoechst 33,342 stain. Therefore, our result was prove, the pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3 rich Streptomyces akiyoshiensis GRG 6 (KY457710) as a potential anti-cancer agent against MCF-7 breast cancer cells. |
| first_indexed | 2024-12-14T07:27:39Z |
| format | Article |
| id | doaj.art-bc1680b66e6440b7b03c07ae3cd847e3 |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-12-14T07:27:39Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-bc1680b66e6440b7b03c07ae3cd847e32022-12-21T23:11:28ZengElsevierJournal of King Saud University: Science1018-36472020-12-0132834633469Molecular identification and structural detection of anti-cancer compound from marine Streptomyces akiyoshiensis GRG 6 (KY457710) against MCF-7 breast cancer cellsGovindan Nadar Rajivgandhi0Govindan Ramachandran1Jia-Ling Li2Lingzi Yin3Natesan Manoharan4Moorthy Rajesh Kannan5Arockiam Antony Joseph Velanganni6Naiyf S. Alharbi7Shine Kadaikunnan8Jamal M. Khaled9Wen-Jun Li10State Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China; Department of Marine Science, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India; State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, PR ChinaDepartment of Marine Science, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, IndiaState Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR ChinaState Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR ChinaDepartment of Marine Science, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, IndiaDepartment of Biochemistry, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, IndiaDepartment of Biochemistry, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, IndiaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi ArabiaState Key Laboratory of Biocontrol, Guangdong Provincial Key Laboratory of Plant Resources and Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai), School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China; State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, PR China; Corresponding author at: School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.The current study was concentrated to detection of anti-cancer compound from marine Streptomyces akiyoshiensis GRG 6 (KY457710) against MCF-7 breast cancer cells. The partial purification of TLC was moved three different active spots. Among the spots, the third spot of the extract was shown excellent anti-cancer properties against MCF-7 human breast cancer cells. The available anti-cancer compounds present in the partially purified extract was confirmed by GC–MS data interpretation. Three fractions of the compounds were purified by preparative HPLC and their respective spectrum was confirmed by analytical HPLC. Active anti-cancer fraction was separately purified by preparative HPLC and the cytotoxicity result was shown with 100 µg/mL concentration. The anti-cancer compound of pyrrolo [1,2-a]pyrazine-1,4-dione, hexahydro-3 was detected by LC-MS analysis after interpretation with GC–MS data. Further, the morphological damages and increased proliferation with more death cells of pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3 treated MCF-7 were observed by phase contrast and fluorescence microscope techniques. Finally, the nuclear condensation and increased necrotic cells of the pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3 treated MCF-7 cells was clearly identified by fluorescence microscope techniques using Hoechst 33,342 stain. Therefore, our result was prove, the pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3 rich Streptomyces akiyoshiensis GRG 6 (KY457710) as a potential anti-cancer agent against MCF-7 breast cancer cells.http://www.sciencedirect.com/science/article/pii/S1018364720303153ActinomycetesThin layer chromatographyPreparative HPLCCytotoxicity assayMorphological damagesNuclear damages |
| spellingShingle | Govindan Nadar Rajivgandhi Govindan Ramachandran Jia-Ling Li Lingzi Yin Natesan Manoharan Moorthy Rajesh Kannan Arockiam Antony Joseph Velanganni Naiyf S. Alharbi Shine Kadaikunnan Jamal M. Khaled Wen-Jun Li Molecular identification and structural detection of anti-cancer compound from marine Streptomyces akiyoshiensis GRG 6 (KY457710) against MCF-7 breast cancer cells Journal of King Saud University: Science Actinomycetes Thin layer chromatography Preparative HPLC Cytotoxicity assay Morphological damages Nuclear damages |
| title | Molecular identification and structural detection of anti-cancer compound from marine Streptomyces akiyoshiensis GRG 6 (KY457710) against MCF-7 breast cancer cells |
| title_full | Molecular identification and structural detection of anti-cancer compound from marine Streptomyces akiyoshiensis GRG 6 (KY457710) against MCF-7 breast cancer cells |
| title_fullStr | Molecular identification and structural detection of anti-cancer compound from marine Streptomyces akiyoshiensis GRG 6 (KY457710) against MCF-7 breast cancer cells |
| title_full_unstemmed | Molecular identification and structural detection of anti-cancer compound from marine Streptomyces akiyoshiensis GRG 6 (KY457710) against MCF-7 breast cancer cells |
| title_short | Molecular identification and structural detection of anti-cancer compound from marine Streptomyces akiyoshiensis GRG 6 (KY457710) against MCF-7 breast cancer cells |
| title_sort | molecular identification and structural detection of anti cancer compound from marine streptomyces akiyoshiensis grg 6 ky457710 against mcf 7 breast cancer cells |
| topic | Actinomycetes Thin layer chromatography Preparative HPLC Cytotoxicity assay Morphological damages Nuclear damages |
| url | http://www.sciencedirect.com/science/article/pii/S1018364720303153 |
| work_keys_str_mv | AT govindannadarrajivgandhi molecularidentificationandstructuraldetectionofanticancercompoundfrommarinestreptomycesakiyoshiensisgrg6ky457710againstmcf7breastcancercells AT govindanramachandran molecularidentificationandstructuraldetectionofanticancercompoundfrommarinestreptomycesakiyoshiensisgrg6ky457710againstmcf7breastcancercells AT jialingli molecularidentificationandstructuraldetectionofanticancercompoundfrommarinestreptomycesakiyoshiensisgrg6ky457710againstmcf7breastcancercells AT lingziyin molecularidentificationandstructuraldetectionofanticancercompoundfrommarinestreptomycesakiyoshiensisgrg6ky457710againstmcf7breastcancercells AT natesanmanoharan molecularidentificationandstructuraldetectionofanticancercompoundfrommarinestreptomycesakiyoshiensisgrg6ky457710againstmcf7breastcancercells AT moorthyrajeshkannan molecularidentificationandstructuraldetectionofanticancercompoundfrommarinestreptomycesakiyoshiensisgrg6ky457710againstmcf7breastcancercells AT arockiamantonyjosephvelanganni molecularidentificationandstructuraldetectionofanticancercompoundfrommarinestreptomycesakiyoshiensisgrg6ky457710againstmcf7breastcancercells AT naiyfsalharbi molecularidentificationandstructuraldetectionofanticancercompoundfrommarinestreptomycesakiyoshiensisgrg6ky457710againstmcf7breastcancercells AT shinekadaikunnan molecularidentificationandstructuraldetectionofanticancercompoundfrommarinestreptomycesakiyoshiensisgrg6ky457710againstmcf7breastcancercells AT jamalmkhaled molecularidentificationandstructuraldetectionofanticancercompoundfrommarinestreptomycesakiyoshiensisgrg6ky457710againstmcf7breastcancercells AT wenjunli molecularidentificationandstructuraldetectionofanticancercompoundfrommarinestreptomycesakiyoshiensisgrg6ky457710againstmcf7breastcancercells |