<i>CaSBP11</i> Participates in the Defense Response of Pepper to <i>Phytophthora capsici</i> through Regulating the Expression of Defense-Related Genes
Squamosa promoter binding protein (SBP)-box genes are plant-specific transcription factors involved in plant growth and development, morphogenesis and biotic and abiotic stress responses. However, these genes have been understudied in pepper, especially with respect to defense responses to <i>...
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| Format: | Article |
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MDPI AG
2020-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/23/9065 |
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| author | Huai-Xia Zhang Xiao-Hui Feng Jing-Hao Jin Abid Khan Wei-Li Guo Xiao-Hua Du Zhen-Hui Gong |
| author_facet | Huai-Xia Zhang Xiao-Hui Feng Jing-Hao Jin Abid Khan Wei-Li Guo Xiao-Hua Du Zhen-Hui Gong |
| author_sort | Huai-Xia Zhang |
| collection | DOAJ |
| description | Squamosa promoter binding protein (SBP)-box genes are plant-specific transcription factors involved in plant growth and development, morphogenesis and biotic and abiotic stress responses. However, these genes have been understudied in pepper, especially with respect to defense responses to <i>Phytophthora capsici</i> infection. <i>CaSBP11</i> is a SBP-box family gene in pepper that was identified in our previous research. Silencing <i>CaSBP11</i> enhanced the defense response of pepper plants to <i>Phytophthora capsici</i>. Without treatment, the expression of defense-related genes (<i>CaBPR1</i>, <i>CaPO1</i>, <i>CaSAR8.2</i> and <i>CaDEF1</i>) increased in <i>CaSBP11</i>-silenced plants. However, the expression levels of these genes were inhibited under transient <i>CaSBP11</i> expression. <i>CaSBP11</i> overexpression in transgenic <i>Nicotiana benthamiana</i> decreased defense responses, while in <i>Arabidopsis</i>, it induced or inhibited the expression of genes in the salicylic acid and jasmonic acid signaling pathways. <i>CaSBP11</i> overexpression in <i>sid2-2</i> mutants induced <i>AtNPR1</i>, <i>AtNPR3</i>, <i>AtNPR4</i>, <i>AtPAD4</i>, <i>AtEDS1</i>, <i>AtEDS5</i>, <i>AtMPK4</i> and <i>AtNDR1</i> expression, while <i>AtSARD1</i> and <i>AtTGA6</i> expression was inhibited. <i>CaSBP11</i> overexpression in <i>coi1-21</i> and <i>coi1-22</i> mutants, respectively, inhibited <i>AtPDF1.2</i> expression and induced <i>AtPR1</i> expression. These results indicate <i>CaSBP11</i> has a negative regulatory effect on defense responses to <i>Phytophthora capsici</i>. Moreover, it may participate in the defense response of pepper to <i>Phytophthora capsici</i> by regulating defense-related genes and the salicylic and jasmonic acid-mediated disease resistance signaling pathways. |
| first_indexed | 2024-03-10T14:28:28Z |
| format | Article |
| id | doaj.art-bc17bccd6cbe48119c549866d02ccabf |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T14:28:28Z |
| publishDate | 2020-11-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-bc17bccd6cbe48119c549866d02ccabf2023-11-20T22:47:24ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-11-012123906510.3390/ijms21239065<i>CaSBP11</i> Participates in the Defense Response of Pepper to <i>Phytophthora capsici</i> through Regulating the Expression of Defense-Related GenesHuai-Xia Zhang0Xiao-Hui Feng1Jing-Hao Jin2Abid Khan3Wei-Li Guo4Xiao-Hua Du5Zhen-Hui Gong6College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, ChinaCollege of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, ChinaCollege of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, ChinaDepartment of Horticulture, The University of Haripur, Haripur 22620, PakistanSchool of Horticulture Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, ChinaSchool of Horticulture Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, ChinaCollege of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, ChinaSquamosa promoter binding protein (SBP)-box genes are plant-specific transcription factors involved in plant growth and development, morphogenesis and biotic and abiotic stress responses. However, these genes have been understudied in pepper, especially with respect to defense responses to <i>Phytophthora capsici</i> infection. <i>CaSBP11</i> is a SBP-box family gene in pepper that was identified in our previous research. Silencing <i>CaSBP11</i> enhanced the defense response of pepper plants to <i>Phytophthora capsici</i>. Without treatment, the expression of defense-related genes (<i>CaBPR1</i>, <i>CaPO1</i>, <i>CaSAR8.2</i> and <i>CaDEF1</i>) increased in <i>CaSBP11</i>-silenced plants. However, the expression levels of these genes were inhibited under transient <i>CaSBP11</i> expression. <i>CaSBP11</i> overexpression in transgenic <i>Nicotiana benthamiana</i> decreased defense responses, while in <i>Arabidopsis</i>, it induced or inhibited the expression of genes in the salicylic acid and jasmonic acid signaling pathways. <i>CaSBP11</i> overexpression in <i>sid2-2</i> mutants induced <i>AtNPR1</i>, <i>AtNPR3</i>, <i>AtNPR4</i>, <i>AtPAD4</i>, <i>AtEDS1</i>, <i>AtEDS5</i>, <i>AtMPK4</i> and <i>AtNDR1</i> expression, while <i>AtSARD1</i> and <i>AtTGA6</i> expression was inhibited. <i>CaSBP11</i> overexpression in <i>coi1-21</i> and <i>coi1-22</i> mutants, respectively, inhibited <i>AtPDF1.2</i> expression and induced <i>AtPR1</i> expression. These results indicate <i>CaSBP11</i> has a negative regulatory effect on defense responses to <i>Phytophthora capsici</i>. Moreover, it may participate in the defense response of pepper to <i>Phytophthora capsici</i> by regulating defense-related genes and the salicylic and jasmonic acid-mediated disease resistance signaling pathways.https://www.mdpi.com/1422-0067/21/23/9065pepper<i>CaSBP11</i><i>Phytophthora capsici</i>defense-related genes<i>Nicotiana benthamiana</i> |
| spellingShingle | Huai-Xia Zhang Xiao-Hui Feng Jing-Hao Jin Abid Khan Wei-Li Guo Xiao-Hua Du Zhen-Hui Gong <i>CaSBP11</i> Participates in the Defense Response of Pepper to <i>Phytophthora capsici</i> through Regulating the Expression of Defense-Related Genes International Journal of Molecular Sciences pepper <i>CaSBP11</i> <i>Phytophthora capsici</i> defense-related genes <i>Nicotiana benthamiana</i> |
| title | <i>CaSBP11</i> Participates in the Defense Response of Pepper to <i>Phytophthora capsici</i> through Regulating the Expression of Defense-Related Genes |
| title_full | <i>CaSBP11</i> Participates in the Defense Response of Pepper to <i>Phytophthora capsici</i> through Regulating the Expression of Defense-Related Genes |
| title_fullStr | <i>CaSBP11</i> Participates in the Defense Response of Pepper to <i>Phytophthora capsici</i> through Regulating the Expression of Defense-Related Genes |
| title_full_unstemmed | <i>CaSBP11</i> Participates in the Defense Response of Pepper to <i>Phytophthora capsici</i> through Regulating the Expression of Defense-Related Genes |
| title_short | <i>CaSBP11</i> Participates in the Defense Response of Pepper to <i>Phytophthora capsici</i> through Regulating the Expression of Defense-Related Genes |
| title_sort | i casbp11 i participates in the defense response of pepper to i phytophthora capsici i through regulating the expression of defense related genes |
| topic | pepper <i>CaSBP11</i> <i>Phytophthora capsici</i> defense-related genes <i>Nicotiana benthamiana</i> |
| url | https://www.mdpi.com/1422-0067/21/23/9065 |
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