Alleviative Effect of Iodine Pretreatment on the Stress of <i>Saccharina japonica</i> (Phaeophyceae, Laminariales) Caused by Cadmium and Its Molecular Basis Revealed by Comparative Transcriptomic Analysis
Iodide is accumulated by the brown alga <i>Saccharina japonica</i> at a high concentration and has been proven to be an inorganic antioxidant that plays an important role in oxidative metabolism. Vanadium-dependent bromoperoxidases (vBPOs) and iodoperoxidases (vIPOs), which catalyze the...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/19/14825 |
| _version_ | 1797575771355611136 |
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| author | Xuemei Wang Tifeng Shan Shaojun Pang |
| author_facet | Xuemei Wang Tifeng Shan Shaojun Pang |
| author_sort | Xuemei Wang |
| collection | DOAJ |
| description | Iodide is accumulated by the brown alga <i>Saccharina japonica</i> at a high concentration and has been proven to be an inorganic antioxidant that plays an important role in oxidative metabolism. Vanadium-dependent bromoperoxidases (vBPOs) and iodoperoxidases (vIPOs), which catalyze the oxidation of iodide, are essential for iodine accumulation and metabolism. Heavy metal pollutant cadmium (Cd) from anthropogenic activities can cause damage to algae mainly by producing oxidative stress. Here, the effects of iodine pretreatment on the stress of <i>S. japonica</i> caused by cadmium were analyzed. The growth experiment showed that iodine pretreatment could reduce the damage of low concentration cadmium on <i>S. japonica</i> young thalli. At the transcriptomic level, gene ontology (GO) enrichment analysis confirmed that cadmium stress could cause a peroxidation reaction in <i>S. japonica</i>. However, the most significant GO term was “photosystem I” in the series with iodine pretreatment. Weighted gene co-expression network analysis (WGCNA) indicated that iodine pretreatment alleviated cadmium stress responses of <i>S. japonica</i> by affecting the photosynthesis process. Analysis of the differentially expressed genes (DEGs) showed that five enzymes from the vBPO family and 13 enzymes from the vIPO family might play crucial roles in this process. |
| first_indexed | 2024-03-10T21:43:04Z |
| format | Article |
| id | doaj.art-bc17c9c1914e4ed4a132df08d4d30580 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T21:43:04Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-bc17c9c1914e4ed4a132df08d4d305802023-11-19T14:31:13ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-10-0124191482510.3390/ijms241914825Alleviative Effect of Iodine Pretreatment on the Stress of <i>Saccharina japonica</i> (Phaeophyceae, Laminariales) Caused by Cadmium and Its Molecular Basis Revealed by Comparative Transcriptomic AnalysisXuemei Wang0Tifeng Shan1Shaojun Pang2Chinese Academy of Sciences and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaChinese Academy of Sciences and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaChinese Academy of Sciences and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaIodide is accumulated by the brown alga <i>Saccharina japonica</i> at a high concentration and has been proven to be an inorganic antioxidant that plays an important role in oxidative metabolism. Vanadium-dependent bromoperoxidases (vBPOs) and iodoperoxidases (vIPOs), which catalyze the oxidation of iodide, are essential for iodine accumulation and metabolism. Heavy metal pollutant cadmium (Cd) from anthropogenic activities can cause damage to algae mainly by producing oxidative stress. Here, the effects of iodine pretreatment on the stress of <i>S. japonica</i> caused by cadmium were analyzed. The growth experiment showed that iodine pretreatment could reduce the damage of low concentration cadmium on <i>S. japonica</i> young thalli. At the transcriptomic level, gene ontology (GO) enrichment analysis confirmed that cadmium stress could cause a peroxidation reaction in <i>S. japonica</i>. However, the most significant GO term was “photosystem I” in the series with iodine pretreatment. Weighted gene co-expression network analysis (WGCNA) indicated that iodine pretreatment alleviated cadmium stress responses of <i>S. japonica</i> by affecting the photosynthesis process. Analysis of the differentially expressed genes (DEGs) showed that five enzymes from the vBPO family and 13 enzymes from the vIPO family might play crucial roles in this process.https://www.mdpi.com/1422-0067/24/19/14825<i>Saccharina japonica</i>iodinetranscriptomecadmium stressvHPOs |
| spellingShingle | Xuemei Wang Tifeng Shan Shaojun Pang Alleviative Effect of Iodine Pretreatment on the Stress of <i>Saccharina japonica</i> (Phaeophyceae, Laminariales) Caused by Cadmium and Its Molecular Basis Revealed by Comparative Transcriptomic Analysis International Journal of Molecular Sciences <i>Saccharina japonica</i> iodine transcriptome cadmium stress vHPOs |
| title | Alleviative Effect of Iodine Pretreatment on the Stress of <i>Saccharina japonica</i> (Phaeophyceae, Laminariales) Caused by Cadmium and Its Molecular Basis Revealed by Comparative Transcriptomic Analysis |
| title_full | Alleviative Effect of Iodine Pretreatment on the Stress of <i>Saccharina japonica</i> (Phaeophyceae, Laminariales) Caused by Cadmium and Its Molecular Basis Revealed by Comparative Transcriptomic Analysis |
| title_fullStr | Alleviative Effect of Iodine Pretreatment on the Stress of <i>Saccharina japonica</i> (Phaeophyceae, Laminariales) Caused by Cadmium and Its Molecular Basis Revealed by Comparative Transcriptomic Analysis |
| title_full_unstemmed | Alleviative Effect of Iodine Pretreatment on the Stress of <i>Saccharina japonica</i> (Phaeophyceae, Laminariales) Caused by Cadmium and Its Molecular Basis Revealed by Comparative Transcriptomic Analysis |
| title_short | Alleviative Effect of Iodine Pretreatment on the Stress of <i>Saccharina japonica</i> (Phaeophyceae, Laminariales) Caused by Cadmium and Its Molecular Basis Revealed by Comparative Transcriptomic Analysis |
| title_sort | alleviative effect of iodine pretreatment on the stress of i saccharina japonica i phaeophyceae laminariales caused by cadmium and its molecular basis revealed by comparative transcriptomic analysis |
| topic | <i>Saccharina japonica</i> iodine transcriptome cadmium stress vHPOs |
| url | https://www.mdpi.com/1422-0067/24/19/14825 |
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