Preemptive analgesic effects of midazolam and diclofenac in rat model
The aim of the present study was to investigate the preemptive analgesic effects of intraperitoneally administrated midazolam and diclofenac, before acute and inflammatory induced pain in rat model. One hundred twenty-eight (n=8 in each group) male Sprague Dawley rats were included in the study. P...
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| Format: | Article |
| Language: | English |
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Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2011-05-01
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| Series: | Biomolecules & Biomedicine |
| Subjects: | |
| Online Access: | https://www.bjbms.org/ojs/index.php/bjbms/article/view/2593 |
| _version_ | 1797260907907121152 |
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| author | Antigona Hasani Marija Soljakova Muharrem Jakupi Serpil Ustalar-Ozgen |
| author_facet | Antigona Hasani Marija Soljakova Muharrem Jakupi Serpil Ustalar-Ozgen |
| author_sort | Antigona Hasani |
| collection | DOAJ |
| description | The aim of the present study was to investigate the preemptive analgesic effects of intraperitoneally administrated midazolam and diclofenac, before acute and inflammatory induced pain in rat model.
One hundred twenty-eight (n=8 in each group) male Sprague Dawley rats were included in the study. Paw movements in response to thermal stimulation or paw flinching in response to formalin injection were compared after midazolam (0.1, 1, 5 and 10 mg/kg) and diclofenac (10 mg/kg), intraperitoneal administration. Saline was used as a control.
Preemptive analgesic effect was significant in both tests when diclofenac and midazolam was administrated before the pain stimuli (p<0.01 and p<0.001). Intraperitoneal injection of midazolam in doses 5 and 10 mg/kg, increase the response time in hot plate test and decrease the number of flinches in formalin test (p<0.01 vs. p<0.001). ED50 of midazolam (with diclofenac) in hot plate test was 2.02 mg/kg (CI95% =-3.47-5.03 mg); and, 0.9 mg/kg (CI95% =-0.87-4.09 mg) in phase I and 0.7 mg/kg (CI95% = 0.48-6.63 mg) in phase II, in formalin test.
Intraperitoneally administered midazolam and diclofenac had preemptive analgesic effects on acute thermal, and inflammatory induced pain in rats.
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| first_indexed | 2024-04-24T23:32:47Z |
| format | Article |
| id | doaj.art-bc18347e6a7a460d9b33242b8c6e5b17 |
| institution | Directory Open Access Journal |
| issn | 2831-0896 2831-090X |
| language | English |
| last_indexed | 2024-04-24T23:32:47Z |
| publishDate | 2011-05-01 |
| publisher | Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
| record_format | Article |
| series | Biomolecules & Biomedicine |
| spelling | doaj.art-bc18347e6a7a460d9b33242b8c6e5b172024-03-15T14:33:35ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2011-05-0111210.17305/bjbms.2011.2593353Preemptive analgesic effects of midazolam and diclofenac in rat modelAntigona Hasani0Marija Soljakova1Muharrem Jakupi2Serpil Ustalar-Ozgen3Department of Anesthesiology and Reanimation, University Clinical Centre of KosovoDepartment of Anesthesiology and Reanimation, Faculty of Medicine “St. Cyril and Methodius”Institute of Clinical Pharmacology, Clinical Centre University of Prishtina, Faculty of MedicineDepartment of Anesthesiology and Reanimation, Acibadem UniversitesiThe aim of the present study was to investigate the preemptive analgesic effects of intraperitoneally administrated midazolam and diclofenac, before acute and inflammatory induced pain in rat model. One hundred twenty-eight (n=8 in each group) male Sprague Dawley rats were included in the study. Paw movements in response to thermal stimulation or paw flinching in response to formalin injection were compared after midazolam (0.1, 1, 5 and 10 mg/kg) and diclofenac (10 mg/kg), intraperitoneal administration. Saline was used as a control. Preemptive analgesic effect was significant in both tests when diclofenac and midazolam was administrated before the pain stimuli (p<0.01 and p<0.001). Intraperitoneal injection of midazolam in doses 5 and 10 mg/kg, increase the response time in hot plate test and decrease the number of flinches in formalin test (p<0.01 vs. p<0.001). ED50 of midazolam (with diclofenac) in hot plate test was 2.02 mg/kg (CI95% =-3.47-5.03 mg); and, 0.9 mg/kg (CI95% =-0.87-4.09 mg) in phase I and 0.7 mg/kg (CI95% = 0.48-6.63 mg) in phase II, in formalin test. Intraperitoneally administered midazolam and diclofenac had preemptive analgesic effects on acute thermal, and inflammatory induced pain in rats. https://www.bjbms.org/ojs/index.php/bjbms/article/view/2593midazolamdiclofenacpreemptive analgesiahot plate testformalin test |
| spellingShingle | Antigona Hasani Marija Soljakova Muharrem Jakupi Serpil Ustalar-Ozgen Preemptive analgesic effects of midazolam and diclofenac in rat model Biomolecules & Biomedicine midazolam diclofenac preemptive analgesia hot plate test formalin test |
| title | Preemptive analgesic effects of midazolam and diclofenac in rat model |
| title_full | Preemptive analgesic effects of midazolam and diclofenac in rat model |
| title_fullStr | Preemptive analgesic effects of midazolam and diclofenac in rat model |
| title_full_unstemmed | Preemptive analgesic effects of midazolam and diclofenac in rat model |
| title_short | Preemptive analgesic effects of midazolam and diclofenac in rat model |
| title_sort | preemptive analgesic effects of midazolam and diclofenac in rat model |
| topic | midazolam diclofenac preemptive analgesia hot plate test formalin test |
| url | https://www.bjbms.org/ojs/index.php/bjbms/article/view/2593 |
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