The <i>LEPR</i> Gene Is Associated with Reproductive Seasonality Traits in Rasa Aragonesa Sheep
The aim of this study was to characterize and identify causative polymorphisms in the <i>leptin receptor</i> (<i>LEPR</i>) gene responsible for the seasonal variation of reproductive traits in sheep. Three reproductive seasonality traits were studied: the total days of anoest...
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MDPI AG
2020-12-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/12/2448 |
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| author | Kenza Lakhssassi Malena Serrano Belén Lahoz María Pilar Sarto Laura Pilar Iguácel José Folch José Luis Alabart Jorge Hugo Calvo |
| author_facet | Kenza Lakhssassi Malena Serrano Belén Lahoz María Pilar Sarto Laura Pilar Iguácel José Folch José Luis Alabart Jorge Hugo Calvo |
| author_sort | Kenza Lakhssassi |
| collection | DOAJ |
| description | The aim of this study was to characterize and identify causative polymorphisms in the <i>leptin receptor</i> (<i>LEPR</i>) gene responsible for the seasonal variation of reproductive traits in sheep. Three reproductive seasonality traits were studied: the total days of anoestrous (TDA), the progesterone cycling months (P4CM) and the oestrous cycling months (OCM). In total, 18 SNPs were detected in 33 ewes with extreme values for TDA and OCM. Six SNPs were non-synonymous substitutions and two of them were predicted in silico as deleterious: rs596133197 and rs403578195. These polymorphisms were then validated in 239 ewes. The SNP rs403578195, located in exon 8 and leading to a change of alanine to glycine (Ala284Gly) in the extracellular domain of the protein, was associated with the OCM trait, being the G allele associated with a decrease of 12 percent of the OCM trait. Haplotype analyses also suggested the involvement of other non-synonymous SNP located in exon 20 (rs405459906). This SNP also produces an amino acid change (Lys1069Glu) in the intracellular domain of the protein and segregates independently of rs403578195. These results confirm for the first time the role of the <i>LEPR</i> gene in sheep reproductive seasonality. |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T13:54:32Z |
| publishDate | 2020-12-01 |
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| series | Animals |
| spelling | doaj.art-bc1a8a0422cf4219a7fc1d23e4ba06782023-11-21T01:50:05ZengMDPI AGAnimals2076-26152020-12-011012244810.3390/ani10122448The <i>LEPR</i> Gene Is Associated with Reproductive Seasonality Traits in Rasa Aragonesa SheepKenza Lakhssassi0Malena Serrano1Belén Lahoz2María Pilar Sarto3Laura Pilar Iguácel4José Folch5José Luis Alabart6Jorge Hugo Calvo7Centro de Investigación y Tecnología Agroalimentaria de Aragón, Instituto Agroalimentario de Aragón (IA2), CITA–Zaragoza University, 50059 Zaragoza, SpainDepartamento de Mejora Genética Animal INIA, 28040 Madrid, SpainCentro de Investigación y Tecnología Agroalimentaria de Aragón, Instituto Agroalimentario de Aragón (IA2), CITA–Zaragoza University, 50059 Zaragoza, SpainCentro de Investigación y Tecnología Agroalimentaria de Aragón, Instituto Agroalimentario de Aragón (IA2), CITA–Zaragoza University, 50059 Zaragoza, SpainCentro de Investigación y Tecnología Agroalimentaria de Aragón, Instituto Agroalimentario de Aragón (IA2), CITA–Zaragoza University, 50059 Zaragoza, SpainCentro de Investigación y Tecnología Agroalimentaria de Aragón, Instituto Agroalimentario de Aragón (IA2), CITA–Zaragoza University, 50059 Zaragoza, SpainCentro de Investigación y Tecnología Agroalimentaria de Aragón, Instituto Agroalimentario de Aragón (IA2), CITA–Zaragoza University, 50059 Zaragoza, SpainCentro de Investigación y Tecnología Agroalimentaria de Aragón, Instituto Agroalimentario de Aragón (IA2), CITA–Zaragoza University, 50059 Zaragoza, SpainThe aim of this study was to characterize and identify causative polymorphisms in the <i>leptin receptor</i> (<i>LEPR</i>) gene responsible for the seasonal variation of reproductive traits in sheep. Three reproductive seasonality traits were studied: the total days of anoestrous (TDA), the progesterone cycling months (P4CM) and the oestrous cycling months (OCM). In total, 18 SNPs were detected in 33 ewes with extreme values for TDA and OCM. Six SNPs were non-synonymous substitutions and two of them were predicted in silico as deleterious: rs596133197 and rs403578195. These polymorphisms were then validated in 239 ewes. The SNP rs403578195, located in exon 8 and leading to a change of alanine to glycine (Ala284Gly) in the extracellular domain of the protein, was associated with the OCM trait, being the G allele associated with a decrease of 12 percent of the OCM trait. Haplotype analyses also suggested the involvement of other non-synonymous SNP located in exon 20 (rs405459906). This SNP also produces an amino acid change (Lys1069Glu) in the intracellular domain of the protein and segregates independently of rs403578195. These results confirm for the first time the role of the <i>LEPR</i> gene in sheep reproductive seasonality.https://www.mdpi.com/2076-2615/10/12/2448leptin receptorreproductive seasonalityRasa AragonesaSNPhaplotype |
| spellingShingle | Kenza Lakhssassi Malena Serrano Belén Lahoz María Pilar Sarto Laura Pilar Iguácel José Folch José Luis Alabart Jorge Hugo Calvo The <i>LEPR</i> Gene Is Associated with Reproductive Seasonality Traits in Rasa Aragonesa Sheep Animals leptin receptor reproductive seasonality Rasa Aragonesa SNP haplotype |
| title | The <i>LEPR</i> Gene Is Associated with Reproductive Seasonality Traits in Rasa Aragonesa Sheep |
| title_full | The <i>LEPR</i> Gene Is Associated with Reproductive Seasonality Traits in Rasa Aragonesa Sheep |
| title_fullStr | The <i>LEPR</i> Gene Is Associated with Reproductive Seasonality Traits in Rasa Aragonesa Sheep |
| title_full_unstemmed | The <i>LEPR</i> Gene Is Associated with Reproductive Seasonality Traits in Rasa Aragonesa Sheep |
| title_short | The <i>LEPR</i> Gene Is Associated with Reproductive Seasonality Traits in Rasa Aragonesa Sheep |
| title_sort | i lepr i gene is associated with reproductive seasonality traits in rasa aragonesa sheep |
| topic | leptin receptor reproductive seasonality Rasa Aragonesa SNP haplotype |
| url | https://www.mdpi.com/2076-2615/10/12/2448 |
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